A highly active adipyl-cephalosporin acylase obtained via rational randomization

FEBS Journal

Volumn 274, Issue 21, 2007, Pages 5600-5610

  Otten, Linda G ^b,d   Sio, Charles F ^c,d   Reis, Carlos R ^d   Koch, Gudrun ^d   Cool, Robbert H ^d   Quax, Wim J ^a,d  

^a Pharmaceutical Biology   (Netherlands)

^b DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^c PHILIPS RESEARCH LABORATORIES   (Netherlands)

^d UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

7 aminocephalosporanic acid; Biocatalysis; Cephalosporin acylase; Directed evolution; Protein engineering

Indexed keywords

7 AMINOCEPHALOSPORANIC ACID; 7 AMINODESACETOXYCEPHALOSPORANIC ACID; AMIDASE; CEPHALOSPORANIC ACID DERIVATIVE; CEPHALOSPORIN C; CEPHALOSPORIN DERIVATIVE; LEUCINE; MUTANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM MUTANT; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE MUTATION; HYDROLYSIS; MOLECULAR EVOLUTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PSEUDOMONAS; RANDOMIZATION;

BINDING SITES; CATALYSIS; CEPHALOSPORINS; GENETIC ENGINEERING; MODELS, MOLECULAR; MUTAGENESIS; MUTATION; PENICILLIN AMIDASE; PSEUDOMONAS; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; PSEUDOMONAS;

EID: 35449007305     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.06081.x     Document Type: Article

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