Epoxy Sepabeads: A novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment

Biotechnology Progress

Volumn 18, Issue 3, 2002, Pages 629-634

  Mateo, Cesar ^a   Abian, Olga ^a   Fernández Lorente, Gloria ^a   Pedroche, Justo ^a   Fernández Lafuente, Roberto ^a   Guisan, Jose M ^a   Tam, Andrea ^a   Daminati, Moreno ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

EPOXIDE; IMMOBILIZED ENZYME; PENICILLIN AMIDASE;

ARTICLE; CHEMISTRY; ENZYME STABILITY; METABOLISM; SURFACE PROPERTY;

ENZYME STABILITY; ENZYMES, IMMOBILIZED; EPOXY COMPOUNDS; PENICILLIN AMIDASE; SURFACE PROPERTIES;

AMINO ACIDS; ENZYME IMMOBILIZATION; EPOXY RESINS; HYDROPHILICITY; HYDROPHOBICITY;

CYLINDRICAL PORES;

ENZYMES;

EID: 0035988098     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp010171n     Document Type: Article

References (30)

1. Immobilization of enzymes and cells
2. Biocatalysis: Immobilized cells and enzymes
3. Thermostabilization of proteins
4. Immobilized biocatalysts - From simple to complex systems
5. Immobilized enzymes - Learning from past successes and failures
6. Immobilized enzymes and cells
7. Immobilized enzymes and cells as practical catalysts
8. Immobilized biocatalysts - A critical review
9. Immobilized enzymes as catalyst
10. Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment
11. One step purification, covalent immobilization and additional satabilization of poly-hys-tagged proteins using a novel heterofunctional chelate-epoxy supports
12. Salt-mediated retention of proteins in hydrophobic-interaction chromatography. Application of solvophobic theory
13. Influence of salts on the covalent immobilization of proteins to modified copolymers of 2-hydroxiethylmetacrylate with ethilene dimetacrylate
14. Salt induced immobilization of proteins on a high-performans liquid chromatographic epoxide afinity support
15. Salt induced immobilization of afinity ligands onto epoxide activated supports
16. Oxirane acrylic beads for protein immobilization: A novel matrix for biocatalysis and biospecific adsorption
17. Selective enzymatic oxidations: Stabilization by multipoint covalent attachment of ferredoxin NAD-reductase: An interesting cofactor recycling enzyme
18. The coimmobilization of D-amino acid oxidase and catalase enables the quantitative transformation of D-amino acids (D-phenylalanine) into keto acids (phenylpyruvic acid)
19. Stabilization of enzymes against thermal inactivation
20. Enzyme stabilization: State of the art
21. Immobilization and properties of Bacillus stearothermophilus pulanase
22. Preparation and properties of glucose isomerase immobilized on indon 48-R
23. Immobilized nucleases
24. Aldehyde gels as activated support for immobilization-stabilization of enzymes
25. Immobilization-stabilization of chymotrypsin by covalent attachment to aldehyde agarose gels
26. Operational stability of copolymerized enzymes at elevated temperatures
27. Modification of enzyme properties by the use of inhibitors during their stabilization by multipoint covalent attachment
28. Immobilization-stabilization of enzymes. Variables that control the intensity of the trypsin (amine)-agarose (aldehyde) multi-point covalent attachment
29. Immobilization-stabilization of penicillin G acylase from E. coli
30. Multifunctional epoxy-supports: A new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage