Crystal structure of a complex between the actinomadura R39 dd -peptidase and a peptidoglycan-mimetic boronate inhibitor: Interpretation of a transition state analogue in terms of catalytic mechanism

Biochemistry

Volumn 49, Issue 30, 2010, Pages 6411-6419

  Dzhekieva, Liudmila ^a   Rocaboy, Mathieu ^b   Kerff, Frédéric ^b   Charlier, Paulette ^b   Sauvage, Eric ^b   Pratt, R F ^a  

^a WESLEYAN UNIVERSITY   (United States)

^b UNIVERSITY OF LIÈGE   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ACTINOMADURA R39 DD-PEPTIDASE; ACTIVE SITE; AMINO TERMINUS; AMINOLYSIS; BACTERIAL CELLS; BORONATE; CATALYTIC MECHANISMS; COVALENTLY BOUND; DEACYLATION; HIGH ENERGY; HYDROXYL GROUPS; INHIBITORY COMPLEX; LEAVING GROUPS; LOW MOLECULAR WEIGHT; OXYANION HOLE; PENICILLIN- BINDING PROTEINS; PEPTIDE SUBSTRATES; PEPTIDOGLYCANS; POTENT INHIBITOR; SIDE CHAINS; TETRAHEDRAL INTERMEDIATES; TRANSITION-STATE ANALOGUES;

AMINATION; BACTERIOLOGY; BIOMIMETICS; CELL MEMBRANES; COMPLEXATION; OXYGEN; PEPTIDES; PROTON TRANSFER; QUANTUM CHEMISTRY; STEM CELLS;

CRYSTAL STRUCTURE;

(CHLOROMETHYL) BORONIC ACID PINANEDIOL ESTER; (DEXTRO ALPHA AMINOPIMELYLAMINO) DEXTRO 1 ETHYLBORONIC ACID; (DEXTRO ALPHA AMINOPIMELYLAMINO) DEXTRO 1 ETHYLBORONIC ACID PINANEDIOL ESTER; ANTIBIOTIC AGENT; BACTERIAL ENZYME; BORONIC ACID DERIVATIVE; DEXTRO 1 (AMINOETHYL) BORONIC ACID PINANEDIOL ESTER HYDROCHLORIDE; DEXTRO 1 [6 (N,N DIBENZYLAMINO) 6 (BENZYLOXYCARBONYL) TRANSHEX 2 ENOYLAMINO]ETHYLBORONIC ACID PINANEDIOL ESTER; DEXTRO 4 (N,N DIBENZYLAMINO) 4 (BENZYLOXYCARBONYL) BUTANAL; DEXTRO 6 (N,N DIBENZYLAMINO) 6 (BENZYLOXYCARBONYL) TRANS HEX 2 ENOIC ACID; DEXTRO 6 (N,N DIBENZYLAMINO) 6 (BENZYLOXYCARBONYL) TRANS HEX 2 ENOIC ACID TERT BUTYL ESTER; DEXTRO DEXTRO PEPTIDASE; ENZYME INHIBITOR; HYDROXYL GROUP; METHYLBORONIC ACID PINANEDIOL ESTER; N,N DIBENZYL DEXTRO GLUTAMIC ACID DIBENZYL ESTER; NUCLEOPHILE; OXYGEN; PEPTIDASE; PEPTIDOGLYCAN; UNCLASSIFIED DRUG;

ACTINOMADURA; ACYLATION; ARTICLE; BINDING COMPETITION; CATALYSIS; COMPETITIVE INHIBITION; COVALENT BOND; CRYSTAL STRUCTURE; DEACYLATION; DRUG STRUCTURE; DRUG SYNTHESIS; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT;

ACTINOMYCETALES; BACTERIAL PROTEINS; BORONIC ACIDS; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; ENZYME INHIBITORS; HYDROGEN BONDING; KINETICS; MOLECULAR MIMICRY; PENICILLIN-BINDING PROTEINS; PEPTIDOGLYCAN; PROTONS; SERINE-TYPE D-ALA-D-ALA CARBOXYPEPTIDASE; STRUCTURE-ACTIVITY RELATIONSHIP;

ACTINOMADURA; BACTERIA (MICROORGANISMS);

EID: 77955041712     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi100757c     Document Type: Article

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