Deacylation activity of cephalosporin acylase to cephalosporin C is improved by changing the side-chain conformations of active-site residues

Biochemical and Biophysical Research Communications

Volumn 310, Issue 1, 2003, Pages 19-27

  Oh, Bora ^a   Kim, Myungsook ^a   Yoon, Jongchul ^a   Chung, Kyungwha ^b   Shin, Yongchul ^b   Lee, Dongsoon ^a   Kim, Youngsoo ^a  

^a Seoul National University   (South Korea)

^b Amicogen Inc   (South Korea)

Author keywords

7 Aminocephalosporanic acid; Cephalosporin acylase; Glutaryl 7 aminocephalosporanic acid; Penicillin acylase; Protein engineering; Substrate specificity

Indexed keywords

2 AMINOADIPIC ACID; AMIDASE; CEPHALOSPORIN ACYLASE; CEPHALOSPORIN C; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; DEACYLATION; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEREOSPECIFICITY;

EID: 0141514470     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2003.08.110     Document Type: Article

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