Optimization of an industrial biocatalyst of glutaryl acylase: Stabilization of the enzyme by multipoint covalent attachment onto new amino-epoxy Sepabeads

Journal of Biotechnology

Volumn 111, Issue 2, 2004, Pages 219-227

  López Gallego, Fernándo ^a   Betancor, Lorena ^a   Hidalgo, Aurelio ^a   Mateo, Cesar ^a   Guisán, Jose M ^a   Fernández Lafuente, Roberto ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ENZYME IMMOBILIZATION; LOW TEMPERATURE EFFECTS; OPTIMIZATION; PH; SOLUBILITY;

HALF-LIVES; STABILIZING EFFECTS;

ENZYMES;

AGAROSE; AMIDASE; GLUTARYL ACYLASE; UNCLASSIFIED DRUG;

ARTICLE; BIOCATALYST; COVALENT BOND; ENZYME IMMOBILIZATION; ENZYME STABILITY; HALF LIFE TIME; INCUBATION TIME; LOW TEMPERATURE; PH; PRIORITY JOURNAL; THERMOSTABILITY;

ADSORPTION; CATALYSIS; CHEMICAL INDUSTRY; COATED MATERIALS, BIOCOMPATIBLE; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; EPOXY RESINS; HYDROGEN-ION CONCENTRATION; KINETICS; MATERIALS TESTING; MICROSPHERES; PENICILLIN AMIDASE; PROTEIN BINDING; TEMPERATURE;

EID: 3042655014     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2004.04.006     Document Type: Article

References (30)

1. Alvaro G., Fernández-Lafuente R., Blanco R.M., Guisán J.M. Immobilization-stabilization of penicillin G acylase from Escherichia coli. Appl. Biochem. Biotech. 26:1990;181-195
2. Aramori I., Fukagawa M., Tsumura M., Iwami M., Isogai T., Ono H., Ishitani Y., Kojo H., Kohsaka M., Ueda Y., Imanaka H. Isolation of soil strains producing new cephalosporin acylases. J. Ferment. Bioeng. 72:1991;232-243
3. Balcão V.M., Mateo C., Fernández-Lafuente R., Malcata F.V., Guisán J.M. Structural and functional stabilization of L-asparaginase via multisubunit immobilization onto highly activated supports. Biotechnol. Prog. 17:2001;537-542
4. Batista-Viera F., Brena B., Luna B. Reversible immobilization of soybean amylase on phenylboronate-agarose. Biotechnol. Bioeng. 31:1988;711-713
5. Bianchi D., Golini P., Bortolo R., Battistel E., Tassainari R., Cesti P. Immobilization of glutaryl-7-ACA acylase on aminoalkylated polyacrylic supports. Enzyme Microb. Technol. 20:1997;368-372
6. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 76:1976;248
7. Fechtig B., Peter H., Bickel H., Visher E. Concerning the preparation of 7-amino-cephalosporanic acid. Helv. Chim. Act. 51:1968;1109-1120
8. Fernández-Lafuente R., Rosell C.M., Rodriguez V., Santana C., Soler G., Bastida A., Guisán J.M. Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method. Enzyme Microb. Technol. 15:1993;546-550
9. Gianfreda L., Scarfi M.R. Enzyme stabilization: state of the art. Mol. Cell. Biochem. 100:1991;97-128
10. Guisán J.M. Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes. Enzyme Microb. Technol. 10:1988;375-382
11. Gupta M.N. Thermostabilization of proteins. Biotechnol. Appl. Biochem. 14:1991;1-11
12. Hernández-Justiz O., Fenández-Lafuente R., Guisán J.M., Negri P., Pagani G., Pregnolato M., Terreni M. One-pot chemoenzymatic synthesis of 3′-functionalized cephalosporines (Cefazolin) by three consecutive biotransformations in fully aqueous medium. J. Org. Chem. 62:1997;9099-9106
13. Klibanov A.M. Stabilization of enzymes against thermal inactivation. Adv. Appl. Microb. 29:1982;1-28
14. Martinek K., Klibanov A.M., Glodmacher V.S., Berezin I.V. The principles of enzyme stabilization. I. Increase in thermostability of enzymes covalently bound to a complementary surface of a polymer support in a multipoint fashion. Biochem. Biophys. Acta. 485:1977;13-28
15. Mateo C., Fernández-Lorente G., Abian O., Fernández- Lafuente R., Guisán J.M. Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage. Biomacromolecules. 1:2000;739-745
16. Mateo C., Torres R., Fernández-Lorente G., Ortiz C., Fuentes M., Hidalgo A., López-Gallego F., Abian O., Palomo J.M., Betancor L., Pessela B.C.C., Guisán J.M., Fernández-Lafuente R. Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method. Biomacromolecules. 4:2003;772-777
17. Mateo C., Abian O., Fernández-Lorente G., Pedroche J., Fernández-Lafuente R., Guisan J.M., Tam A., Daminati M. Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment. Biotech. Progr. 18:2002;629-634
18. Melander W., Corradini D., Hoorvath C. Salt-mediated retention of proteins in hydrophobic-interaction chromatography: application of solvophobic theory. J. Chromatogr. 317:1984;67-85
19. Monti D., Carrea G., Riva S., Baldaro E., Frare G. Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase. Biotech. Bioeng. 70:2000;239
20. Nikolov A., Danielsson B. Enzymatic transformation of cephalosporin C to 7-amino-cephalosporanic acid. Part I: Cultivation of Pseudomonas syringae and partial purification and immobilization of 7-(4-carboxybutanamido) cephalosporanic acid acylase. Enzyme Microb. Technol. 16:1994a;1031-1036
21. Nikolov A., Danielsson B. Enzymatic transformation of cephalosporin C to 7-amino-cephalosporanic acid. Part II: Single-step procedure using a coimmobilized enzyme system. Enzyme Microb. Technol. 16:1994b;1037-1041
22. Park S.W., Choi S.Y., Chung K.H., Hong S.I., Kim S.W. New method for the immobilization of glutaryl-7-aminocephalosporanic acid acylase on a modified epoxy/silica gel hybrid. J. Biosci. Bioeng. 94:2002;218-224
23. Park S.W., Kim Y.I., Chung K.H., Kim S.W. Improvement of stability of immobilized GL-7-ACA acylase through modification with glutaraldehyde. Process Biochem. 37:2001;153-163
24. Pessela B.C.C., Mateo C., Carrascosa A.V., Vian A., García J.L., Guisan J.M., Fernández-Lafuente R. One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-His-tagged beta-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate-epoxy Sepabeads. Biomacromolecules. 4:2003;107-113
25. Schroën C.G., VandeWiel S., Kroon P.J., DeVroom E., Janssen A.E., Tramper J. Equilibrium position, kinetics, and reactor concepts for the adipyl-7-ADCA-hydrolysis process. Biotech. Bioeng. 70:2000;651-654
26. Schussel L.J., Atwater J.E. A continuous alcohol oxidase bioreactor for regenerative life support. Enzyme Microb. Technol. 18:1996;229-235
27. Smalla K., Turkova J., Coupek J., Herman P. Influence of salts on the covalent immobilization of proteins to modified copolymers of 2-hydroxyethyl methacrylate with ethylene dimethacrylate. Biotechnol. Appl. Biochem. 10:1988;21-31
28. Terreni M., Pagani G., Ubiali D., Fernández-Lafuente R., Mateo C., Guisán J.M. Modulation of penicillin acylase properties via immobilization techniques: one-pot chemoenzymatic synthesis of cephamandole from cephalosporin. Bioorg. Med. Chem. Lett. 18:2001;2429-2432
29. Tischer W., Wedekind F. Immobilized enzymes: methods and applications. Top. Curr. Chem. 200:1999;95-126
30. Ulbrich-Hofmann R., Arnold U., Mansfeld J. The concept of the unfolding region for approaching the mechanism of enzyme stabilization. J. Mol. Catal. 7:1999;125-131