Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C

Protein Science

Volumn 11, Issue 1, 2002, Pages 92-103

  Fritz Wolf, Karin ^a   Koller, Klaus Peter ^b   Lange, Gudrun ^b   Liesum, Alexander ^b   Sauber, Klaus ^b   Schreuder, Herman ^b   Aretz, Werner ^b   Kabsch, Wolfgang ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^b SANOFI AVENTIS DEUTSCHLAND GMBH   (Germany)

Author keywords

Catalytic triad; Cephalosporin acylase; Cephalosporin C; Glutaryl acylase; Ntn hydrolase; X ray structure

Indexed keywords

7 AMINOCEPHALOSPORANIC ACID; AMIDASE; CEPHALOSPORIN C; GLUTARYLAMIDASE; PENICILLIN G; SERINE PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CRYSTALLIZATION; DRUG SYNTHESIS; ENZYME ANALYSIS; ENZYME MODIFICATION; ENZYME STRUCTURE; MUTATION; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; CATALYSIS; CEPHALOSPORINS; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PENICILLIN AMIDASE; PROTEIN BINDING;

EID: 0036137006     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.ps.27502     Document Type: Article

References (33)

1. Methods used in the structure determination of bovine mitochondrial F-1 ATPase
2. Cloning and nucleotide sequencing of new Glutaryl 7-ACA and cephalosporin C acylase genes from Pseudomonas strain
3. Enzymatische herstellung von 7-aminocephalosporansäure
4. A protein catalytic framework with an N-terminal nucleophile is capable of self-activation
5. Crystallography & NMR system (CNS): A new software system for macromolecular structure determination
6. High-level incorporation of methionine in proteins by its analogs 2-aminohexanoic acid, selenomethionine, telluromethionine and ethionine in Escherichia coli
7. Low-barrier hydrogen bonds and enzymatic catalysis
8. Hydrolysis of penicillins and related compounds by the cell-bound penicillin acylase of E. coli
9. Improved R-factors for diffraction data analysis in macromolecular crystallography
10. Penicillin acylase has a single-amino-acid catalytic center
11. An extensively modified version of MolScript that includes greatly enhanced coloring capabilities
12. Analysis of the steric strain in the polypeptide backbone of protein molecules
13. Distribution and substrate specificity of benzylpenicillin acylase
14. Punfication and properties of 7β-(4-carboxybutanamido-)cephalosporanic acid acylase produced by mutants derived from Pseudomonas
15. High-level production chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176
16. Improved methods of building protein models in electron density maps and the location of errors in these models
17. A discussion of the solution for the best rotation to relate two sets of vectors
18. Evaluation of single-crystal X-ray diffraction data from a position-sensitive detector
19. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants
20. The 2.0 Å crystal structure of cephalosporin acylase
21. Molscript: A program to produce both detailed and schematic plots of protein structures
22. Procheck: A program to check the stereochemical quality of protein structures
23. Two-step autocatalytic processing of the glutaryl 7-aminocephalosporanic acid acylase from Pseudomonas sp. strain GK16
24. Refined atomic model of wheat serine carboxypeptidase II at 2.2 Å resolution
25. Molecular cloning and structure of the gene for 7β-(4-carboxybutanamido)cephalosporanic acid acylase from a Pseudomonas strain
26. Crystal structure of penicillin G acylase from the Brol mutant strain of Providencia rettgeri
27. Raster3D: Photorealistic molecular graphics
28. Chemical modification and site-directed mutagenesis of tyrosines residues in Cephalosporin C acylase from strain N176
29. Catalytic hydroxyl/amine dyads within serine proteases
30. Isolation and properties of 7β(4-carboxybutanamido)-cephalosporanic acid acylase producing bacteria
31. A family of protein-cutting proteins