The effect of marathon on mRNA expression of anti-apoptotic and pro-apoptotic proteins and sirtuins family in male recreational long-distance runners

BMC Physiology

Volumn 10, Issue 1, 2010, Pages

  Marfe, Gabriella ^a   Tafani, Marco ^b   Pucci, Bruna ^b   Di Stefano, Carla ^a   Indelicato, Manuela ^b   Andreoli, Angela ^a   Russo, Matteo Antonio ^b,c   Sinibaldi Salimei, Paola ^a   Manzi, Vincenzo ^a  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

^b IRCCS SAN RAFFAELE PISANA   (Italy)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOSIS REGULATORY PROTEIN; BAX PROTEIN, HUMAN; CELL CYCLE PROTEIN; FORKHEAD TRANSCRIPTION FACTOR; FOXO3 PROTEIN, HUMAN; GADD45A PROTEIN, HUMAN; HEAT SHOCK PROTEIN 70; HEME OXYGENASE 1; HMOX1 PROTEIN, HUMAN; INDUCIBLE NITRIC OXIDE SYNTHASE; MESSENGER RNA; MITOCHONDRIAL PROTEIN; NOS2A PROTEIN, HUMAN; NUCLEAR PROTEIN; PROTEIN BAX; PROTEIN BCL 2; SIRT1 PROTEIN, HUMAN; SIRT3 PROTEIN, HUMAN; SIRT4 PROTEIN, HUMAN; SIRTUIN; SIRTUIN 1; SIRTUIN 3; SUPEROXIDE DISMUTASE;

ARTICLE; ENDURANCE; GENETICS; HUMAN; LIPID PEROXIDATION; LYMPHOCYTE; MALE; METABOLISM; OXIDATIVE STRESS; PHYSIOLOGY; RUNNING; SIGNAL TRANSDUCTION;

APOPTOSIS REGULATORY PROTEINS; BCL-2-ASSOCIATED X PROTEIN; CELL CYCLE PROTEINS; FORKHEAD TRANSCRIPTION FACTORS; HEME OXYGENASE-1; HSP70 HEAT-SHOCK PROTEINS; HUMANS; LIPID PEROXIDATION; LYMPHOCYTES; MALE; MITOCHONDRIAL PROTEINS; NITRIC OXIDE SYNTHASE TYPE II; NUCLEAR PROTEINS; OXIDATIVE STRESS; PHYSICAL ENDURANCE; PROTO-ONCOGENE PROTEINS C-BCL-2; RNA, MESSENGER; RUNNING; SIGNAL TRANSDUCTION; SIRTUIN 1; SIRTUIN 3; SIRTUINS; SUPEROXIDE DISMUTASE;

EID: 77951998780     PISSN: None     EISSN: 14726793     Source Type: Journal    
DOI: 10.1186/1472-6793-10-7     Document Type: Article

References (56)

1. Apoptosis-regulating proteins as targets for drug discovery. JC Reed, Trends Mol Med 2001 7 314 319 10.1016/S1471-4914(01)02026-3 11425640
2. Biochemical pathways of caspase activation during apoptosis. I Budihardjo H Oliver M Lutter X Luo X Wang, Annu Rev Cell Dev Biol 1999 15 269 290 10.1146/annurev.cellbio.15.1.269 10611963
3. Activated T cell death in vivo mediated by proapoptotic bcl-2 family member bim. DA Hildeman Y Zhu TC Mitchell P Bouillet A Strasser J Kappler P Marrack, Immunity 2002 16 759 767 10.1016/S1074-7613(02)00322-9 12121658
4. Free radicals, exercise, apoptosis, and heat shock proteins. E Fehrenbach H Northoff, Exerc Immunol Rev 2001 7 66 89 11579749
5. Lymphocyte apoptosis after exhaustive and moderate exercise. FC Mooren D Bloming A Lechtermann MM Lerch K Volker, J Appl Physiol 2002 93 147 153 12070198
6. Exercise-induced apoptosis of lymphocytes depends on training status. FC Mooren A Lechtermann K Volker, Med Sci Sports Exerc 2004 36 1476 1483 10.1249/01.MSS.0000139897.34521.E9 15354026
7. Apoptosis and exercise. S Phaneuf C Leeuwenburgh, Med Sci Sports Exerc 2001 33 393 396 10.1097/00005768-200103000-00010 11252065
8. High intensity exercise: A cause of lymphocyte apoptosis? M Mars S Govender A Weston V Naicker A Chuturgoon, Biochem Biophys Res Commun 1998 249 366 370 10.1006/bbrc.1998.9156 9712702
9. Lymphocyte apoptosis after exhaustive and moderate exercise. FC Mooren D Bloming A Lechtermann MM Lerch K Volker, J Appl Physiol 2002 93 147 153 12070198
10. Exercise-induced apoptosis of lymphocytes depends on training status. FC Mooren A Lechtermann K Volker, Med Sci Sports Exerc 2004 36 1476 1483 10.1249/01.MSS.0000139897.34521.E9 15354026
11. Prolonged exercise, lymphocyte apoptosis and F2-isoprostanes. A Steensberg J Morrow AD Toft H Bruunsgaard BK Pedersen, Eur J Appl Physiol 2002 87 38 42 10.1007/s00421-002-0584-6 12012074
12. Effects of exercise intensity on lymphocyte apoptosis induced by oxidative stress in men. JS Wang YH Huang, Eur J Appl Physiol 2005 95 290 297 10.1007/s00421-005-0005-8 16096840
13. Antioxidants and oxidative stress in exercise. LL Ji, Proc Soc Exp Biol Med 1999 222 283 292 10.1046/j.1525-1373.1999.d01-145.x 10601887
14. Superoxide radicals as precursors of mitochondrial hydrogen peroxide. G Loschen A Azzi C Richter L Flohe, FEBS Lett 1974 42 68 72 10.1016/0014-5793(74) 80281-4 4859511
15. Oxygen-derived free radicals in postischemic tissue injury. JM McCord, N Engl J Med 1985 312 159 163 2981404
16. NAD+/NADH and NADP+/NADPH in cellular functions and cell death: regulation and biological consequences. W Ying, Antioxid Redox Signal 2008 10 179 206 10.1089/ars.2007.1672 18020963
17. H2O2 accelerates cellular senescence by accumulation of acetylated p53 via decrease in the function of SIRT1 by NAD+ depletion. A Furukawa S Tada-Oikawa S Kawanishi S Oikawa, Cell Physiol Biochem 2007 20 45 54 17595514
18. The sir2 family of protein deacetylases. G Blander L Guarente, Annu Rev Biochem 2004 73 417 435 10.1146/annurev.biochem.73.011303.073651 15189148
19. Sirtuins: Sir2-related NAD-dependent protein deacetylases. BJ North E Verdin, Genome Biol 2004 5 224 10.1186/gb-2004-5-5-224 15128440
20. Negative control of p53 by Sir2alpha promotes cell survival under stress. J Luo AY Nikolaev S Imai D Chen F Su A Shiloh L Guarente W Gu, Cell 2001 107 137 148 10.1016/S0092-8674(01)00524-4 11672522
21. hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. H Vaziri SK Dessain E Ng Eaton SI Imai RA Frye TK Pandita L Guarente RA Weinberg, Cell 2001 107 149 159 10.1016/S0092-8674(01)00527-X 11672523
22. Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence. E Langley M Pearson M Faretta UM Bauer RA Frye S Minucci PG Pelicci T Kouzarides, EMBO J 2002 21 2383 2396 10.1093/emboj/21.10.2383 12006491
23. Modulation of NF-kappaB-dependent transcription and cell survival by the SIRT1 deacetylase. F Yeung JE Hoberg CS Ramsey MD Keller DR Jones RA Frye MW Mayo, EMBO J 2004 23 2369 2380 10.1038/sj.emboj.7600244 15152190
24. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. A Brunet LB Sweeney JF Sturgill KF Chua PL Greer Y Lin H Tran SE Ross R Mostoslavsky HY Cohen LS Hu HL Cheng MP Jedrychowski SP Gygi DA Sinclair FW Alt ME Greenberg, Science 2004 303 2011 2015 10.1126/science.1094637 14976264
25. Mammalian SIRT1 represses forkhead transcription factors. MC Motta N Divecha M Lemieux C Kamel D Chen W Gu Y Bultsma M McBurney L Guarente, Cell 2004 116 551 563 10.1016/S0092-8674(04)00126-6 14980222
26. Sirtuin gene expression in human mononuclear cells is modulated by caloric restriction. AB Crujeiras D Parra E Goyenechea JA Martnez, Eur J Clin Invest 2008 38 672 678 10.1111/j.1365-2362.2008.01998.x 18837744
27. The biochemistry of sirtuins. AA Sauve C Wolberger VL Schramm JD Boeke, Annu Rev Biochem 2006 75 435 465 10.1146/annurev.biochem.74.082803.133500 16756498
28. 8-Oxoguanosine and uracil repair of nuclear and mitochondrial DNA in red and white skeletal muscle of exercise trained old rats. Z Radak S Kumagai H Nakamoto S Goto, J Appl Physiol 2007 102 1696 1701 10.1152/japplphysiol.01051. 2006 17204574
29. Systemic adaptation to oxidative challenge induced by regular exercise. Z Radak HY Chung S Goto, Free Radic Biol Med 2008 44 153 159 10.1016/j. freeradbiomed.2007.01.029 18191751
30. Exercise training promotes SIRT1 activity in aged rats. N Ferrara B Rinaldi G Corbi V Conti P Stiuso S Boccuti G Rengo F Rossi A Filippelli, Rejuvenation Res 2008 11 139 150 10.1089/rej.2007.0576 18069916
31. Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor gamma coactivator-1alpha protein expressions in rat skeletal muscle. M Suwa H Nakano Z Radak S Kumagai, Metabolism 2008 57 986 998 10.1016/j.metabol.2008.02.017 18555842
32. SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase localized to mitochondria. P Onyango I Celic JM McCaffery JD Boeke AP Feinberg, Proc Natl Acad Sci USA 2002 99 13653 13658 10.1073/pnas.222538099 12374852
33. SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells. MC Haigis R Mostoslavsky KM Haigis K Fahie DC Christodoulou AJ Murphy DM Valenzuela GD Yancopoulos M Karow G Blander C Wolberger TA Prolla R Weindruch FW Alt L Guarente, Cell 2006 126 941 954 10.1016/j.cell.2006.06.057 16959573
34. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. H Ahn HS Kim S Song IH Lee J Liu A Vassilopoulos CX Deng T Finkel, Proc Natl Acad Sci USA 2008 105 14447 1445 10.1073/pnas.0803790105 18794531
35. SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial function and thermogenesis in brown adipocytes. T Shi F Wang E Stieren Q Tong, J Biol Chem 2005 280 13560 13567 10.1074/jbc.M414670200 15653680
36. Mechanisms and genes of cellular suicide. H Steller, Science 1995 267 1445 1449 10.1126/science.7878463 7878463
37. Apoptosis and aging: role of the mitochondria. M Pollack C Leeuwenburgh, J Gerontol A Biol Sci Med Sci 2001 56 475 B482 11682568
38. Antioxidant regulatory mechanisms in neutrophils and lymphocytes after intense exercise. MD Ferrer P Tauler A Sureda JA Tur A Pons, J Sports Sci 2009 27 49 58 10.1080/02640410802409683 19031335
39. Heat-shock protein 70 inhibits apoptosis by preventing recruitment of procaspase-9 to the Apaf-1 apoptosome. HM Beere BB Wolf K Cain DD Mosser A Mahboubi T Kuwana P Tailor RI Morimoto GM Cohen DR Green, Nat Cell Biol 2000 2 469 475 10.1038/35019501 10934466
40. Heat shock protein 70 inhibits apoptosis downstream of cytochrome c release and upstream of caspase-3 activation. CY Li JS Lee YG Ko JI Kim JS Seo, J Biol Chem 2000 275 25665 25671 10.1074/jbc.M906383199 10806214
41. Negative regulation of the Apaf-1 apoptosome by Hsp70. A Saleh SM Srinivasula L Balkir PD Robbins ES Alnemri, Nat Cell Biol 2000 2 476 483 10.1038/35019510 10934467
42. Exercise intensity and duration affect blood soluble HSP72. E Fehrenbach AM Niess K Voelker H Northoff FC Mooren, Int J Sports Med 2005 267 552 557 10.1055/s-2004-830334 (Pubitemid 41291177)
43. Changes of HSP72-expression in leukocytes are associated with adaptation to exercise under conditions of high environmental temperature. E Fehrenbach AM Niess R Veith HH Dickhuth H Northoff, J Leukoc Biol 2001 69 747 754 11358983
44. Forkhead transcription factor FOXO3a protects quiescent cells from oxidative stress. GJ Kops TB Dansen PE Polderman I Saarloos KW Wirtz PJ Coffer TT Huang JL Bos RH Medema BM Burgering, Nature 2002 419 316 321 10.1038/nature01036 12239572
45. DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein. H Tran A Brunet JM Grenier SR Datta AJ Fornace Jr PS Di Stefano LW Chiang ME Greenberg, Science 2002 296 530 534 10.1126/science.1068712 11964479
46. Substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5. C Schlicker M Gertz P Papatheodorou B Kachholz CF Becker C Steegborn, J Mol Biol 2008 382 790 801 10.1016/j.jmb.2008.07.048 18680753
47. Localization of mouse mitochondrial SIRT proteins: shift of SIRT3 to nucleus by co-expression with SIRT5. Y Nakamura M Ogura D Tanaka N Inagaki, Biochem Biophys Res. Commun 2008 366 174 179 10.1016/j.bbrc.2007.11.122 18054327
48. Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. WC Hallows S Lee JM Denu, Proc Natl Acad Sc USA 2006 103 10230 10235 10.1073/pnas.0604392103 (Pubitemid 44051149)
49. Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2. B Schwer J Bunkenborg RO Verdin JS Andersen E Verdin, Proc Natl Acad Sci USA 2006 103 10224 10229 10.1073/pnas.0603968103 16788062
50. Composition of the ESPEN Working Group. Composition of the ESPEN Working Group. Bioelectrical impedance analysis-part I: review of principles and methods. UG Kyle I Bosaeus AD De Lorenzo P Deurenberg M Elia JM Gámez BL Heitmann L Kent-Smith JC Melchior M Pirlich H Scharfetter AM Schols, Clin Nutr 2004 23 1226 1243 10.1016/j.clnu.2004.06.004 15380917
51. Bioelectrical impedance analysis-part II: utilization in clinical practice. UG Kyle I Bosaeus AD De Lorenzo P Deurenberg M Elia J Manuel Gámez B Lilienthal Heitmann L Kent-Smith JC Melchior M Pirlich H Scharfetter A M W J Schols C Pichard ESPEN, Clin Nutr 2004 23 1430 1453 10.1016/j.clnu.2004.09.012 15556267
52. Modelling the training response in athletes. EW Banister P Good G Holman CL Hamilton, Champaign, Illinois: Human kinetics 1986
53. A rapid and simple method for the isolation of apoptotic DNA fragments. M Herrmann HM Lorenz R Voll M Grünke W Woith JR Kalden, Nucleic Acids Res 1994 22 5506 5507 10.1093/nar/22.24.5506 7816645
54. Molecular Cloning:A Laboratory Manual. J Sambrook EF Fritsch T Maniatis, Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press 1989 2 2
55. Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues. S Streit CW Michalski M Erkan J Kleeff H Friess, Nat Protoc 2009 4 37 43 10.1038/nprot.2008.216 19131955
56. Statistical power analysis for the behavioral sciences. J Cohen, Hillsdale, NJ: Lawrence Erlbaum Associates 1998