From structure to mechanism: Electron crystallographic studies of bacteriorhodopsin

Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences

Volumn 360, Issue 1794, 2002, Pages 859-874

  Subramaniam, Sriram ^a   Hirai, Teruhisa ^a   Henderson, Richard ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Conformational change; Electron microscopy; Image analysis; Proton pump; Two dimensional crystal

Indexed keywords

BACTERIORHODOPSIN;

CHEMICAL STRUCTURE; CHEMISTRY; ELECTRON MICROSCOPY; LIGHT; METHODOLOGY; PHYSIOLOGY; PROTEIN CONFORMATION; RADIATION EXPOSURE; REVIEW; STRUCTURE ACTIVITY RELATION; ULTRASTRUCTURE; X RAY CRYSTALLOGRAPHY;

BACTERIORHODOPSINS; CRYSTALLOGRAPHY, X-RAY; LIGHT; MICROSCOPY, ELECTRON; MODELS, MOLECULAR; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0037093403     PISSN: 1364503X     EISSN: None     Source Type: Journal    
DOI: 10.1098/rsta.2001.0971     Document Type: Article

References (43)

1. Trapping and spectroscopic identification of the photointermediates of bacteriorhodopsin at low temperatures
2. Titration of aspartate-85 in bacteriorhodopsin: What it says about chromophore isomerization and proton release
3. Protein, lipid and water organization in bacteriorhodopsin crystals: A molecular view of the purple membrane at 1.9 Angstrom resolution
4. Spectroscopy of the bacteriorhodopsin mutant Tyr-185 → Phe: Asp-96 reprotonates during O formation; Asp-85 and Asp-212 deprotonate during O decay
5. Molecular mechanism of protein-retinal coupling in bacteriorhodopsin
6. Reducing the flexibility of retinal restores a wild-type-like photocycle in baeteriorhodopsin mutants defective in protein-retinal coupling
7. Structural changes in bacteriorhodopsin during proton translocation revealed by neutron diffraction
8. Light energy transduction in bacteriorhodopsin
9. High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle
10. Lipid patches in membrane protein oligomers: Crystal structure of the bacteriorhodopsin-lipid complex
11. Electroncrystallographic refinement of the structure of bacteriorhodopsin
12. Closing in on bacteriorhodopsin: Progress in understanding the molecule
13. Three-dimensional model of purple membrane obtained by electron microscopy
14. Model for the structure of bacteriorhodopsin based on high-resolution electron cryomicroscopy
15. Simple allosteric model for membrane pumps
16. The last phase of the reprotonation switch in bacteriorhodopsin: The transition between the M-type and the N-type protein conformation depends on hydration
17. Bacteriorhodopsin, a membrane protein that uses light to translocate protons
18. Surface of baeteriorhodopsin revealed by high-resolution electron crystallography
19. Time-resolved X-ray diffraction study of structural changes associated with the photocycle of bacteriorhodopsin
20. Proton translocation mechanism and energetics in the light-driven pump bacteriorhodopsin
21. Bacteriorhodopsin
22. Bacteriorhodopsin: A light-driven proton pump in Halobacterium halobium
23. Proton transfer pathways in bacteriorhodopsin at 2.3 Å resolution
24. Structural changes in bacteriorhodopsin during ion transport at 2 Å resolution
25. Structure of bacteriorhodopsin at 1.55 Angstrom resolution
26. Coupling photoisomerization of retinal to directional transport in bacteriorhodopsin
27. Structures of aspartic acid-96 in the L-intermediate and N-intermediate of bacteriorhodopsin - Analysis by Fourier-transform infrared-spectroscopy
28. The structure of bacteriorhodopsin at 3.0 Å resolution based on electron crystallography: Implication of the charge distribution
29. Crystallographic characterization by X-ray diffraction of the M intermediate from the photocycle of bacteriorhodopsin at room temperature
30. Microbial genome analyses: Global comparisons of transport capabilities based on phylogenies, bioenergetics and substrate specificities
31. X-ray structure of bacteriorhodopsin at 2.5 Angstroms from microcrystals grown in lipidic cubic phases
32. pK of the protonated Sehiff-base and aspartic-85 in the bacteriorhodopsin binding-site is controlled by a specific geometry between the two residues
33. Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsin
34. Environmental effects on the protonation states of active site residues in bacteriorhodopsin
35. Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin
36. Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin
37. Bacteriorhodopsin and related pigments of halobacteria
38. The structure of bacteriorhodopsin: An emerging consensus
39. Molecular mechanism of vectorial proton translocation by bacteriorhodopsin
40. Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin
41. Electron diffraction studies of light-induced conformational changes in the Leu-93 → Ala bacteriorhodopsin mutant
42. Protein conformational changes in the bacteriorhodopsin photocycle
43. Structure of the bacteriorhodopsin mutant F219L N intermediate revealed by electron crystallography