The 2.0 Å crystal structure of cephalosporin acylase

Structure

Volumn 8, Issue 10, 2000, Pages 1059-1068

  Kim, Youngsoo ^a,c   Yoon, Ki Hong ^b   Khang, Yongho ^a   Turley, Stewart ^c   Hol, Wim G J ^c  

^a Yeungnam University   (South Korea)

^b Woosong University   (South Korea)

^c UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Cephalosporin acylase; Cephalosporin C acylase; Glutaryl 7ACA acylase; Ntn hydrolase

Indexed keywords

7 AMINOCEPHALOSPORANIC ACID; BACTERIAL ENZYME; CEFALOTIN; CEPHALOSPORIN ACYLASE; CEPHALOSPORIN DERIVATIVE; HYDROLASE; PENICILLIN AMIDASE; PENICILLIN G; UNCLASSIFIED DRUG; CEPHALOSPORIN C; RECOMBINANT PROTEIN;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; BREVUNDIMONAS DIMINUTA; CRYSTAL STRUCTURE; DRUG STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; ENZYME SUBUNIT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; METABOLISM; MOLECULAR GENETICS; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; PSEUDOMONAS; SEQUENCE ALIGNMENT; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BINDING SITES; CEPHALOSPORINS; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PENICILLIN AMIDASE; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

EID: 0034435442     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(00)00505-0     Document Type: Article

References (39)

1. Global pharmaceutical market growth accelerates to 7 percent in 1998
2. Preparative methods for 7-aminocephalosporanic acid and 6-aminopenicillanic acid
3. Molecular cloning and structure of the gene for 7β-(4-carboxybutanamido)cephalosporanic acid acylase from a Pseudomonas strain
4. Biochemical characterization of a glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain BL072
5. Cloning and characterization of the genes for two distinct cephalosporin acylases from Pseudomonas strain
6. Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylase
7. High-level production, chemical modification and site-directed mutagenesis of cephalosporin acylase from Pseudomonas strain N176
8. Oxidative modification of a cephalosporin C acylase from Pseudomonas strain N176 and site-directed mutagenesis of the gene
9. In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130
10. Cephalosporin acylases
11. Molecular aspects of penicillin and cephalosporin acylases
12. A protein catalytic frame work with an N-terminal nucleophile is capable of self-activation
13. Structural classification of proteins: New superfamilies
14. Scop: A structural classification of protein database for the investigation of sequences and structures
15. Crystal structure of penicillin G acylase from the Bro1 mutant strain of Providencia rettgeri
16. Isolation of novel Pseudomonas diminuta KAC-1 strain producing glutaryl 7-Aminocephalosporanic acid acylase
17. Phase determination from multiwavelength anomalous diffraction measurements
18. Penicillin acylase has a single-amino acid catalytic centre
19. Distribution and substrate specificity of benzylpenicillin acylase
20. Experimental evolution of penicillin G acylase from Escherichia coli and Proteus rettgeri
21. A novel 7β-(4-carboxybutanamido)-cephalosporanic acid isolated from Pseudomonas strain C427 and its high-level production in Escherichia coli
22. BCM search launcher-an integrated interface to molecular biological data base search and analysis services available on the world wide web
23. Protein structure comparison by alignment of distance matrices
24. Cloning and nucleotide sequencing of new glutaryl 7-ACA and Cephalosporin C acylase genes from Pseudomonas strain
25. Penicillin V acylase crystal structure reveals new Ntn-hydrolase family members
26. Crystallization and preliminary X-ray analysis of caphalosporin acylase from Pseudomonas sp. Strain N176
27. Preparation of selenomethionyl proteins for phase determination
28. Processing of X-ray diffraction data collected in oscillation mode
29. Automated MAD and MIR structure solution
30. Maximum-likelihood heavy-atom parameter refinement from multiple isomorphous replacement and multiwavelength anomalous diffraction methods
31. Methods used in the structure determination of bovine mitochondrial F-1 ATPase
32. Improved methods for building protein models in electron density maps and the location of errors in these models
33. Crystallography and NMR system: A new software suite for macromolecular structure determination
34. PROCHECK - A program to check the stereochemical quality of protein structures
35. Analysis of the steric strain in the polypeptide backbone of protein molecules
36. Molscript: A program to produce both detailed and schematic plots of protein structures
37. An extensively modified version of Molscript that includes greatly enhanced coloring capabilities
38. Raster3D: Photorealistic molecular graphics