Genetic analysis of G protein-coupled receptor expression in Escherichia Coli: Inhibitory role of DnaJ on the membrane integration of the human central cannabinoid receptor

Biotechnology and Bioengineering

Volumn 102, Issue 2, 2009, Pages 357-367

  Skretas, Georgios ^a,b   Georgiou, George ^a,b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Dnaj; Escherichia coli; Fluorescence activated cell sorting; G protein coupled receptor; Genetic engineering; Membrane protein

Indexed keywords

BACTERIAL PRODUCTIONS; CANNABINOID RECEPTORS; CELL TOXICITIES; DNAJ; E. COLI; FLUORESCENCE-ACTIVATED CELL SORTING; G PROTEIN-COUPLED RECEPTOR; GENETIC ANALYSIS; GREEN FLUORESCENT PROTEINS; INCLUSION BODIES; INTEGRAL MEMBRANE PROTEINS; MEMBRANE INSERTIONS; MEMBRANE PROTEIN; OVER EXPRESSIONS; WILD TYPES;

CELL MEMBRANES; ESCHERICHIA COLI; FLUORESCENCE; GENETIC ENGINEERING; MEMBRANES; PROTEINS;

PROTEIN FOLDING;

CANNABINOID RECEPTOR; PROTEIN DNAJ; CANNABINOID 1 RECEPTOR; DNAJ PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; G PROTEIN COUPLED RECEPTOR; GREEN FLUORESCENT PROTEIN; HEAT SHOCK PROTEIN 40; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; BACTERIAL MEMBRANE; BACTERIUM ISOLATION; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTING; GENE INSERTION; GENETIC ANALYSIS; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN SYNTHESIS; TRANSPOSON; BIOSYNTHESIS; CELL MEMBRANE; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; HUMAN; METABOLISM; NUCLEOTIDE SEQUENCE; PHYSIOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

CELL MEMBRANE; DNA MUTATIONAL ANALYSIS; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENGINEERING; GREEN FLUORESCENT PROTEINS; HSP40 HEAT-SHOCK PROTEINS; HUMANS; MUTAGENESIS, INSERTIONAL; PROTEIN BIOSYNTHESIS; RECEPTOR, CANNABINOID, CB1; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS;

EID: 58249084813     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22097     Document Type: Article

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