α-Bisabolol induces dose- and time-dependent apoptosis in HepG2 cells via a Fas- and mitochondrial-related pathway, involves p53 and NFκB

Biochemical Pharmacology

Volumn 80, Issue 2, 2010, Pages 247-254

  Chen, Wei ^a   Hou, Jie ^a   Yin, Yan ^a   Jang, Jongchol ^a   Zheng, Zhongliang ^a   Fan, Handong ^b   Zou, Guolin ^a  

^a WUHAN UNIVERSITY   (China)

^b INSTITUTE OF APPLIED CHEMISTRY   (China)

Author keywords

Bisabolol; Apoptosis; Extrinsic; HepG2; Intrinsic

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BISABOLOL; CASPASE; CASPASE 3; CASPASE 8; CASPASE 9; FAS ANTIGEN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PROTEIN BAK; PROTEIN BAX; PROTEIN BCL 2; PROTEIN BID; PROTEIN P53; SESQUITERPENE;

APOPTOSIS; ARTICLE; BIOACCUMULATION; CARCINOMA CELL; CELL ASSAY; CELL NUCLEUS; CELL STRAIN HEPG2; CHROMOSOME; CONCENTRATION RESPONSE; CYTOSOL; DNA FRAGMENTATION; DOWN REGULATION; DRUG CYTOTOXICITY; DRUG STRUCTURE; ENZYME ACTIVATION; FLOW CYTOMETRY; GENETIC TRANSCRIPTION; HELA CELL; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LIVER CARCINOMA; MITOCHONDRION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ANTIGENS, CD95; ANTINEOPLASTIC AGENTS; APOPTOSIS; CELL CYCLE; CELL LINE, TUMOR; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG SCREENING ASSAYS, ANTITUMOR; FEMALE; FORMAZANS; HEP G2 CELLS; HUMANS; MALE; MITOCHONDRIA, LIVER; NF-KAPPA B; POLY(ADP-RIBOSE) POLYMERASES; SESQUITERPENES; TETRAZOLIUM SALTS; TIME FACTORS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 77952671540     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bcp.2010.03.021     Document Type: Article

References (54)

1. Bruix J., Boix L., Sala M., Llovet J.M. Focus on hepatocellular carcinoma. Cancer Cell 2004, 5:215-219.
2. Bosch F.X., Ribes J., Borras J. Epidemiology of primary liver cancer. Semin Liver Dis 1999, 19:271-285.
3. Hengartner M.O. The biochemistry of apoptosis. Nature 2000, 407:770-776.
4. Meier P., Vousden K.H. Lucifer's labyrinth-ten years of path finding in cell death. Mol Cell 2007, 28:746-754.
5. Ashkenazi A. Death receptors: signaling and modulation. Science 1998, 281:1305-1308.
6. Peter M.E. Mechanisms of CD95 (APO-1/Fas)-mediated apoptosis. Curr Opin Immunol 1998, 10:545-551.
7. Reed J.C. Mechanisms of apoptosis. Am J Pathol 2000, 157:1415-1430.
8. Kuwana T., Newmeyer D.D. Bcl-2-family proteins and the role of mitochondria in apoptosis. Curr Opin Cell Biol 2003, 15:691-699.
9. Chipuk J.E., Green D.R. How do BCL-2 proteins induce mitochondrial outer membrane permeabilization?. Trends Cell Biol 2008, 18:157-164.
10. Schafer B., Quispe J., Choudhary V., Chipuk J.E., Ajero T.G., Du H., et al. Mitochondrial outer membrane proteins assist Bid in Bax-mediated lipidic pore formation. Mol Biol Cell 2009, 20:2276-2285.
11. Dave Z., Byfield M., Bossy-Wetzel E. Assessing mitochondrial outer membrane permeabilization during apoptosis. Methods 2008, 46:319-323.
12. Kroemer G., Reed J.C. Mitochondrial control of cell death. Nat Med 2000, 6:513-519.
13. Rivero A., Quintana J., Eiroa J.L., Lopez M., Triana J., Bermejo J., et al. Potent induction of apoptosis by germacranolide sesquiterpene lactones on human myeloid leukemia cells. Eur J Pharmacol 2003, 482:77-84.
14. Chen C.N., Huang H.H., Wu C.L., Lin C.P.C., Hsu J.T.A., Hsieh H.P., et al. Isocostunolide, a sesquiterpene lactone, induces mitochondrial membrane depolarization and caspase-dependent apoptosis in human melanoma cells. Cancer Lett 2007, 246:237-252.
15. Rozenblat S. Induction of G2/M arrest and apoptosis by sesquiterpene lactones in human melanoma cell lines. Biochem Pharmacol 2008, 75:369-382.
16. Bhatia S.P. Fragrance material review on α-bisabolol. Food Chem Toxicol 2008, 46:572-576.
17. Brehm-Stecher B.F. Sensitization of Staphylococcus aureus and Escherichia coli to antibiotics by the sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone. Antimicrob Agents Chemother 2003, 47:3357-3360.
18. Gomes-Carneiro M.R. Evaluation of mutagenic and antimutagenic activities of α-bisabolol in the Salmonella/microsome assay. Mutat Res 2005, 585:105-112.
19. Alvarez-Gonzalez I., Uc-Artigas E., Moreno M., Madrigal-Bujaidar E. Inhibitory effect of alpha-bisabolol on the genotoxic damage induced by daunorubicin in mouse. Toxicol Lett 2006, 164:S268-S1268.
20. Kim S., Lee J., Jung E., Huh S., Park J.O., Lee J.W., et al. Mechanisms of depigmentation by alpha-bisabolol. J Dermatol Sci 2008, 52:219-222.
21. Cavalieri E., Mariotto S., Fabrizi C., de Prati A.C., Gottardo R., Leone S., et al. Alpha-bisabolol, a nontoxic natural compound, strongly induces apoptosis in glioma cells. Biochem Biophys Res Commun 2004, 315:589-594.
22. Darra E., Abdel-Azeim S., Manara A., Shoji K., Mareechal J.D., Mariotto S., et al. Insight into the apoptosis-inducing action of alpha-bisabolol towards malignant tumor cells: involvement of lipid rafts and Bid. Arch Biochem Biophys 2008, 476:113-123.
23. Cavalieri E., Bergamini C., Mariotto S., Leoni S., Perbellini L., Darra E., et al. Involvement of mitochondrial permeability transition pore opening in alpha-bisabolol induced apoptosis. FEBS J 2009, 276:3990-4000.
24. Zhou H. G1-phase specific apoptosis in liver carcinoma cell line induced by copper-1,10-phenanthroline. Int J Biochem Cell Biol 2002, 34:678-684.
25. Cai X.Q., Pan N., Zou G. Copper-1,10-phenanthroline-induced apoptosis in liver carcinoma Bel-7402 cells associates with copper overload, reactive oxygen species production, glutathione depletion and oxidative DNA damage. Biometals 2007, 20:1-11.
26. Herrmann M. A rapid and simple method for the isolation of apoptotic DNA fragments. Nucleic Acids Res 1994, 22:5506-5507.
27. Nicholson D. Caspases: killer proteases. Trends Biochem Sci 1997, 22:299-306.
28. Scaffidi C. Two CD95 (APO-1/Fas) signaling pathways. EMBO J 1998, 17:1675-1687.
29. Kischkel C. F. Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor. EMBO J 1995, 14:5579-5588.
30. Medema J.P. FLICE is activated by association with the CD95 death-inducing signaling complex (DISC). EMBO J 1997, 16:2794-2804.
31. Jung J.Y., Kim W.J. Involvement of mitochondrial- and Fas-mediated dual mechanism in CoCl2-induced apoptosis of rat PC12 cells. Neurosci Lett 2004, 371:85-90.
32. Gross A., McDonnell J.M., Korsmeyer S.J. BCL-2 family members and the mitochondria in apoptosis. Genes Dev 1999, 13:1899-1911.
33. Gross A. Enforced dimerization of BAX results in its translocation, mitochondrial dysfunction and apoptosis. EMBO J 1998, 17:3878-3885.
34. Sattler M. Structure of Bcl-xL-Bak peptide complex: recognition between regulators of apoptosis. Science 1997, 275:983-986.
35. Oh S.H., Lee B.H. A ginseng saponin metabolite-induced apoptosis in HepG2 cells involves a mitochondria-mediated pathway and its downstream caspase-8 activation and Bid cleavage. Toxicol Appl Pharmacol 2004, 194:221-229.
36. Oh S.H., Lee B.H. Induction of apoptosis in human hepatoblastoma cells by tetrandrine via caspase-dependent Bid cleavage and cytochrome c release. Biochem Pharmacol 2003, 66:725-731.
37. Kruse J.P., Gu W. Modes of p53 regulation. Cell 2009, 137:609-622.
38. Oda E. Noxa, a BH3-only member of the BcL-2 family and candidate mediator of p53-induced apoptosis. Science 2000, 288:1053-1058.
39. Leu J.I.J., Dumont P., Hafey M., Murphy M.E., George D.L. Mitochondrial p53 activates Bak and causes disruption of a Bak-Mcl1 complex. Nat Cell Biol 2004, 6:443-450.
40. Harris C.C. p53 Tumor suppressor gene: from the basic research laboratory to the clinic-an abridged historical perspective. Carcinogenesis 1996, 17:1187-1198.
41. Owen-Schaub L.B. Wild-type human p53 and a temperature-sensitive mutant induce Fas/APO-1 expression. Mol Cell Biol 1995, 15:3032-3040.
42. Petak I., Tillman D.M., Houghton J.A. P53 dependence of Fas induction and acute apoptosis in response to 5-fluorouracil-leucovorin in human colon carcinoma cell lines. Clin Cancer Res 2000, 6:4432-4441.
43. Monte SMdl. Correlates of p53- and Fas (CD95)-mediated apoptosis in Alzheimer's disease. J Neurol Sci 1997, 152:73-83.
44. Muller M. Drug-induced apoptosis in hepatoma cells is mediated by the CD95 (APO-1/Fas) receptor/ligand system and involves activation of wild-type p53. J Clin Invest 1997, 99:403-413.
45. Wang C.-Y. NF-kB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation. Science 1998, 281:1680-1683.
46. Micheau O., Lens S., Gaide O., Alevizopoulos K., Tschopp J. NF-kappa B signals induce the expression of c-FLIP. Mol Cell Biol 2001, 21:5299-5305.
47. Penolazzi L., Lambertini E., Borgatti M., Piva R., Cozzani M., Giovannini I., et al. Decoy oligodeoxynucleotides targeting NF-kappaB transcription factors: induction of apoptosis in human primary osteoclasts. Biochem Pharmacol 2003, 66:1189-1198.
48. Wahl C., Maier S., Marre R., Essig A. Chlamydia pneumoniae induces the expression of inhibitor of apoptosis 2 (c-IAP2) in a human monocytic cell line by an NF-kappaB-dependent pathway. Int J Med Microbiol 2003, 293:377-381.
49. Colgate E.C., Miranda C.L., Stevens J.F., Bray T.M., Ho E. Xanthohumol, a prenylflavonoid derived from hops induces apoptosis and inhibits NF-kappaB activation in prostate epithelial cells. Cancer Lett 2007, 246:201-209.
50. Dai Y., Liu M.L., Tang W.H., Li Y.M., Lian J.Q., Lawrence T.S., et al. A Smac-mimetic sensitizes prostate cancer cells to TRAIL-induced apoptosis via modulating both IAPs and NF-kappaB. BMC Cancer 2009, 9.
51. Ryan K.M., Ernst M.K., Rice N.R., Vousden K.H. Role of NF-kappa B in p53-mediated programmed cell death. Nature 2000, 404:892-897.
52. Webster G.A., Perkins N.D. Transcriptional cross talk between NF-kappa B and p53. Mol Cell Biol 1999, 19:3485-3495.
53. Tergaonkar V., Pando M., Vafa O., Wahl G., Verma I. P53 stabilization is decreased upon NF kappa B activation: a role for NF kappa B in acquisition of resistance to chemotherapy. Cancer Cell 2002, 1:493-503.
54. Marusawa H., Hijikata N., Watashi K., Chiba T., Shimotohno K. Regulation of Fas-mediated apoptosis by NF-kappa B activity in human hepatocyte derived cell lines. Microbiol Immunol 2001, 45:483-489.