Direct measurement of pH profiles in immobilized enzyme carriers during kinetically controlled synthesis using CLSM

Biotechnology Progress

Volumn 17, Issue 2, 2001, Pages 294-303

  Spiess, A C ^a   Kasche, V ^a  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

IMMOBILIZED ENZYMES;

BIOTECHNOLOGICAL PROCEDURES; CEPHALOSPORIN; CONFOCAL LASER MICROSCOPY; ENZYME SYNTHESIS; IMMOBILIZED ENZYME; PARTICLE; PENICILLIN AMIDASE; PENICILLIN; PH;

BIOSYNTHESIS; PARTICLE SIZE ANALYSIS; PH EFFECTS; POROSITY;

ENZYMES;

CALIBRATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYMES, IMMOBILIZED; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; KINETICS; LASERS; MICROSCOPY, CONFOCAL;

EID: 0034919698     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000149e     Document Type: Article

References (32)

1. Immobilized α-chymotrypsin: Pore diffusion control owing to pH gradients in the catalyst particles
2. Generation of a pH gradient in an immobilized enzyme system
3. pH gradients in immobilized amidases and their influence on rates and yields of β-lactam hydrolysis
4. Fluorescence ratio imaging: Issues and artifacts
5. Influence of cytosolic pH changes on the organization of the endoplasmic reticulum in epidermal cells of onion bulb scales: Acidification by loading with weak organic acids
6. Measurement of intracellular pH with a laser scanning confocal microscope
7. Confocal microscopic images of a compost particle
8. Relationship between mass transfer coefficient and liquid flow velocity in heterogeneous biofilms using microelectrodes and confocal microscopy
9. Investigation of single protein adsorption on ion exchangers using confocal laser scanning microscopy
10. Enzymatic synthesis of β-lactam antibiotics: A thermodynamic background
11. Thermodynamics of the conversion of penicillin G to phenylacetic acid and 6-aminopenicillanic acid
12. Feasibility of the thermodynamically controlled synthesis of amoxicillin
13. Mechanism and yields in enzyme catalysed equilibrium and kinetically controlled synthesis of β-lactam antibiotics, peptides and other condensation products
14. Correlation of experimental and theoretical data for artificial and natural systems with immobilized biocatalysts
15. Repression of penicillin G acylase of Proteus rettgeri by tricarboxylic acid cycle intermediates
16. Direct spectrophotometric measurement of enzyme activity in heterogeneous systems with insoluble substrate or immobilized enzyme
17. Poröses glas als enzymträger
18. Measurement of electrical potential, pH, and free calcium ion concentration in mitochondria of living cells by laser scanning confocal microscopy
19. Flow distribution in packed beds