Evaluation of the performance of immobilized penicillin G acylase using active-site titration

Biotechnology and Bioengineering

Volumn 78, Issue 4, 2002, Pages 425-432

  Janssen, Michiel H A ^a   Van Langen, Luuk M ^a   Pereira, Silvia R M ^a   Van Rantwijk, Fred ^a   Sheldon, Roger A ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

Active site titration; Cephalexin synthesis; Eupergit C; Immobilization; Penicillin acylase; S H ratio

Indexed keywords

DIFFUSION; DRUG PRODUCTS; ESCHERICHIA COLI; HYDROLYSIS; MOLECULES; SUBSTRATES; SYNTHESIS (CHEMICAL); TITRATION;

ENZYME LOADING;

ENZYME IMMOBILIZATION;

AMIDASE; CEFALEXIN; PENICILLIN ACYLASE; PENICILLIN G; PHENYLGLYCINE; UNCLASSIFIED DRUG;

ARTICLE; DIFFUSION COEFFICIENT; DRUG SYNTHESIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME METABOLISM; ESCHERICHIA COLI;

ANTIGENS, NEOPLASM; CATALYSIS; CELL ADHESION MOLECULES; DIFFUSION; ENZYMES, IMMOBILIZED; HYDROLYSIS; MEMBRANE GLYCOPROTEINS; MODELS, CHEMICAL; PENICILLIN AMIDASE; PENICILLINS; PHENYLMETHYLSULFONYL FLUORIDE; POLYMERS; SENSITIVITY AND SPECIFICITY; TITRIMETRY;

ESCHERICHIA COLI;

EID: 0037141606     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10208     Document Type: Article

References (17)

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