Electrochemical studies of a truncated laccase produced in Pichia pastoris

Applied and Environmental Microbiology

Volumn 65, Issue 12, 1999, Pages 5515-5521

  Gelo Pujic, Mirjana ^a   Kim, Hyug Han ^a   Butlin, Nathan G ^a   Palmore, G Tayhas R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; COPPER; CYSTEINE; HYDROGEN; LACCASE; OXIDOREDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CYCLIC POTENTIOMETRY; ELECTROCHEMISTRY; ELECTRODE; ELECTRON TRANSPORT; NONHUMAN; OXIDATION REDUCTION REACTION; PICHIA PASTORIS;

EID: 0032731965     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.12.5515-5521.1999     Document Type: Article

References (54)

1. Allen, P. M., H. A. O. Hill, and N. J. Walton. 1984. Surface modifiers for the promotion of direct electrochemistry of cytochrome c. J. Electroanal. Chem. 178:69.
2. Andreásson, L.-E., R. Branden, and B. Reinhammar. 1976. Kinetic studies of Rhus vernicifera laccase. Evidence for multi-electron transfer and an oxygen intermediate in the reoxidation reaction. Biochim. Biophys. Acta 438:370-379.
3. Andreásson, L.-E., and B. Reinhammar. 1976. Kinetic studies of Rhus vernicifera laccase: role of the metal centers in electron transfer. Biochim. Biophys. Acta 445:579-597.
4. Andreásson, L.-E., and B. Reinhammar. 1979. The mechanism of electron transfer in laccase-catalyzed reactions. Biochim. Biophys. Acta 568:145-156.
5. Bard, A. J., and L. R. Faulkner. 1980. Electrochemical methods. John Wiley & Sons, Inc., New York, N.Y.
6. Bar-Nun, S., Y. Shneyour, and J. S. Beckmann. 1983. G-418, an elongation inhibitor of 80S ribosomes. Biochim. Biophys. Acta 741:123-127.
7. Bergens, S. H., C. B. Gorman, G. T. R. Palmore, and G. M. Witesides. 1994. A redox fuel cell that operates with methane as fuel at 120°C. Science 265:1418-1420.
8. Bourbonnais, R., M. G. Paice, I. D. Reid, P. Lanthier, and M. Yaguchi. 1995. Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate) in kraft lignin depolymerization. Appl. Environ. Microbiol. 61:1876-1880.
9. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
10. Brett, C. M. A., and A. M. O. Brett. 1993. Electrochemistry principles, methods, and applications. Oxford Science Publications, New York, N.Y.
11. Cass, A. E. G., G. Davis, G. D. Francis, H. A. O. Hill, W. J. Aston, I. J. Higgins, V. Plotkin, L. D. L. Scott, and A. P. F. Turner. 1984. Ferrocene-mediated enzyme electrode for amperometric determination of glucose. Anal. Chem. 56:667-671.
12. Childs, R. E., and W. G. Bardsley. 1975. The steady-state kinetics of peroxidase with 2,2′-azino-di-(3-ethylbenzthiazoline-6-sulphonic acid) as chromogen. Biochem. J. 145:93-103.
13. Clare, J., C. Scorer, R. Buckholz, and M. Romanos. 1998. Expression of EGF and HIV envelope glycoprotein, p. 222. In D. R. Higgins and J. M. Cregg (ed.), Pichia protocols. Humana Press, Totowa, N.J.
14. Cullen, D. 1997. Recent advances on the molecular genetics of ligninolytic fungi. J. Biotechnol. 53:273-289.
15. Degani, Y., and A. Heller. 1987. Direct electrical communication between chemically modified enzymes and metal electrodes. 1. Electron transfer from glucose oxidase to metal electrodes via electron relays, bound covalently to the enzyme. J. Phys. Chem. 91:1285-1289.
16. Degani, Y., and A. Heller. 1988. Direct electrochemical communication between chemically modified enzymes and metal electrodes. 2. Methods for bonding electron-transfer relays to glucose oxidase and D-amino acid oxidase. J. Am. Chem. Soc. 110:2615-2620.
17. Ducros, V., A. M. Brzozowski, K. S. Wilson, S. H. Brown, P. Østergaard, P. Schneider, D. S. Yaver, A. H. Pedersen, and G. J. Davies. 1998. Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 Å resolution. Nat. Struct. Biol. 5:310-316.
18. Givaudan, A., A. Effosse, D. Faure, P. Potier, M.-L. Bouillant, and R. Bally. 1993. Polyphenol oxidase in azospirillum-lipoferum isolated from rice rhizosphere - evidence for laccase activity in non-motile strains of Azospirillum-Lipoferum. FEMS Microbiol. Lett. 108:205-210.
19. Guckert, J. A., M. D. Lowery, and E. I. Solomon. 1995. Electronic structure of the reduced blue copper active site - contributions to the reduction potentials and geometry. J. Am. Chem. Soc. 117:2814-2844.
20. Hill, H. A. O. 1996. The development of bioelectrochemistry. Coord. Chem. Rev. 151:115-123.
21. Hyung, K. H., K. Y. Jun, H.-G. Hong, Y. S. Kim, and W. Shin. 1997. Immobilization of laccase onto the gold electrode using β-mercaptopropionate. Bull. Korean Chem. Soc. 18:564-566.
22. Invitrogen. 1998. Multi-copy Pichia expression kit manual, version C. Invitrogen Corporation, Carlsbad, Calif.
23. Jönsson, L., K. Sjostrom, I. Haggstrom, and P. O. Nyman. 1995. Characterization of a laccase gene from the white-rot fungus Trametes versicolor and structural features of basidiomycete laccases. Biochem. Biophys. Acta 1251: 210-215.
24. Jönsson, L. J., M. Saloheimo, and M. Penttilä. 1997. Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris. Curr. Genet. 32:425-430.
25. Kojima, Y., Y. Tsukuda, Y. Kawai, A. Tsukamoto, J. Sugiura, M. Sakaino, and Y. Kita. 1990. Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus. J. Biol. Chem. 265:15224-15230.
26. Küng, M., B. Stadelmann, J. Brodbeck, and P. Bütikofer. 1997. Addition of G418 and other aminoglycoside antibiotics to mammalian cells results in the release of GPI-anchorcd proteins. FEBS Lett. 409:333-338.
27. Kuznertsov, A. M., V. A. Bogdanovskaya, M. R. Tarasevich, and E. F. Gavrilova. 1987. The mechanism of cathode reduction of oxygen in a carbon carrier-laccase system. FEBS Lett. 215:219-222.
28. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
29. Lee, C.-W., H. B. Gray, F. C. Anson, and B. G. Malmström. 1984. Catalysis of the reduction of dioxygen at graphite electrodes coated with fungal laccase A. J. Electroanal. Chem. 172:289-300.
30. Messerschmidt, A., R. Ladenstein, R. Huber, M. Bolognesi, L. Avigliano, R. Petruzzelli, A. Rossi, and A. Finazzi-Agro. 1992. Refined crystal structure of ascorbate oxidase at 1.9 Å resolution. J. Mol. Biol. 224:179-205.
31. Mikuni, J., and N. Morohoshi. 1977. Cloning and sequencing of a second laccase gene from the white-rot fungus Coriolus versicolor. FEMS Microbiol. Lett. 155:79-84.
32. Mosbach, R. 1963. Purification and some properties of laccase from Polyporus versicolor. Biochim. Biophys. Acta 73:204-212.
33. Nicholson, R. S. 1965. Theory and application of cyclic voltammetry for measurement of electrode-reaction kinetics. Anal. Chem. 37:1351-1355.
34. Nicholson, R. S., and I. Shain. 1964. Theory of stationary electrode polarography: single sain and cyclic methods applied to reversible, irreversible, and kinetics systems. Anal. Chem. 36:706-722.
35. Ong, E., B. Pollock, and M. Smith. 1996. GenBank accession no. U44430.
36. Ong, E., W. B. R. Pollock, and M. Smith. 1997. Cloning and sequence analysis of two laccase complementary DNAs from the ligninolytic basidiomycete Trametes versicolor. Gene 196:113-119.
37. Palmore, G. T. R., H. Bertschy, S. H. Bergens, and G. M. Whitesides. 1998. A methanol/dioxygen biofuel cell that uses NAD'-dependent dehydrogenases as catalysts: application of an electro-enzymatic method to regenerate nicotinamide adenine dinucleotide at low overpotentials. J. Electroanal. Chem. 443:155-161.
38. Palmore, G. T. R., and H.-H. Kim. 1999. Electro-enzymatic reduction of dioxygen to water in the cathode compartment of a biofuel cell. J. Electroanal. Chem. 464:110-117.
39. Palmore, G. T. R., and G. M. Whitesides. 1994. Microhial and enzymatic biofuel cells, p. 271-290. In M. E. Himmel, J. O. Baker, and R. P. Overend (ed.), Enzymatic conversion of biomass for fuels production. ACS Symposium Series. American Chemical Society, Washington, D.C.
40. Reinhammar, B. 1981. An EPR signal from the half-reduced type 3 copper pair in Rhus vernicifera laccase. J. Inorg. Biochem. 15:27-39.
41. Reinhammar, B. R. M. 1972. Oxidation-reduction potentials of the electron acceptors in laccase and stellacyanin. Biochim. Biophys. Acta 275:245-259.
42. Rescigno, A., E. Sanjust, L. Montanari, F. Sollai, G. Soddu, A. C. Rinaldi, S. Oliva, and A. Rinaldi. 1997. Detection of laccase, peroxidase and polyphenol oxidase on a single polyacrylamide gel electrophoresis. Anal. Lett. 30:2211-2220.
43. Solomon, E. I., T. E. Machonkin, and U. M. Sundaram. 1997. Spectroscopy of multi-copper oxidases, p. 103-128. In A. Messerschmidt (ed.), Multi-copper oxidases. World Scientific, River Edge, N.J.
44. Taniguchi, I., M. Iseki, H. Yamaguchi, and K. Yasykouchi. 1984. Surface enhance Raman scattering study of horse heart cytochrome c at a silver electrode in the presence of bis(4-pyridyl)disulfide and purine. J. Electroanal. Chem. 175:341.
45. Tarasevich, M. R., A. I. Yaropolov, V. A. Bogdanovskaya, and S. D. Varfolomeev. 1979. Electrocatalysis of a cathodic reduction by laccase. J. Electroanal. Chem. 104:393-403.
46. Thuesen, M. H., O. Farver, B. Reinhammar, and J. Ulstrup. 1998. Cyclic voltammetry and electrocatalysis of the blue copper oxidase polyporus versicolor laccase. Acta Chem. Scand. 52:555-562.
47. Xu, F., R. M. Berka, J. A. Wahleithner, B. A. Nelson, J. R. Shuster, S. H. Brown, A. E. Palmer, and E. I. Solomon. 1998. Site-directed mutations in fungal laccase: effect on redox potential, activity and pH profile. Biochem. J. 334:63-70.
48. Xu, F., A. E. Palmer, D. S. Yaver, R. M. Berka, G. A. Gambetta, S. H. Brown, and E. I. Solomon. 1999. Targeted mutation in a Trametes villosa laccase. J. Biol. Chem. 274:12372-12375.
49. Yaropolov, A. I., A. N. Kharybin, J. Emnéus, G. Marko-Varga, and L. Gorton. 1996. Electrochemical properties of some copper-containing oxidases. Bioelectrochem. Bioenerg. 40:49-57.
50. Yaver, D. S., and E. J. Golightly. 1996. Cloning and characterization of three laccase genes from the white-rot basidiomycete Trametes villosa: genomic organization of the laccase gene family. Gene 181:95-102.
51. Yaver, D. S., F. Xu, H. Dalboge, P. Schneider, and D. A. Aaslyng. 4 January 1996. U.S. Patent WO 96/00290.
52. Yaver, D. S., F. Xu, E. J. Golightly, K. M. Brown, S. H. Brown, M. W. Rey, P. Schneider, T. Halkier, K. Mondorf, and H. Dalboge. 1996. Purification, characterization, molecular cloning, and expression of two laccase genes from the white rot basidiomycete Trametes villosa. Appl. Environ. Microbiol. 62:834-841.
53. Yoneyama, H., and Y. Kajiya. 1993. Achievement of direct electron transfer between glucose oxidase and an electrode by adsorption of hydroquinone-sulfate on the enzyme. Sens. Actuators B 13:65-67.
54. Youn, H.-D., K.-J. Kim, J.-S. Maeng, Y.-H. Han, I.-B. Jeong. G. Jeong, S.-O. Kang, and Y. C. Hah. 1995. Single electron transfer by an extracellular laccase from the white-rot fungus Pleurotus ostreatus. Microbiology 141:393-398.