Combined effects of the signal sequence and the major chaperone proteins on the export of human cytokines in Escherichia coli

Applied and Environmental Microbiology

Volumn 62, Issue 1, 1996, Pages 55-60

  Bergès, Hélène ^a   Joseph Liauzuk, Evelyne ^b   Fayet, Olivier ^a  

^a CNRS   (France)

^b SANOFI   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONE; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; HYBRID PROTEIN; INTERLEUKIN 13; SIGNAL PEPTIDE;

ARTICLE; CELL GROWTH; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; IMMUNOBLOTTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; RNA TRANSLATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030023780     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.1.55-60.1996     Document Type: Article

References (29)

1. Altman, E., C. A. Kumamoto, and S. D. Emr. 1991. Heat-shock proteins can substitute for SecB function during protein export in E. coli. EMBO J. 10:239-245.
2. Aral, K., F. Lee, A. Miyajima, S. Mistake, N. Aral, and T. Yokota. 1990. Cytokines, coordinators of immune and inflammatory responses. Annu. Rev. Bioehem. 59:783-836.
3. Bardwell, J. C. A. 1994. Building bridges: disulphide bond formation in the cell. Mill. Microbiol. 14:199-205.
4. Blum, P., M. Velligan, N. Lin, and A. Matin. 1992. DnaK-mediated alterations in human growth hormone protein inclusion bodies. Bio/Technology 10:301-304.
5. Coffey, R. G. 1992. Granulocyte responses to cytokines: basic and clinical research. Marcel Dekker. Inc., New York.
6. Duenas M., J. Vasquez, M. Ayala, E. Soderlind, M. Ohlin, L. Pérez, C. A. K. Borrebaeck, and J. V. Gavilondo. 1994. Intra- and extracellular expression of an scFv antibody fragment in E. coli: effect of bacterial strains and pathway engineering using GroES/L chaperonins. BioTechniques 16:476-483.
7. Fayet, O., J. M. Louarn, and C. Georgopoulos. 1986. Suppression of the E. coli dnaA46 mutation by amplification of the groES and groEL genes. Mol. Gen. Genet. 202:435-445.
8. Hardy, S. J. S., and L. L. Randall, 1991. A kinetic partitioning model of selective binding of normative proteins by the bacterial chaperone SecB. Science 251:439-443.
9. Harlow, E., and D. Lane. 1988. Antibodies. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
10. Hartl, F. H., J. Martin, and W. Neupert. 1992. Protein folding in the cell: the role of molecular chaperones Hsp70 and Hsp60. Annu. Rev. Biophys. Biomol. Struct. 21:293-322.
11. Izard, J. W., and D. A. Kendall. 1994. Signal peptides: exquisitely designed transport promoters. Mol. Microbiol. 13:765-773.
12. Joseph-Liauzun, E., P. Legoux, p. Leplatois, G. Loison, and W. Roskam. January 1992. Signal peptide, DNA sequences coding for the latter, expression vectors carrying one of these sequences, gram negative bacteria transformed by these vectors and process for the periplasmic production of a polypeptide. U.S. patent 5,284,768.
13. Joseph-Liauzun, E., P. Leplatois, R. Legoux, V. Guerveno, E. Marchese, and P. Ferrara. 1990. Human recombinant interleukin-1B isolated from Escherichia coli by simple osmotic shock. Gene 86:291-295.
14. Kumamoto, C. A. 1989. Escherichia coli SecB protein associates with exported protein precursors in vivo. Proc. Natl. Acad. Sci. USA 86:53211-5324.
15. Kusukawa, N., T. Yura, C. Ueguchi, Y. Akiyama, and K. lto. 1989. Effects of mutations in heat-shock genes groES and groEL on protein export in E. coli. EMBO J. 8:3517-3521.
16. 15a.Labit-Le Bouteiller, C. Personal communication.
17. Labit-Le Bouteiller, C., R. Astruc, A. Minty, p. Ferrara, and J. Lupker. 1995 Isolation of an IL13 dependent sub-clone of the B9 cell line useful for the estimation of human IL13 bioactivity. J. Immunol. Methods 181:29-36.
18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature (London) 227:680-685.
19. Lee, S. C., and P. O. Olins. 1992. Effect of overproduction of heat-shock chaperones GroESL and DnaK on human procollagenase production in E. coli. J. Biol. Chem. 5:2849-2852.
20. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
21. Minty, A., P. Chalon, X. Dumont, J. C. Guillemot, M. Kaghad, C. Labit, p. Leplatois, p. Liauzun, B. Miloux, C. Minty, p. Casellas, G. Loison, J. Lupker, D. Shire, p. Ferrara, and D. Caput. 1993. lnterleukin-13 is a new human lymphokine regulating inflammatory and immune responses. Nature (London) 362:248-250.
22. Missiakas, D., C. Georgopoulos, and S. Raina, 1993. The Escherichia coli heat shock gene htpY: mutational analysis, cloning, sequencing, and transcriptional regulation. J. Bacteriol. 175:2613-2624.
23. Phillips, G. J., and T. J. Silhavy. 1990. Heal shock proteins DnaK and GroEL facilitate export of LacZ hybrid proteins in E. coli. Nature (London) 344:882-884.
24. Pugsley, A. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
25. Schekman, R. 1994. Translocation gets a push. Cell 78:911-913.
26. Solioz, M., and D. Bienz. 1990. Bacterial genetics by electric shock. Trends Biochem. Sci. 15:175-177.
27. Sprengart, M. L., H. P. Fatscher, and E. Fuchs. 1990. The initiation of translation in E. coli: apparent base-pairing between the 16S-rRNA and downstream sequence of the mRNA. Nucleic Acids Res. 18:1719-1723.
28. Weiss, J. B., P. H. Ray, p. J. Bassford, Jr. 1988. Purified SecB protein of E. coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro. Proc. Natl. Acad. Sci. USA 85:8978-8982.
29. Wild, J., E. Altman, T. Yura, and C. C. Gross. 1992 DnaK and DnaJ heat-shock proteins participate in protein export in E. coli. Genes Dev. 6:1165-1172.