Role of Enzyme-Ribofuranosyl contacts in the ground state and transition state for orotidine 5′-phosphate decarboxylase: A role for substrate destabilization?

Biochemistry

Volumn 40, Issue 21, 2001, Pages 6227-6232

  Miller, Brian G ^a   Butterfoss, Glenn L ^a   Short, Steven A ^b   Wolfenden, Richard ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROSTATIC REPULSION;

CATALYSIS; CRYSTAL STRUCTURE; FREE ENERGY; GROUND STATE; HYDROGEN BONDS; YEAST;

ENZYMES;

AZAURACIL; BARBITURIC ACID; FUNGAL ENZYME; MUTANT PROTEIN; OROTIC ACID; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; RIBOSE; URACIL; URIDINE PHOSPHATE; XANTHINE;

ARTICLE; BINDING AFFINITY; CRYSTAL STRUCTURE; DECARBOXYLATION; DISSOCIATION CONSTANT; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; YEAST;

ALANINE; ASPARTIC ACID; CATALYSIS; ENZYME STABILITY; KINETICS; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; RIBOSEMONOPHOSPHATES; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY; THREONINE; URIDINE MONOPHOSPHATE;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 0035967499     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0028993     Document Type: Article

References (16)

1. Jones, M. E. (1980) Annu. Rev. Biochem. 49, 253-279.
2. Radzicka, A., and Wolfenden, R. (1995) Science 267, 90-93.
3. Miller, B. G., Smiley, J. A., Short, S. A., and Wolfenden, R. (1999) J. Biol. Chem. 274, 23841-23843.
4. Miller, B. G., Hassell, A. M., Wolfenden, R., Milburn, M. V., and Short, S. A. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 2011-2016.
5. Wu, N., Mo, Y., Gao, J., and Pai, E. F. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 2017-2022.
6. Appleby, T. C., Kinsland, C., Begley, T. P., and Ealick, S. E. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 2005-2010.
7. Harris, P., Poulsen, J.-C. N., Jensen, K. F., and Larsen, S. (2000) Biochemistry 39, 4217-4224.
8. Miller, B. G., Snider, M. J., Short, S. A., and Wolfenden, R. (2000) Biochemistry 39, 8113-8118.
9. Miller, B. G., Traut, T. W., and Wolfenden, R. (1998) Bioorg. Chem. 26, 283-288.
10. Livingstone, L. R., and Jones, M. E. (1987) J. Biol. Chem. 262, 15726-15733.
11. Jones, M. E., Kavipurapa, P. R., and Traut, T. W. (1978) Methods Enzymol. 51, 155-157.
12. Edelhoch, H. (1967) Biochemistry 6, 1948-1954.
13. Wells, J. A. (1990) Biochemistry 29, 8509-8517.
14. Rishavy, M. A., and Cleland, W. W. (2000) Biochemistry 39, 4569-4574.
15. Porter, D. J. T., and Short, S. A. (2000) Biochemistry 39, 11788-11800.
16. Miller, B. G., Snider, M. J., Wolfenden, R., and Short, S. A. (2001) J. Biol. Chem. (in press).