Site-directed mutagenesis combined with oxidative methionine labeling for probing structural transitions of a membrane protein by mass spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 21, Issue 11, 2010, Pages 1947-1956

  Pan, Yan ^a   Brown, Leonid ^b   Konermann, Lars ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

^b UNIVERSITY OF GUELPH   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIORHODOPSIN (BR); HYDROXYL RADICALS; LOOP RESIDUES; MEMBRANE PROTEINS; METHIONINE SULFOXIDE; NATIVE CONFORMATION; NATIVE STATE; OXIDATION BEHAVIORS; OXIDATIVE PATHWAYS; PEPTIDE MAPPING; SITE DIRECTED MUTAGENESIS; SOLVENT ACCESSIBILITY; STRUCTURAL TRANSITIONS;

AMINO ACIDS; BIOLOGICAL MEMBRANES; MASS SPECTROMETRY;

PROTEINS;

BACTERIORHODOPSIN; METHIONINE;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL LABELING; COLLISIONALLY ACTIVATED DISSOCIATION; GENETIC VARIABILITY; HALOBACTERIUM SALINARUM; MASS SPECTROMETRY; MOLECULAR DYNAMICS; NONHUMAN; OXIDATION; PEPTIDE MAPPING; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; BACTERIORHODOPSINS; CRYSTALLOGRAPHY, X-RAY; DEUTERIUM; HALOBACTERIUM SALINARUM; METHIONINE; MOLECULAR SEQUENCE ANNOTATION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; SODIUM DODECYL SULFATE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 77958198646     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2010.08.004     Document Type: Article

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