Intraparticle concentration gradients for substrate and acidic product in immobilized cephalosporin C amidase and their dependencies on carrier characteristics and reaction parameters

Biotechnology and Bioengineering

Volumn 106, Issue 4, 2010, Pages 528-540

  Boniello, Caterina ^a   Mayr, Torsten ^b   Klimant, Ingo ^a,b   Koenig, Burghard ^a,c   Riethorst, Waander ^a,c   Nidetzky, Bernd ^a,b  

^a RESEARCH CENTRE APPLIED BIOCATALYSIS   (Austria)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^c NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Cephalosporin C; Immobilization; Mass transfer; pH gradients; Porous carrier

Indexed keywords

AMIDASE; CEPHALOSPORIN C; CEPHALOSPORIN C AMIDASE; CONCENTRATION GRADIENTS; EFFECTIVENESS FACTOR; ENZYME LOADING; FLUORESCENCE INTENSITIES; FREQUENCY DOMAINS; GEOMETRICAL PARAMETERS; IMMOBILIZED ENZYME; INTERNAL PH; INTRAPARTICLE; LIFETIME MEASUREMENTS; LUMINESCENCE LIFETIME; PARTICLE SUSPENSIONS; PH DEPENDENCE; PH GRADIENTS; PH SENSITIVE; PHENANTROLINES; PORE DIAMETERS; PROTEIN LOADINGS; RATE KINETICS; REACTION PARAMETERS; SEPABEADS; SPECIFIC ACTIVITY; STEADY STATE; SUBSTRATE CONCENTRATIONS; THIELE MODULUS;

CARRIER CONCENTRATION; ENZYMES; HYDROLYSIS; LUMINESCENCE; MASS TRANSFER; PARTICLE SIZE; SIGNAL TO NOISE RATIO;

SUSPENSIONS (FLUIDS);

AMIDASE; CEPHALOSPORIN C AMIDASE; FLUORESCEIN; IMMOBILIZED ENZYME; PHENANTHROLINE DERIVATIVE; RUTHENIUM DERIVATIVE; UNCLASSIFIED DRUG;

ACIDITY; ARTICLE; BIOCATALYST; CONCENTRATION RESPONSE; CONTROLLED STUDY; COVALENT BOND; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; HYDROLYSIS; LUMINESCENCE; MEASUREMENT; PARTICLE SIZE; PHOSPHORESCENCE; POROSITY; SIGNAL NOISE RATIO; STEADY STATE;

AMIDOHYDROLASES; CEPHALOSPORINS; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; KINETICS; LUMINESCENT MEASUREMENTS; MICROSPHERES; STAINING AND LABELING; TEMPERATURE;

EID: 77954291511     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22694     Document Type: Article

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