Molecular Cloning and Expression in Saccharomyces cerevisiae of Laccase Gene from the Ascomycete Melanocarpus albomyces

Applied and Environmental Microbiology

Volumn 70, Issue 1, 2004, Pages 137-144

  Kiiskinen, Laura Leena ^a   Saloheimo, Markku ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CLONING; DNA; GENES; PROTEINS;

GENE ENCODING;

MICROBIOLOGY;

ALPHA FACTOR GENE; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; ASCOMYCETES; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA DETERMINATION; DNA MODIFICATION; FUNGAL GENE; GENE; GENE CONSTRUCT; GENE EXPRESSION; GENE ISOLATION; INTRON; LAC1 GENE; LACCASE GENE; MELANOCARPUS ALBOMYCES; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN PROCESSING; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; STOP CODON; THERMOPHILIC BACTERIUM;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, FUNGAL; LACCASE; MOLECULAR SEQUENCE DATA; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; SORDARIALES;

ANIMALIA; ASCOMYCOTA; BACTERIA (MICROORGANISMS); FUNGI; MELANOCARPUS; MELANOCARPUS ALBOMYCES; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0346500440     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.1.137-144.2004     Document Type: Article

References (49)

1. Aramayo, R., and W. E. Timberlake. 1990. Sequence and molecular structure of the Aspergillus nidulans yA (laccase I) gene. Nucleic Acids Res. 18:3415.
2. Aramayo, R., and W. E. Timberlake. 1993. The Aspergillus nidulans yA gene is regulated by abaA. EMBO J. 12:2039-2048.
3. Bar Nun, N., A. Tal Lev, E. Harel, and A. M. Mayer. 1988. Repression of laccase formation in Botrytis cinerea and its possible relation to phytopatho-genicity. Phytochemistry 27:2505-2509.
4. Berka, R. M., S. H. Brown, F. Xu, P. Schneider, K. M. Oxenboll, and D. A. Aaslyng. November 1999. Purified Myceliophthora laccases and nucleic acids encoding same. U. S. patent 5,981,243.
5. Berka, R. M., P. Schneider, E. J. Golightly, S. H. Brown, M. Madden, K. M. Brown, T. Halkier, K. Mondorf, and F. Xu. 1997. Characterization of the gene encoding an extracellular laccase of Myceliophtora thermophila and analysis of the recombinant enzyme expressed in Aspergillus oryzae. Appl. Environ. Microbiol. 63:3151-3157.
6. Bourbonnais, R. E., and M. G. Paice. 1990. Oxidation of non-phenolic substrates - an expanded role for laccase in lignin biodegradation. FEBS Lett. 267:99-102.
7. Brown, M. A., Z. Zhao, and A. G. Mauk. 2002. Expression and characterization of a recombinant multi-copper oxidase: laccase IV from Trametes versicolor. Inorg. Chim. Acta 331:232-238.
8. Bulter, T., M. Alcalde, V. Sieber, P. Meinhold, C. Schlachtbauer, and F. H. Arnold. 2003. Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution. Appl. Environ. Microbiol. 69:987-995.
9. Cantone, F. A., and R. C. Staples. 1993. A laccase cDNA from Botrytis cinerea. Phytopathology 83:1383.
10. Cassland, P., and L. J. Jönsson. 1999. Characterization of a gene encoding Trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature. Appl. Microbiol. Biotechnol. 52:393-400.
11. Choi, G. H., T. G. Larson, and D. L. Nuss. 1992. Molecular analysis of the laccase gene from the chestnut blight fungus and selective suppression of its expression in an isogenic hypovirulent strain. Mol. Plant-Microbe Interact. 5:119-128.
12. Claus, H. 2003. Laccases and their occurrence in prokaryotes. Arch. Microbiol. 179:145-150.
13. Eggert, C., P. R. LaFayette, U. Temp, K.-E. L. Eriksson, and J. F. D. Dean. 1998. Molecular analysis of a laccase gene from the white rot fungus Pycnoporus cinnabarinus. Appl. Environ. Microbiol. 64:1766-1772.
14. Fernández-Larrea, J., and U. Stahl. 1996. Isolation and characterization of a laccase gene from Podospora anserina. Mol. Gen. Genet. 252:539-551.
15. 2+-dependent serine protease. Proc. Natl. Acad. Sci. 86:1434-1438.
16. Gelo-Pujic, M., H.-H. Kim, N. G. Butlin, and G. T. R. Palmore. 1999. Electrochemical studies of a truncated laccase produced in Pichia pastoris. Appl. Environ. Microbiol. 65:5515-5521.
17. Germann, U. A., G. Müller, P. E. Hunziker, and K. Lerch. 1988. Characterization of two allelic forms of Neurospora crassa laccase. Amino- and carboxyl-terminal processing of a precursor. J. Biol. Chem. 263:885-896.
18. Gianfreda, L., F. Xu, and J.-M. Bollag. 1999. Laccases: a useful group of oxidoreductive enzymes. Bioremed. J. 3:1-25.
19. Giardina, P., R. Cannio, L. Martitrani, L. Marzullo, G. Palmieri, and G. Sannia. 1995. Cloning and sequencing of a laccase gene from the lignin-degrading basidiomycete Pleurotus ostreatus. Appl. Environ. Microbiol. 61:2408-2413.
20. Gietz, D., A. St. Jean, R. A. Woods, and R. H. Schiestl. 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 20:1425.
21. Hakulinen, N., L.-L. Kiiskinen, K. Kruus, M. Saloheimo, A. Paananen, A. Koivula, and J. Rouvinen. 2002. Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site. Nat. Struct. Biol. 9:601-605.
22. Hatamoto, O., H. Sekine, E. Nakano, and K. Abe. 1999. Cloning and expression of a cDNA encoding the laccase from Schizophyllum commune. Biosci. Biotechnol. Biochem. 63:58-64.
23. Hsieh, H. P., and N. A. Da Silva. 1998. Partial-pKD1 plasmids provide enhanced structural stability for heterologous protein production in Kluyveromyces lactis. Appl. Microbiol. Biotechnol. 49:411-416.
24. Jönsson, L. J., M. Saloheimo, and M. Penttilä. 1997. Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris. Curr. Genet. 32:425-430.
25. Jönsson, L., K. Sjöström, I. Häggström, and P. O. Nyman. 1995. Characterization of a laccase gene from the white-rot fungus Trametes versicolor and structural features of basidiomycete laccases. Biochim. Biophys. Acta 1251:210-215.
26. Kiiskinen, L.-L., L. Viikari, and K. Kruus. 2002. Purification and characterisation of a novel laccase from the ascomycete Melanocarpus albomyces. Appl. Microbiol. Biotechnol. 59:198-204.
27. Kojima, Y., Y. Kita, and Y. Tsukuda. March 1990. DNA for expression and secretion. European patent EP0388166.
28. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
29. Larsson, S., P. Cassland, and L. J. Jönsson. 2001. Development of a Saccharomyces cerevisiae strain with enhanced resistance to phenolic fermentation inhibitors in lignocellulose hydrolysates by heterologous expression of laccase. Appl. Environ. Microbiol. 67:1163-1170.
30. Litvintseva, A. P., and J. M. Henson. 2002. Cloning, characterization, and transcription of three laccase genes from Gaeumannomyces graminis var. tritici, the take-all fungus. Appl. Environ. Microbiol. 68:1305-131.
31. Martins, L. O., C. M. Soares, M. M. Pereira, M. Teixeira, T. Costa, G. H. Jones, and A. O. Henriques. 2002. Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat. J. Biol. Chem. 277:18849-18859.
32. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10:1-6.
33. Niku-Paavola, M.-L., E. Karhunen, P. Salola, and V. Raunio. 1988. Ligninolytic enzymes of the white-rot fungus Phlebia radiata. Biochem. J. 254:877-884.
34. Otterbein, L., E. Record, S. Longhi, M. Asther, and S. Moukha. 2000. Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus 1-937 and expression in Pichia pastoris. Eur. J. Biochem. 267:1619-1625.
35. Raeder, U. A., and P. Broda. 1985. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1:17-20.
36. Record, E., P. J. Punt, M. Chamkha, M. Labat, C. A. M. J. J. van Den Hondel, and M. Asther. 2002. Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme. Eur. J. Biochem. 269:602-609.
37. Saloheimo, A., B. Henrissat, A.-M. Hoffrén, O. Teleman, and M. Penttilä. 1994. A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast. Mol. Microbiol. 13:219-228.
38. Saloheimo, M., and M.-L. Niku-Paavola. 1991. Heterologous production of a ligninolytic enzyme: expression of the Phlebia radiata laccase gene in Trichoderma reesei. Bio/Technology 9:987-990.
39. Saloheimo, M., M.-L. Niku-Paavola, and J. K. Knowles. 1991. Isolation and structural analysis of the laccase gene from the lignin-degrading fungus Phlebia radiata. J. Gen. Microbiol. 37:1537-1544.
40. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
41. Sherman, F. 1991. Getting started with yeast. Methods Enzymol. 194:3-21.
42. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
43. Thurston, C. 1994. The structure and function of fungal laccases. Microbiology 140:19-26.
44. Tsuji, G., J. Fujikawa, H. Ishisa, O. Horino, and Y. Kubo. 2001. Laccase gene LAC1 of Colletotrichum lagenarium is not essential for melanin biosynthesis and pathogenicity. J. Gen. Plant Pathol. 67:182-190.
45. Weydemann, U., P. Keup, M. Piontek, A. W. M. Strasser, J. Schweden, G. Gellissen, and Z. A. Janowicz. 1995. High-level secretion of hirudin by Hansenula polymorpha-authentic processing of three different preprohirudins. Appl. Microbiol. Biotechnol. 44:377-385.
46. Williamson, P. R. 1994. Biochemical and molecular characterization of the diphenol oxidase of Cryptococcus neofonnans: identification as a laccase. J. Bacteriol. 176:656-664.
47. Xu, F. 1999. Recent progress in laccase study: properties, enzymology, production, and applications, p. 1545-1554. In M. C. Flickinger and S. W. Drew (ed.), The encyclopedia of bioprocessing technology: fermentation, biocatalysis and bioseparation. John Wiley & Sons, New York, N.Y.
48. Yaver, D. S., M. J. Overjero, F. Xu, B. A. Nelson, K. M. Brown, T. Halkier, S. Bernauer, S. H. Brown, and S. Kauppinen. 1999. Molecular characterization of laccase genes from the basidiomycete Coprinus cinercus and heterologous expression of the laccase Lccl. Appl. Environ. Microbiol. 65:4943-4948.
49. Yaver, D. S., F. Xu, E. J. Golightly, K. M. Brown, S. H. Brown, M. W. Rey, P. Schneider, T. Halkier, K. Mondorf, and H. Dalboge. 1996. Purification, characterization, molecular cloning, and expression of two laccase genes from the white rot basidiomycete Trametes villosa. Appl. Environ. Microbiol. 62:834-841.