Molecular cloning of the cDNA encoding laccase from Trametes versicolor and heterologous expression in Pichia methanolica

Applied Microbiology and Biotechnology

Volumn 69, Issue 2, 2005, Pages 178-183

  Guo, Mei ^a   Lu, Fuping ^b   Pu, Jun ^b   Bai, Dongqing ^a   Du, Lianxiang ^b  

^a TIANJIN AGRICULTURAL UNIVERSITY   (China)

^b TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CLONING; DNA; FUNGI; MOLECULAR BIOLOGY; PROTEINS;

ISOENZYME; LACCASE; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

ENZYMES;

AMINO ACID; COMPLEMENTARY DNA; COPPER; ISOENZYME; LACCASE; MATING HORMONE ALPHA FACTOR; RECOMBINANT PROTEIN; RNA; SIGNAL PEPTIDE;

GENE EXPRESSION;

ARTICLE; CONTROLLED STUDY; DNA ISOLATION; ENZYME ACTIVITY; EXTRACELLULAR MATRIX; GENE VECTOR; GENETIC CODE; HETEROLOGOUS EXPRESSION; IMMUNODETECTION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PICHIA; PICHIA METHANOLICA; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE; SPECIES CULTIVATION; TEMPERATURE; TRAMETES VERSICOLOR; WESTERN BLOTTING;

BLOTTING, WESTERN; CLONING, MOLECULAR; COPPER; DNA, COMPLEMENTARY; DNA, FUNGAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; LACCASE; PICHIA; POLYPORALES; TEMPERATURE;

FUNGI; PICHIA; PICHIA METHANOLICA; SACCHAROMYCES CEREVISIAE; TRAMETES VERSICOLOR;

EID: 29144530586     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-1985-3     Document Type: Article

References (19)

1. Archibald FS (1992) Lignin perixidase activity is not important in biological bleaching and delignification of unbleached kraft pulp by Trametes versicolor. Appl Environ Microbiol 58:3101-3109
2. Cassland P, Mnsson LJ (1999) Characterization of a gene encoding Trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature. Appl Microbiol Biotechnol 52:393-400
3. Choi J, Kim EE (2002) Expression of the active human and duck hepatitis B virus polymerases in heterologous system of Pichia methanolica. Antivir Res 55:279-290
4. Durrens P (1981) The phenoloxidases of the ascomycete Podospora anserina: the three forms of the major laccase activity. Arch Microbiol 130:121-124
5. Fahraeus G, Reinhammar B (1967) Large scale production and purification of laccase from the fungus Polyporus versicolor and some properties of laccase. Acta Chem Scand 21:2367-2378
6. Gianfreda L, Xu F, Bollag JM (1999) Laccase: a useful group of oxidoreductive enzymes. Biorem J 3:1-25
7. Hatakka A (1994) Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation. FEMS Microbiol Rev 13:125-135
8. Jönsson L, Saloheimo M, Penttila M (1997) Laccase from the white-rot fungus Trametes versicolor. cDNA cloning of lcc1 and expression in Pichia pastoris. Curr Genet 32:425-430
9. Kjeldsen T, Pettersson AF, Hach M (1999) Secretory expression and characterization of insulin in Pichia pastoris. Biotechnol Appl Biochem 29:79-86
10. Kojima Y, Tsukuda Y, Kawai Y, Tsukamoto A, Sugiura J, Sakaino M, Kita Y (1990) Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus. J Biol Chem 265:15224-15230
11. Liu W, Chao Y, Liu S, Bao H, Qian S (2003) Molecular cloning and characterization of a laccase gene from the basidiomycete Fome lignosus and expression in Pichia pastoris. Appl Microbiol Biotechnol 63:174-181
12. Messerschmidt A (1993) Blue copper oxidases. Adv Inorg Chem 40:121-185
13. O'Callaghan J, O'Brien MM, Dobson ADW (2002) Optimisation of the expression of Trametes versicolor laccase gene in Pichia pastoris. J Ind Microbiol Biotechnol 29:55-59
14. Paice MG, Reid ID, Bourbinnais R, Archibald FS, Jurasek L (1993) Manganese peroxidase, produced by Trametes versicolor during pulp bleaching, demethylates and delignifies kraft pulp. Appl Environ Microbiol 59:260-265
15. Roy-Arcand L, Archibald FS (1991) Direct dechlorination of chlorophenolic compounds by laccases from Trametes (Coriolus) versicolor. Enzyme Microb Technol 13:194-203
16. Schein CH, Noteborn MHM (1988) Formation of soluble recombinant proteins in Escherichia coli is favored by lower growth temperature. Biotechnology 6:291-294
17. Thurston CF (1994) The structure and function of fungal laccase. Microbiologist 140:19-26
18. Wood DA (1998) Production, purification and properties of extracellular laccase of Agaricus bisporus. J Gen Microbiol 333:2527-2534
19. Yaver DS, Xu F, Golightly EJ, Brown SH, Rey MW, Schneider P, Halkier T, Mondorf K, Dallboge H (1996) Purification, characterization, molecular cloning and expression of two laccases from the white rot basidiomycete Trametes villosa. Appl Environ Microbiol 62:834-841