Physico-chemical determinants of soluble intrabody expression in mammalian cell cytoplasm

Protein Engineering, Design and Selection

Volumn 23, Issue 6, 2010, Pages 489-498

  Kvam, Erik ^a,b   Sierks, Michael R ^c   Shoemaker, Charles B ^d   Messer, Anne ^a,b  

^a WADSWORTH CENTER   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

^c Arizona State University   (United States)

^d TUFTS UNIVERSITY   (United States)

Author keywords

intrabody; intracellular antibody; protein aggregation; scFv; VHH

Indexed keywords

ANTIBODY FRAGMENT; ANTIBODY LIBRARY; DIAGNOSTIC AGENTS; DISULFIDE BOND FORMATION; EXTRACELLULAR; FUNCTIONAL GENOMICS; HYDROPHOBIC INTERACTIONS; IN-CELL; INTRACELLULAR ANTIBODY; IONIC REPULSION; MACROMOLECULAR CROWDING; MAMMALIAN CELLS; NEGATIVE CHARGE; PEPTIDE TAGS; PHYSICO-CHEMICALS; POSITIVELY CHARGED; PROOF OF PRINCIPLES; PROTEIN AGGREGATION; PROTEOMICS; REDOX POTENTIALS; SEQUENCE ANALYSIS; SEQUENCE DETERMINANTS; SOLUBLE EXPRESSION; VARIABLE FRAGMENT;

CELLS; CHEMICAL BONDS; CYTOLOGY; ENZYME ACTIVITY; GENE THERAPY; GENETIC ENGINEERING; HYDROPHOBICITY; MAMMALS; MOLECULAR BIOLOGY; REDOX REACTIONS; SOLUBILITY;

ANTIBODIES;

SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ACIDIFICATION; ANIMAL CELL; ANTIBODY LIBRARY; ARTICLE; ARTIODACTYLA; BACTERIAL GENE; CELL INTERACTION; CELL PH; CONTROLLED STUDY; CROWDING; CYTOPLASM; DISULFIDE BOND; ELECTRICITY; FUNCTIONAL GENOMICS; GENE THERAPY; HYDROPHILICITY; MACROMOLECULE; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION POTENTIAL; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SIMIAN VIRUS 40; SOLUBILITY;

AMINO ACID SEQUENCE; ANALYSIS OF VARIANCE; ANIMALS; CAMELIDS, NEW WORLD; CELL LINE; CYTOPLASM; DATABASES, PROTEIN; GREEN FLUORESCENT PROTEINS; HUMANS; HYDROGEN-ION CONCENTRATION; HYDROPHOBICITY; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN MULTIMERIZATION; RATS; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; SINGLE-CHAIN ANTIBODIES; SOLUBILITY;

CAMELIDAE; MAMMALIA;

EID: 77955190783     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq022     Document Type: Article

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