Genetic engineering of algae for enhanced biofuel production

Eukaryotic Cell

Volumn 9, Issue 4, 2010, Pages 486-501

  Radakovits, Randor ^a   Jinkerson, Robert E ^a   Darzins, Al ^b   Posewitz, Matthew C ^a  

^a Department of Metallurgical and Materials Engineering   (United States)

^b NATIONAL RENEWABLE ENERGY LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOFUEL; FATTY ACID; HYDROGEN;

ALGA; CARBOHYDRATE METABOLISM; CYTOLOGY; GENETIC ENGINEERING; GENETICS; GENOME; GROWTH, DEVELOPMENT AND AGING; LIPID METABOLISM; METABOLISM; PHOTOSYNTHESIS; REVIEW; EUKARYOTE;

ALGAE; BIOFUELS; CARBOHYDRATE METABOLISM; FATTY ACIDS; GENETIC ENGINEERING; GENOME; HYDROGEN; LIPID METABOLISM; PHOTOSYNTHESIS; EUKARYOTA;

ALGAE; BACTERIA (MICROORGANISMS); CYANOBACTERIA; EMBRYOPHYTA; EUKARYOTA;

EID: 77950534012     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00364-09     Document Type: Review

References (218)

1. Aarts, M. G. M., C. J. Keijzer, W. J. Stiekema, and A. Pereira. 1995. Molecular characterization of the CER1 gene of Arabidopsis involved in epicuticular wax biosynthesis and pollen fertility. Plant Cell 7:2115-2127.
2. Apt, K. E., A. R. Grossman, and P. G. Kroth-Pancic. 1996. Stable nuclear transformation of the diatom Phaeodactylum tricornutum. Mol. Gen. Genet. 252:572-579.
3. Apt, K. E., L. Zaslavkaia, J. C. Lippmeier, M. Lang, O. Kilian, R. Wetherbee, A. R. Grossman, and P. G. Kroth. 2002. In vivo characterization of diatom multipartite plastid targeting signals. J. Cell Sci. 115:4061-4069.
4. Archibald, J. M., M. B. Rogers, M. Toop, K. Ishida, and P. J. Keeling. 2003. Lateral gene transfer and the evolution of plastid-targeted proteins in the secondary plastid-containing alga Bigelowiella natans. Proc. Natl. Acad. Sci. U. S. A. 100:7678-7683.
5. Armbrust, E. 1999. Identification of a new gene family expressed during the onset of sexual reproduction in the centric diatom Thalassiosira weissflogii. Appl. Environ. Microbiol. 65:3121-3128.
6. Armbrust, E. V., J. A. Berges, C. Bowler, B. R. Green, D. Martinez, N. H. Putnam, S. Zhou, A. E. Allen, K. E. Apt, M. Bechner, M. A. Brzezinski, B. K. Chaal, A. Chiovitti, A. K. Davis, M. S. Demarest, J. C. Detter, T. Glavina, D. Goodstein, M. Z. Hadi, U. Hellsten, M. Hildebrand, B. D. Jenkins, J. Jurka, V. V. Kapitonov, N. Kroger, W. W. Lau, T. W. Lane, F. W. Larimer, J. C. Lippmeier, S. Lucas, M. Medina, A. Montsant, M. Obornik, M. S. Parker, B. Palenik, G. J. Pazour, P. M. Richardson, T. A. Rynearson, M. A. Saito, D. C. Schwartz, K. Thamatrakoln, K. Valentin, A. Vardi, F. P. Wilkerson, and D. S. Rokhsar. 2004. The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science 306:79-86.
7. Atsumi, S., A. F. Cann, M. R. Connor, C. R. Shen, K. M. Smith, M. P. Brynildsen, K. J. Y. Chou, T. Hanai, and J. C. Liao. 2008. Metabolic engineering of Escherichia coli for 1-butanol production. Metab. Eng. 10: 305-311.
8. Atsumi, S., T. Hanai, and J. C. Liao. 2008. Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature 451:86-89.
9. Bachvaroff, T. R., G. T. Concepcion, C. R. Rogers, E. M. Herman, and C. F. Delwiche. 2004. Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genome. Protist 155:65-78.
10. Ball, S. G., and P. Deschamps. 2009. Starch metabolism, p. 1-40. In E. H. Harris and D. B. Stern (ed.), The Chlamydomonas sourcebook, second edition: organellar and metabolic processes, vol. 2, Academic Press, Oxford, England.
11. Beckmann, J., F. Lehr, G. Finazzi, B. Hankamer, C. Posten, L. Wobbe, and O. Kruse. 2009. Improvement of light to biomass conversion by de-regulaion of light-harvesting protein translation in Chlamydomonas reinhardtii. J. Biotechnol. 142:70-77.
12. Berthold, P., R. Schmitt, and W. Mages. 2002. An engineered Streptomyces hygroscopicus aph 7 gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii. Protist 153:401-412.
13. Bock, A., P. W. King, M. Blokesch, and M. C. Posewitz. 2006. Maturation of hydrogenases. Adv. Microb. Physiol. 51:1-71.
14. Boehlein, S. K., J. R. Shaw, J. D. Stewart, and L. C. Hannah. 2009. Characterization of an autonomously activated plant ADP-glucose pyrophosphorylase. Plant Physiol. 149:318-326.
15. Bowler, C., A. E. Allen, J. H. Badger, J. Grimwood, K. Jabbari, A. Kuo, U. Maheswari, C. Martens, F. Maumus, R. P. Otillar, E. Rayko, A. Salamov, K. Vandepoele, B. Beszteri, A. Gruber, M. Heijde, M. Katinka, T. Mock, K. Valentin, F. Verret, J. A. Berges, C. Brownlee, J. P. Cadoret, A. Chiovitti, C. J. Choi, S. Coesel, A. De Martino, J. C. Detter, C. Durkin, A. Falciatore, J. Fournet, M. Haruta, M. J. Huysman, B. D. Jenkins, K. Jiroutova, R. E. Jorgensen, Y. Joubert, A. Kaplan, N. Kroger, P. G. Kroth, J. La Roche, E. Lindquist, M. Lommer, V. Martin-Jezequel, P. J. Lopez, S. Lucas, M. Mangogna, K. McGinnis, L. K. Medlin, A. Montsant, M. P. Oudot-Le Secq, C. Napoli, M. Obornik, M. S. Parker, J. L. Petit, B. M. Porcel, N. Poulsen, M. Robison, L. Rychlewski, T. A. Rynearson, J. Schmutz, H. Shapiro, M. Siaut, M. Stanley, M. R. Sussman, A. R. Taylor, A. Vardi, P. von Dassow, W. Vyverman, A. Willis, L. S. Wyrwicz, D. S. Rokhsar, J. Weissenbach, E. V. Armbrust, B. R. Green, Y. Van de Peer, and I. V. Grigoriev. 2008. The Phaeodactylum genome reveals the evolutionary history of diatom genomes. Nature 456:239-244.
16. Casas-Mollano, J. A., J. Rohr, E. J. Kim, E. Balassa, K. van Dijk, and H. Cerutti. 2008. Diversification of the core RNA interference machinery in Chlamydomonas reinhardtii and the role of DCL1 in transposon silencing. Genetics 179:69-81.
17. Caspar, T., S. C. Huber, and C. Somerville. 1985. Alterations in growth, photosynthesis, and respiration in a starchless mutant of Arabidopsis thaliana (L.) deficient in chloroplast phosphoglucomutase activity. Plant Physiol. 79:11-17.
18. Caspar, T., T.-P. Lin, G. Kakefuda, L. Benbow, J. Preiss, and C. Somerville. 1991. Mutants of Arabidopsis with altered regulation of starch degradation. Plant Physiol. 95:1181-1188.
19. Cerutti, H., A. M. Johnson, N. W. Gillham, and J. E. Boynton. 1997. A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics 145:97-110.
20. Cheesbrough, T. M., and P. E. Kolattukudy. 1984. Alkane biosynthesis by decarbonylation of aldehydes catalyzed by a particulate preparation from Pisum sativum. Proc. Natl. Acad. Sci. U. S. A. 81:6613-6617.
21. Chen, H. L., S. S. Li, R. Huang, and H. J. Tsai. 2008. Conditional production of a functional fish growth hormone in the transgenic line of Nannochloropsis oculata (Eustigmatophyceae). J. Phycol. 44:768-776.
22. Chen, Y., Y. Wang, Y. Sun, L. Zhang, and W. Li. 2001. Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells. Curr. Genet. 39:365-370.
23. Cheney, D., B. Metz, and J. Stiller. 2001. Agrobacterium-mediated genetic transformation in the macroscopic marine red alga Porphyra yezoensis. J. Phycol. 37 (Suppl.):11.
24. Chepurnov, V. A., D. G. Mann, K. Sabbe, and W. Vyverman. 2004. Experimental studies on sexual reproduction in diatoms. Int. Rev. Cytol. 237:91-154.
25. Chepurnov, V. A., D. G. Mann, P. von Dassow, P. Vanormelingen, J. Gillard, D. Inzé, K. Sabbe, and W. Vyverman. 2008. In search of new tractable diatoms for experimental biology. Bioessays 30:692-702.
26. Chow, K. C., and W. L. Tung. 1999. Electrotransformation of Chlorella vulgaris. Plant Cell Rep. 18:778-780.
27. Cohen, J., K. Kim, M. Posewitz, M. L. Ghirardi, K. Schulten, M. Seibert, and P. King. 2005. Molecular dynamics and experimental investigation of H(2) and O(2) diffusion in [Fe]-hydrogenase. Biochem. Soc. Trans. 33:80-82.
28. Connor, M. R., and J. C. Liao. 2009. Microbial production of advanced transportation fuels in non-natural hosts. Curr. Opin. Biotechnol. 20:307-315.
29. Crépineau, F., T. Roscoe, R. Kaas, B. Kloareg, and C. Boyen. 2000. Characterisation of complementary DNAs from the expressed sequence tag analysis of life cycle stages of Laminaria digitata (Phaeophyceae). Plant Mol. Biol. 43:503-513.
30. Dawson, H. N., R. Burlingame, and A. C. Cannons. 1997. Stable transformation of Chlorella: rescue of nitrate reductase-deficient mutants with the nitrate reductase gene. Curr. Microbiol. 35:356-362.
31. Dehesh, K., P. Edwards, J. Fillatti, M. Slabaugh, and J. Byrne. 1998. KAS IV: a 3-ketoacyl-ACP synthase from Cuphea sp. is a medium chain specific condensing enzyme. Plant J. 15:383-390.
32. Dehesh, K., A. Jones, D. S. Knutzon, and T. A. Voelker. 1996. Production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by overexpression of Ch FatB 2, a thioesterase cDNA from Cuphea hookeriana. Plant J. 9:167-172.
33. Dehesh, K., H. Tai, P. Edwards, J. Byrne, and J. G. Jaworski. 2001. Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis. Plant Physiol. 125:1103-1114.
34. Deng, M.-D., and J. R. Coleman. 1999. Ethanol synthesis by genetic engineering in cyanobacteria. Appl. Environ. Microbiol. 65:523-528.
35. Dennis, M., and P. E. Kolattukudy. 1992. A cobalt-porphyrin enzyme converts a fatty aldehyde to a hydrocarbon and CO. Proc. Natl. Acad. Sci. U. S. A. 89:5306-5310.
36. Derelle, E., C. Ferraz, S. Rombauts, P. Rouze, A. Z. Worden, S. Robbens, F. Partensky, S. Degroeve, S. Echeynie, R. Cooke, Y. Saeys, J. Wuyts, K. Jabbari, C. Bowler, O. Panaud, B. Piegu, S. G. Ball, J. P. Ral, F. Y. Bouget, G. Piganeau, B. De Baets, A. Picard, M. Delseny, J. Demaille, Y. Van de Peer, and H. Moreau. 2006. Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features. Proc. Natl. Acad. Sci. U. S. A. 103:11647-11652.
37. De Riso, V., R. Raniello, F. Maumus, A. Rogato, C. Bowler, and A. Falciatore. 2009. Gene silencing in the marine diatom Phaeodactylum tricornutum. Nucleic Acids Res. 37:e96.
38. Deschamps, P., I. Haferkamp, C. d'Hulst, H. E. Neuhaus, and S. G. Ball. 2008. The relocation of starch metabolism to chloroplasts: when, why and how. Trends Plant Sci. 13:574-582.
39. Deschamps, P., I. Haferkamp, D. Dauvillee, S. Haebel, M. Steup, A. Buleon, J.-L. Putaux, C. Colleoni, C. d'Hulst, C. Plancke, S. Gould, U. Maier, H. E. Neuhaus, and S. Ball. 2006. Nature of the periplastidial pathway of starch synthesis in the cryptophyte Guillardia theta. Eukaryot. Cell 5:954-963.
40. Dexter, J., and P. Fu. 2009. Metabolic engineering of cyanobacteria for ethanol production. Energy Environ. Sci. 2:857-864.
41. Dismukes, G. C., D. Carrieri, N. Bennette, G. M. Ananyev, and M. C. Posewitz. 2008. Aquatic phototrophs: efficient alternatives to land-based crops for biofuels. Curr. Opin. Biotechnol. 19:235-240.
42. Doetsch, N. A., M. R. Favreau, N. Kuscuoglu, M. D. Thompson, and R. B. Hallick. 2001. Chloroplast transformation in Euglena gracilis: splicing of a group III twintron transcribed from a transgenic psbK operon. Curr. Genet. 39:49-60.
43. Dubini, A., F. Mus, M. Seibert, A. R. Grossman, and M. C. Posewitz. 2009. Flexibility in anaerobic metabolism as revealed in a mutant of Chlamydomonas reinhardtii lacking hydrogenase activity. J. Biol. Chem. 284:7201-7213.
44. Dunahay, T. G. 1993. Transformation of Chlamydomonas reinhardtii with silicon carbide whiskers. Biotechniques 15:452-455, 457-458,460.
45. Dunahay, T. G., E. E. Jarvis, and P. G. Roessler. 1995. Genetic transformation of the diatoms Cyclotella cryptica and Navicula saprophila. J. Phycol. 31:1004-1011.
46. Duran, W. R., and H. G. Pontis. 1977. Sucrose metabolism in green algae I. The presence of sucrose synthetase and sucrose phosphate synthetase. Mol. Cell Biochem. 16:149-152.
47. Reference deleted.
48. Eichler-Stahlberg, A., W. Weisheit, O. Ruecker, and M. Heitzer. 2009. Strategies to facilitate transgene expression in Chlamydomonas reinhardtii. Planta 229:873-883.
49. El-Sheekh, M. M. 1999. Stable transformation of the intact cells of Chlorella kessleri with high velocity microprojectiles. Biol. Plant. 42:209-216.
50. Ender, F., K. Godl, S. Wenzl, and M. Sumper. 2002. Evidence for autocatalytic cross-linking of hydroxyproline-rich glycoproteins during extracellular matrix assembly in Volvox. Plant Cell 14:1147-1160.
51. Falciatore, A., R. Casotti, C. Leblanc, C. Abrescia, and C. Bowler. 1999. Transformation of nonselectable reporter genes in marine diatoms. Mar. Biotechnol. 1:239-251.
52. Fischer, H., I. Robl, M. Sumper, and N. Kröger. 1999. Targeting and covalent modification of cell wall and membrane proteins heterologously expressed in the diatom Cylindrotheca fusiformis (Bacillariophyceae). J. Phycol. 35:113-120.
53. Fortman, J. L., S. Chhabra, A. Mukhopadhyay, H. Chou, T. S. Lee, E. Steen, and J. D. Keasling. 2008. Biofuel alternatives to ethanol: pumping the microbial well. Trends Biotechnol. 26:375-381.
54. Franklin, S., B. Ngo, E. Efuet, and S. P. Mayfield. 2002. Development of a GFP reporter gene for Chlamydomonas. Plant J. 30:733-744.
55. Fuhrmann, M., A. Hausherr, L. Ferbitz, T. Schödl, M. Heitzer, and P. Hegemann. 2004. Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene. Plant Mol. Biol. 55:869-881.
56. Fuhrmann, M., W. Oertel, and P. Hegemann. 1999. A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii. Plant J. 19:353-362.
57. Fulda, M., J. Schnurr, A. Abbadi, and E. Heinz. 2004. Peroxisomal acyl-CoA synthetase activity is essential for seedling development in Arabidopsis thaliana. Plant Cell 16:394-405.
58. Gan, S. Y., S. Qin, R. Y. Othman, D. Yu, and S. M. Phang. 2003. Transient expression of lacZ in particle bombarded Gracilaria changii (Gracilariales, Rhodophyta). J. Appl. Phycol. 15:345-349.
59. Germain, V., E. L. Rylott, T. R. Larson, S. M. Sherson, N. Bechtold, J. P. Carde, J. H. Bryce, I. A. Graham, and S. M. Smith. 2001. Requirement for 3-ketoacyl-CoA thiolase-2 in peroxisome development, fatty acid betaoxidation and breakdown of triacylglycerol in lipid bodies of Arabidopsis seedlings. Plant J. 28:1-12.
60. Ghirardi, M. L., A. Dubini, J. Yu, and P.-C. Maness. 2009. Photobiological hydrogen-producing systems. Chem. Soc. Rev. 38:52-61.
61. Ghirardi, M. L., M. C. Posewitz, P. C. Maness, A. Dubini, J. Yu, and M. Seibert. 2007. Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms. Annu. Rev. Plant Biol. 58:71-91.
62. Gibbons, G. F., K. Islam, and R. J. Pease. 2000. Mobilisation of triacylglycerol stores. Biochim. Biophys. Acta 1483:37-57.
63. Giroux, M. J., J. Shaw, G. Barry, B. G. Cobb, T. Greene, T. Okita, and L. C. Hannah. 1996. A single mutation that increases maize seed weight. Proc. Natl. Acad. Sci. U. S. A. 93:5824-5829.
64. Grossman, A. R., M. Croft, V. N. Gladyshev, S. S. Merchant, M. C. Posewitz, S. Prochnik, and M. H. Spalding. 2007. Novel metabolism in Chlamydomonas through the lens of genomics. Curr. Opin. Plant Biol. 10:190-198.
65. Gruber, A., S. Vugrinec, F. Hempel, S. B. Gould, U. G. Maier, and P. G. Kroth. 2007. Protein targeting into complex diatom plastids: functional characterisation of a specific targeting motif. Plant Mol. Biol. 64:519-530.
66. Hackett, J. D., T. E. Scheetz, H. S. Yoon, M. B. Soares, M. F. Bonaldo, T. L. Casavant, and D. Bhattacharya. 2005. Insights into a dinoflagellate genome through expressed sequence tag analysis. BMC Genomics 6:80.
67. Hanai, T., S. Atsumi, and J. C. Liao. 2007. Engineered synthetic pathway for isopropanol production in Escherichia coli. Appl. Environ. Microbiol. 73:7814-7818.
68. Hankamer, B., F. Lehr, J. Rupprecht, J. H. Mussgnug, C. Posten, and O. Kruse. 2007. Photosynthetic biomass and H2 production by green algae: from bioengineering to bioreactor scale-up. Physiol. Plant. 131:10-21.
69. Hawkins, R. L., and M. Nakamura. 1999. Expression of human growth hormone by the eukaryotic alga, Chlorella. Curr. Microbiol. 38:335-341.
70. Hayashi, M., K. Nito, R. Takei-Hoshi, M. Yagi, M. Kondo, A. Suenaga, T. Yamaya, and M. Nishimura. 2002. Ped3p is a peroxisomal ATP-binding cassette transporter that might supply substrates for fatty acid beta-oxidation. Plant Cell Physiol. 43:1.
71. Hellebust, J. A. 1965. Excretion of some organic compounds by marine phytoplankton. Limnol. Oceanogr. 10:192-206.
72. Hemschemeier, A., A. Melis, and T. Happe. 2009. Analytical approaches to photobiological hydrogen production in unicellular green algae. Photosynth. Res. 102:523-540.
73. Henry, I. M., M. D. Wilkinson, J. M. Hernandez, Z. Schwarz-Sommer, E. Grotewold, and D. F. Mandoli. 2004. Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum. BMC Plant Biol. 4:3.
74. Hirokawa, Y., S. Fujiwara, M. Suzuki, T. Akiyama, M. Sakamoto, S. Kobayashi, and M. Tsuzuki. 2008. Structural and physiological studies on the storage -polyglucan of haptophyte Pleurochrysis haptonemofera. Planta 227:589-599.
75. Hu, Q., M. Sommerfeld, E. Jarvis, M. Ghirardi, M. Posewitz, M. Seibert, and A. Darzins. 2008. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J. 54:621-639.
76. Huang, X., J. C. Weber, T. K. Hinson, A. C. Mathieson, and S. C. Minocha. 1996. Transient expression of the GUS reporter gene in the protoplasts and partially digested cells of Ulva lactuca L.(Chlorophyta). Bot. Mar. 39:467-474.
77. Huesemann, M. H., T. S. Hausmann, R. Bartha, M. Aksoy, J. C. Weissman, and J. R. Benemann. 2009. Biomass productivities in wild type and pigment mutant of Cyclotella sp. (diatom). Appl. Biochem. Biotechnol. 157:507-526.
78. Inui, M., M. Suda, S. Kimura, K. Yasuda, H. Suzuki, H. Toda, S. Yamamoto, S. Okino, N. Suzuki, and H. Yukawa. 2008. Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Appl. Microbiol. Biotechnol. 77:1305-1316.
79. Jain, R. K., M. Coffey, K. Lai, A. Kumar, and S. L. MacKenzie. 2000. Enhancement of seed oil content by expression of glycerol-3-phosphate acyltransferase genes. Biochem. Soc. Trans. 28:959-960.
80. Jako, C., A. Kumar, Y. Wei, J. Zou, D. L. Barton, E. M. Giblin, P. S. Covello, and D. C. Taylor. 2001. Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol. 126:861-874.
81. Jakobiak, T., W. Mages, B. Scharf, P. Babinger, K. Stark, and R. Schmitt. 2004. The bacterial paromomycin resistance gene, aphH, as a dominant selectable marker in Volvox carteri. Protist 155:381-393.
82. Janvilisri, T., H. Venter, S. Shahi, G. Reuter, L. Balakrishnan, and H. W. van Veen. 2003. Sterol transport by the human breast cancer resistance protein (ABCG2) expressed in Lactococcus lactis. J. Biol. Chem. 278:20645-20651.
83. Jarvis, E. E., and L. M. Brown. 1991. Transient expression of firefly luciferase in protoplasts of the green alga Chlorella ellipsoidea. Curr. Genet. 19:317-321.
84. Jiang, P., and J. E. Cronan, Jr. 1994. Inhibition of fatty acid synthesis in Escherichia coli in the absence of phospholipid synthesis and release of inhibition by thioesterase action. J. Bacteriol. 176:2814-2821.
85. Jiang, P., S. Qin, and C. K. Tseng. 2003. Expression of the lacZ reporter gene in sporophytes of the seaweed Laminaria japonica (Phaeophyceae) by gametophyte-targeted transformation. Plant Cell Rep. 21:1211-1216.
86. Kalscheuer, R., T. Stolting, and A. Steinbuchel. 2006. Microdiesel: Escherichia coli engineered for fuel production. Microbiology 152:2529-2536.
87. Kindle, K. L. 1990. High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. U. S. A. 87:1228-1232.
88. Kindle, K. L., R. A. Schnell, E. Fernandez, and P. A. Lefebvre. 1989. Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. J. Cell Biol. 109:2589-2601.
89. Klaus, D., J. B. Ohlrogge, H. E. Neuhaus, and P. Dörmann. 2004. Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase. Planta 219:389-396.
90. Kosourov, S. N., and M. Seibert. 2009. Hydrogen photoproduction by nutrient-deprived Chlamydomonas reinhardtii cells immobilized within thin alginate films under aerobic and anaerobic conditions. Biotechnol. Bioeng. 102:50-58.
91. Kruse, O., J. Rupprecht, K. P. Bader, S. Thomas-Hall, P. M. Schenk, G. Finazzi, and B. Hankamer. 2005. Improved photobiological H2 production in engineered green algal cells. J. Biol. Chem. 280:34170-34177.
92. Kubler, J. E., S. C. Minocha, and A. C. Mathieson. 1994. Transient expression of the GUS reporter gene in protoplasts of Porphyra miniata (Rhodophyta). J. Mar. Biotechnol. 1:165-169.
93. Kumar, S. V., R. W. Misquitta, V. S. Reddy, B. J. Rao, and M. V. Rajam. 2004. Genetic transformation of the green alga-Chlamydomonas reinhardtii by Agrobacterium tumefaciens. Plant Sci. 166:731-738.
94. Kurtzman, A. M., and D. P. Cheney. 1991. Direct gene transfer and transient expression in a marine red alga using the biolistic method. J. Phycol. 27(Suppl.):42.
95. Lapidot, M., D. Raveh, A. Sivan, S. M. Arad, and M. Shapira. 2002. Stable chloroplast transformation of the unicellular red alga Porphyridium species. Plant Physiol. 129:7-12.
96. Lardizabal, K., R. Effertz, C. Levering, J. Mai, M. C. Pedroso, T. Jury, E. Aasen, K. Gruys, and K. Bennett. 2008. Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean. Plant Physiol. 148:89-96.
97. Lee, J. W., L. Mets, and E. Greenbaum. 2002. Improvement of photosynthetic CO2 fixation at high light intensity through reduction of chlorophyll antenna size. Appl. Biochem. Biotechnol. 98:37-48.
98. Lee, S. K., H. Chou, T. S. Ham, T. S. Lee, and J. D. Keasling. 2008. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels. Curr. Opin. Biotechnol. 19:556-563.
99. Lehner, R., and D. E. Vance. 1999. Cloning and expression of a cDNA encoding a hepatic microsomal lipase that mobilizes stored triacylglycerol. Biochem. J. 343:1-10.
100. Liolios, K., K. Mavromatis, N. Tavernarakis, and N. C. Kyrpides. 2008. The Genomes On Line Database (GOLD) in 2007: status of genomic and metagenomic projects and their associated metadata. Nucleic Acids Res. 36:D475-D479.
101. Lluisma, A. O., and M. A. Ragan. 1997. Expressed sequence tags (ESTs) from the marine red alga Gracilaria gracilis. J. Appl. Phycol. 9:287-293.
102. Lu, X., H. Vora, and C. Khosla. 2008. Overproduction of free fatty acids in E. coli: implications for biodiesel production. Metab. Eng. 10:333-339.
103. Lu, Y., J. M. Steichen, S. E. Weise, and T. D. Sharkey. 2006. Cellular and organ level localization of maltose in maltose-excess Arabidopsis mutants. Planta 224:935-943.
104. Lumbreras, V., D. R. Stevens, and S. Purton. 1998. Efficient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron. Plant J. 14:441-447.
105. Maheswari, U., A. Montsant, J. Goll, S. Krishnasamy, K. R. Rajyashri, V. M. Patell, and C. Bowler. 2005. The diatom EST database. Nucleic Acids Res. 33:D344.
106. Marín-Navarro, J., A. L. Manuell, J. Wu, and S. P. Mayfield. 2007. Chloroplast translation regulation. Photosynth. Res. 94:359-374.
107. Martin, V. J. J., D. J. Pitera, S. T. Withers, J. D. Newman, and J. D. Keasling. 2003. Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat. Biotechnol. 21:796-802.
108. Maruyama, M., I. Horáková, H. Honda, X. Xing, N. Shiragami, and H. Unno. 1994. Introduction of foreign DNA into Chlorella saccharophila by electroporation. Biotechnol. Tech. 8:821-826.
109. Matsuzaki, M., O. Misumi, T. Shin-i, S. Maruyama, M. Takahara, S.-Y. Miyagishima, T. Mori, K. Nishida, F. Yagisawa, K. Nishida, Y. Yoshida, Y. Nishimura, S. Nakao, T. Kobayashi, Y. Momoyama, T. Higashiyama, A. Minoda, M. Sano, H. Nomoto, K. Oishi, H. Hayashi, F. Ohta, S. Nishizaka, S. Haga, S. Miura, T. Morishita, Y. Kabeya, K. Terasawa, Y. Suzuki, Y. Ishii, S. Asakawa, H. Takano, N. Ohta, H. Kuroiwa, K. Tanaka, N. Shimizu, S. Sugano, N. Sato, H. Nozaki, N. Ogasawara, Y. Kohara, and T. Kuroiwa. 2004. Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature 428:653-657.
110. Matthew, T., W. Zhou, J. Rupprecht, L. Lim, S. R. Thomas-Hall, A. Doebbe, O. Kruse, B. Hankamer, U. C. Marx, S. M. Smith, and P. M. Schenk. 2009. The metabolome of Chlamydomonas reinhardtii following induction of anaerobic H2 production by sulfur depletion. J. Biol. Chem. 284:23415-23425.
111. McManaman, J. L., T. D. Russell, J. Schaack, D. J. Orlicky, and H. Robenek. 2007. Molecular determinants of milk lipid secretion. J. Mammary Gland Biol. Neoplasia 12:259-268.
112. Melis, A. 2007. Photosynthetic H2 metabolism in Chlamydomonas reinhardtii (unicellular green algae). Planta 226:1075-1086.
113. Melis, A. 2009. Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximize efficiency. Plant Sci. 177:272-280.
114. Melis, A., L. Zhang, M. Forestier, M. L. Ghirardi, and M. Seibert. 2000. Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol. 122:127-136.
115. Mentewab, A., and C. N. Stewart. 2005. Overexpression of an Arabidopsis thaliana ABC transporter confers kanamycin resistance to transgenic plants. Nat. Biotechnol. 23:1177-1180.
116. Merchant, S. S., S. E. Prochnik, O. Vallon, E. H. Harris, S. J. Karpowicz, G. B. Witman, A. Terry, A. Salamov, L. K. Fritz-Laylin, L. Marechal-Drouard, W. F. Marshall, L. H. Qu, D. R. Nelson, A. A. Sanderfoot, M. H. Spalding, V. V. Kapitonov, Q. Ren, P. Ferris, E. Lindquist, H. Shapiro, S. M. Lucas, J. Grimwood, J. Schmutz, P. Cardol, H. Cerutti, G. Chanfreau, C. L. Chen, V. Cognat, M. T. Croft, R. Dent, S. Dutcher, E. Fernandez, H. Fukuzawa, D. Gonzalez-Ballester, D. Gonzalez-Halphen, A. Hallmann, M. Hanikenne, M. Hippler, W. Inwood, K. Jabbari, M. Kalanon, R. Kuras, P. A. Lefebvre, S. D. Lemaire, A. V. Lobanov, M. Lohr, A. Manuell, I. Meier, L. Mets, M. Mittag, T. Mittelmeier, J. V. Moroney, J. Moseley, C. Napoli, A. M. Nedelcu, K. Niyogi, S. V. Novoselov, I. T. Paulsen, G. Pazour, S. Purton, J. P. Ral, D. M. Riano-Pachon, W. Riekhof, L. Rymarquis, M. Schroda, D. Stern, J. Umen, R. Willows, N. Wilson, S. L. Zimmer, J. Allmer, J. Balk, K. Bisova, C. J. Chen, M. Elias, K. Gendler, C. Hauser, M. R. Lamb, H. Ledford, J. C. Long, J. Minagawa, M. D. Page, J. Pan, W. Pootakham, S. Roje, A. Rose, E. Stahlberg, A. M. Terauchi, P. Yang, S. Ball, C. Bowler, C. L. Dieckmann, V. N. Gladyshev, P. Green, R. Jorgensen, S. Mayfield, B. Mueller-Roeber, S. Rajamani, R. T. Sayre, P. Brokstein, I. Dubchak, D. Goodstein, L. Hornick, Y. W. Huang, J. Jhaveri, Y. Luo, D. Martinez, W. C. Ngau, B. Otillar, A. Poliakov, A. Porter, L. Szajkowski, G. Werner, K. Zhou, I. V. Grigoriev, D. S. Rokhsar, and A. R. Grossman. 2007. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318:245-250.
117. Metzger, P., and C. Largeau. 2005. Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Appl. Microbiol. Biotechnol. 66:486-496.
118. Meuser, J. E., G. Ananyev, L. E. Wittig, S. Kosourov, M. L. Ghirardi, M. Seibert, G. C. Dismukes, and M. C. Posewitz. 2009. Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms. J. Biotechnol. 142:21-30.
119. Michael, R., and D. J. Miller. 1998. Genetic transformation of dinoflagellates (Amphidinium and Symbiodinium): expression of GUS in microalgae using heterologous promoter constructs. Plant J. 13:427-435.
120. Michinaka, Y., T. Shimauchi, T. Aki, T. Nakajima, S. Kawamoto, S. Shigeta, O. Suzuki, and K. Ono. 2003. Extracellular secretion of free fatty acids by disruption of a fatty acyl-CoA synthetase gene in Saccharomyces cerevisiae. J. Biosci. Bioeng. 95:435-440.
121. Minoda, A., R. Sakagami, F. Yagisawa, T. Kuroiwa, and K. Tanaka. 2004. Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D. Plant Cell Physiol. 45:667-671.
122. Moellering, E. R., and C. Benning. 13 November 2009. RNAi silencing of a major lipid droplet protein affects lipid droplet size in Chlamydomonas reinhardtii. Eukaryot. Cell doi:10.1128/EC.00203-09.
123. Molnar, A., A. Bassett, E. Thuenemann, F. Schwach, S. Karkare, S. Ossowski, D. Weigel, and D. Baulcombe. 2009. Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. Plant J. 58:165-174.
124. Mouille, G., M. L. Maddelein, N. Libessart, P. Talaga, A. Decq, B. Delrue, and S. Ball. 1996. Preamylopectin processing: a mandatory step for starch biosynthesis in plants. Plant Cell 8:1353-1366.
125. Mus, F., A. Dubini, M. Seibert, M. C. Posewitz, and A. R. Grossman. 2007. Anaerobic acclimation in Chlamydomonas reinhardtii: anoxic gene expression, hydrogenase induction, and metabolic pathways. J. Biol. Chem. 282: 25475-25486.
126. Mussgnug, J. H., S. Thomas-Hall, J. Rupprecht, A. Foo, V. Klassen, A. McDowall, P. M. Schenk, O. Kruse, and B. Hankamer. 2007. Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. Plant Biotechnol. J. 5:802-814.
127. Nakajima, Y., M. Tsuzuki, and R. Ueda. 2001. Improved productivity by reduction of the content of light-harvesting pigment in Chlamydomonas perigranulata. J. Appl. Phycol. 13:95-101.
128. Nakajima, Y., and R. Ueda. 1997. Improvement of photosynthesis in dense microalgal suspension by reduction of light harvesting pigments. J. Appl. Phycol. 9:503-510.
129. Nguyen, M. T., S. P. Choi, J. Lee, J. H. Lee, and S. J. Sim. 2009. Hydrothermal acid pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. J. Microbiol. Biotechnol. 19:161-166.
130. Nikaido, I., E. Asamizu, M. Nakajima, Y. Nakamura, N. Saga, and S. Tabata. 2000. Generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis. DNA Res. 7:223-227.
131. Nishimoto, T., M. Nakano, S. Ikegami, H. Chaen, S. Fukuda, T. Sugimoto, M. Kurimoto, and Y. Tsujisaka. 1995. Existence of a novel enzyme converting maltose into trehalose. Biosci. Biotechnol. Biochem. 59:2189-2190.
132. Nojima, Y., A. Kibayashi, H. Matsuzaki, T. Hatano, and S. Fukui. 1999. Isolation and characterization of triacylglycerol-secreting mutant strain from yeast, Saccharomyces cerevisiae. J. Gen. Appl. Microbiol. 45:1-6.
133. O'Brien, E. A., L. B. Koski, Y. Zhang, L. S. Yang, E. Wang, M. W. Gray, G. Burger, and B. F. Lang. 2007. TBestDB: a taxonomically broad database of expressed sequence tags (ESTs). Nucleic Acids Res. 35:D445.
134. Ohlrogge, J. B., and J. G. Jaworski. 1997. Regulation of fatty acid synthesis. Annu. Rev. Plant Biol. 48:109-136.
135. Palenik, B., J. Grimwood, A. Aerts, P. Rouze, A. Salamov, N. Putnam, C. Dupont, R. Jorgensen, E. Derelle, S. Rombauts, K. Zhou, R. Otillar, S. S. Merchant, S. Podell, T. Gaasterland, C. Napoli, K. Gendler, A. Manuell, V. Tai, O. Vallon, G. Piganeau, S. Jancek, M. Heijde, K. Jabbari, C. Bowler, M. Lohr, S. Robbens, G. Werner, I. Dubchak, G. J. Pazour, Q. Ren, I. Paulsen, C. Delwiche, J. Schmutz, D. Rokhsar, Y. Van de Peer, H. Moreau, and I. V. Grigoriev. 2007. The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation. Proc. Natl. Acad. Sci. U. S. A. 104:7705-7710.
136. Panikashvili, D., S. Savaldi-Goldstein, T. Mandel, T. Yifhar, R. B. Franke, R. Hofer, L. Schreiber, J. Chory, and A. Aharoni. 2007. The Arabidopsis DESPERADO/AtWBC11 transporter is required for cutin and wax secretion. Plant Physiol. 145:1345-1360.
137. Park, M. O. 2005. New pathway for long-chain n-alkane synthesis via 1-alcohol in Vibrio furnissii M1. J. Bacteriol. 187:1426-1429.
138. Paulsen, B. S., T. Aslaksen, C. S. Freire-Nordi, and A. A. H. Vieira. 1998. Extracellular polysaccharides from Ankistrodesmus densus (Chlorophyceae). J. Phycol. 34:638-641.
139. Pienkos, P. T., and A. Darzins. 2009. The promise and challenges of microalgal-derived biofuels. Biofuels Bioprod. Bioref. 3:431-440.
140. Pighin, J. A., H. Zheng, L. J. Balakshin, I. P. Goodman, T. L. Western, R. Jetter, L. Kunst, and A. L. Samuels. 2004. Plant cuticular lipid export requires an ABC transporter. Science 306:702-704.
141. Polle, J. E. W., S. Kanakagiri, E. S. Jin, T. Masuda, and A. Melis. 2002. Truncated chlorophyll antenna size of the photosystems-a practical method to improve microalgal productivity and hydrogen production in mass culture. Int. J. Hydrogen Energy 27:1257-1264.
142. Polle, J. E. W., S. D. Kanakagiri, and A. Melis. 2003. tla1, a DNA insertional transformant of the green alga Chlamydomonas reinhardtii with a truncated light-harvesting chlorophyll antenna size. Planta 217:49-59.
143. Posewitz, M. C., A. Dubini, J. E. Meuser, M. Seibert, and M. L. Ghirardi. 2009. Hydrogenases, hydrogen production, and anoxia, p. 217-246. In E. H. Harris and D. B. Stern (ed.), The Chlamydomonas sourcebook, 2nd ed., vol. 2. Organellar and metabolic processes. Academic Press, Oxford, United Kingdom.
144. Posewitz, M. C., P. W. King, S. L. Smolinski, R. D. Smith, A. R. Ginley, M. L. Ghirardi, and M. Seibert. 2005. Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii. Biochem. Soc. Trans. 33:102-103.
145. Posewitz, M. C., P. W. King, S. L. Smolinski, L. Zhang, M. Seibert, and M. L. Ghirardi. 2004. Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase. J. Biol. Chem. 279:25711-25720.
146. Posewitz, M. C., S. L. Smolinski, S. Kanakagiri, A. Melis, M. Seibert, and M. L. Ghirardi. 2004. Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii. Plant Cell 16: 2151-2163.
147. Poulsen, N., P. M. Chesley, and N. Kröger. 2006. Molecular genetic manipulation of the diatom Thalassiosira pseudonana (Bacillariophyceae). J. Phycol. 42:1059-1065.
148. Poulsen, N., and N. Kroger. 2005. A new molecular tool for transgenic diatoms. FEBS J. 272:3413-3423.
149. Pruitt, K. D., T. Tatusova, and D. R. Maglott. 2005. NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins. Nucleic Acids Res. 33:D501-D504.
150. Qin, S., G. Q. Sun, P. Jiang, L. H. Zou, Y. Wu, and C. K. Tseng. 1999. Review of genetic engineering of Laminaria japonica (Laminariales, Phaeophyta) in China. Hydrobiologia 398:469-472.
151. Qin, S., D. Z. Yu, P. Jiang, C. Y. Teng, and C. K. Zeng. 2003. Stable expression of lacZ reporter gene in seaweed Undaria pinnatifida. High Technol. Lett. 13:87-89.
152. Quinn, J. M., and S. Merchant. 1995. Two copper-responsive elements associated with the Chlamydomonas Cyc6 gene function as targets for transcriptional activators. Plant Cell 7:623-638.
153. Ramachandra, T. V., D. M. Mahapatra, B. Karthick, and R. Gordon. 2009. Milking diatoms for sustainable energy: biochemical engineering versus gasoline-secreting diatom solar panels. Ind. Eng. Chem. Res. 48:8769-8788.
154. Ramazanov, A., and Z. Ramazanov. 2006. Isolation and characterization of a starchless mutant of Chlorella pyrenoidosa STL-PI with a high growth rate, and high protein and polyunsaturated fatty acid content. Phycol. Res. 54: 255-259.
155. Rashotte, A. M., M. A. Jenks, A. S. Ross, and K. A. Feldmann. 2004. Novel eceriferum mutants in Arabidopsis thaliana. Planta 219:5-13.
156. Ritte, G., M. Heydenreich, S. Mahlow, S. Haebel, O. Kötting, and M. Steup. 2006. Phosphorylation of C6-and C3-positions of glucosyl residues in starch is catalysed by distinct dikinases. FEBS Lett. 580:4872-4876.
157. Roesler, K., D. Shintani, L. Savage, S. Boddupalli, and J. Ohlrogge. 1997. Targeting of the Arabidopsis homomeric acetyl-coenzyme A carboxylase to plastids of rapeseeds. Plant Physiol. 113:75-81.
158. Roessler, P. G., Y. Chen, B. Liu, and C. N. Dodge. December 2009. Secretion of fatty acids by photosynthetic microorganisms. U.S. patent 20090298143.
159. Rylott, E. L., C. A. Rogers, A. D. Gilday, T. Edgell, T. R. Larson, and I. A. Graham. 2003. Arabidopsis mutants in short- and medium-chain acyl-CoA oxidase activities accumulate acyl-CoAs and reveal that fatty acid beta-oxidation is essential for embryo development. J. Biol. Chem. 278:21370-21377.
160. Sauer, N., and J. Stolz. 1994. SUC1 and SUC2: two sucrose transporters from Arabidopsis thaliana; expression and characterization in baker's yeast and identification of the histidine-tagged protein. Plant J. 6:67-77.
161. Scala, S., N. Carels, A. Falciatore, M. L. Chiusano, and C. Bowler. 2002. Genome properties of the diatom Phaeodactylum tricornutum. Plant Physiol. 129:993-1002.
162. Scharnewski, M., P. Pongdontri, G. Mora, M. Hoppert, and M. Fulda. 2008. Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling. FEBS J. 275:2765-2778.
163. Schiedlmeier, B., R. Schmitt, W. Müller, M. M. Kirk, H. Gruber, W. Mages, and D. L. Kirk. 1994. Nuclear transformation of Volvox carteri. Proc. Natl. Acad. Sci. U. S. A. 91:5080-5084.
164. Schneider-Belhaddad, F., and P. Kolattukudy. 2000. Solubilization, partial purification, and characterization of a fatty aldehyde decarbonylase from a higher plant, Pisum sativum. Arch. Biochem. Biophys. 377:341-349.
165. Sheehan, J., T. Dunahay, J. Benemann, and P. Roessler. 1998. A look back at the US Department of Energy's Aquatic Species Program-biodiesel from algae. Report no. NREL/TP-580-24190. National Renewable Energy Laboratory, Golden, Colorado
166. Shen, B., R. G. Jensen, and H. J. Bohnert. 1997. Increased resistance to oxidative stress in transgenic plants by targeting mannitol biosynthesis to chloroplasts. Plant Physiol. 113:1177-1183.
167. Sheveleva, E., W. Chmara, H. J. Bohnert, and R. G. Jensen. 1997. Increased salt and drought tolerance by D-ononitol production in transgenic Nicotiana tabacum L. Plant Physiol. 115:1211-1219.
168. Shigeoka, S., T. Ishikawa, M. Tamoi, Y. Miyagawa, T. Takeda, Y. Yabuta, and K. Yoshimura. 2002. Regulation and function of ascorbate peroxidase isoenzymes. J. Exp. Bot. 53:1305-1319.
169. Shimko, N., L. Liu, B. F. Lang, and G. Burger. 2001. GOBASE: the organelle genome database. Nucleic Acids Res. 29:128-132.
170. Shimogawara, K., S. Fujiwara, A. Grossman, and H. Usuda. 1998. High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics 148:1821-1828.
171. Shrager, J., C. Hauser, C. W. Chang, E. H. Harris, J. Davies, J. McDermott, R. Tamse, Z. Zhang, and A. R. Grossman. 2003. Chlamydomonas reinhardtii genome project. A guide to the generation and use of the cDNA information. Plant Physiol. 131:401-408.
172. Siloto, R. M. P., M. Truksa, D. Brownfield, A. G. Good, and R. J. Weselake. 2009. Directed evolution of acyl-CoA: diacylglycerol acyltransferase: development and characterization of Brassica napus DGAT1 mutagenized libraries. Plant Physiol. Biochem. 47:456-461.
173. Sizova, I., M. Fuhrmann, and P. Hegemann. 2001. A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii. Gene 277:221-229.
174. Smith, A. M. 2008. Prospects for increasing starch and sucrose yields for bioethanol production. Plant J. 54:546-558.
175. Smith, A. M., S. C. Zeeman, and S. M. Smith. 2005. Starch degradation. Annu. Rev. Plant Biol. 56:73-98.
176. Stark, D. M., K. P. Timmerman, G. F. Barry, J. Preiss, and G. M. Kishore. 1992. Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphorylase. Science 258:287-292.
177. Steinbrenner, J., and G. Sandmann. 2006. Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis. Appl. Environ. Microbiol. 72:7477-7484.
178. Stripp, S. T., G. Goldet, C. Brandmayr, O. Sanganas, K. A. Vincent, M. Haumann, F. A. Armstrong, and T. Happe. 2009. How oxygen attacks [FeFe] hydrogenases from photosynthetic organisms. Proc. Natl. Acad. Sci. U. S. A. 106:17331-17336.
179. Suda, Y., T. Yoshikawa, Y. Okuda, M. Tsunemoto, S. Tanaka, K. Ikeda, H. Miyasaka, M. Watanabe, K. Sasaki, and K. Harada. 2009. Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80. J. Biosci. Bioeng. 107:352-354.
180. Sun, Y., X. Gao, Q. Li, Q. Zhang, and Z. Xu. 2006. Functional complementation of a nitrate reductase defective mutant of a green alga Dunaliella viridis by introducing the nitrate reductase gene. Gene 377:140-149.
181. Sun, Y., Z. Yang, X. Gao, Q. Li, Q. Zhang, and Z. Xu. 2005. Expression of foreign genes in Dunaliella by electroporation. Mol. Biotechnol. 30:185-192.
182. Takeda, T., K. Miyao, M. Tamoi, H. Kanaboshi, H. Miyasaka, and S. Shigeoka. 2003. Molecular characterization of glutathione peroxidase-like protein in halotolerant Chlamydomonas sp. W80. Physiol. Plant. 117:467-475.
183. Tan, C., S. Qin, Q. Zhang, P. Jiang, and F. Zhao. 2005. Establishment of a micro-particle bombardment transformation system for Dunaliella salina. J. Microbiol. 43:361-365.
184. Tang, D. K., S. Y. Qiao, and M. Wu. 1995. Insertion mutagenesis of Chlamydomonas reinhardtii by electroporation and heterologous DNA. Biochem. Mol. Biol. Int. 36:1025-1035.
185. Tarczynski, M. C., R. G. Jensen, and H. J. Bohnert. 1993. Stress protection of transgenic tobacco by production of the osmolyte mannitol. Science 259:508-510.
186. Taylor, D. C., V. Katavic, J. Zou, S. L. MacKenzie, W. A. Keller, J. An, W. Friesen, D. L. Barton, K. K. Pedersen, and E. Michael Giblin. 2002. Field testing of transgenic rapeseed cv. Hero transformed with a yeast sn-2 acyltransferase results in increased oil content, erucic acid content and seed yield. Mol. Breed. 8:317-322.
187. Teng, C., S. Qin, J. Liu, D. Yu, C. Liang, and C. Tseng. 2002. Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis. J. Appl. Phycol. 14:497-500.
188. Tetali, S. D., M. Mitra, and A. Melis. 2007. Development of the lightharvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii is regulated by the novel Tla1 gene. Planta 225:813-829.
189. Tietge, U. J. F., A. Bakillah, C. Maugeais, K. Tsukamoto, M. Hussain, and D. J. Rader. 1999. Hepatic overexpression of microsomal triglyceride transfer protein (MTP) results in increased in vivo secretion of VLDL triglycerides and apolipoprotein B. J. Lipid Res. 40:2134-2139.
190. Tietge, U. J. F., C. Maugeais, W. Cain, D. Grass, J. M. Glick, F. C. de Beer, and D. J. Rader. 2000. Overexpression of secretory phospholipase A2 causes rapid catabolism and altered tissue uptake of high density lipoprotein cholesteryl ester and apolipoprotein A-I. J. Biol. Chem. 275:10077-10084.
191. Vigeolas, H., P. Waldeck, T. Zank, and P. Geigenberger. 2007. Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seedspecific promoter. Plant Biotechnol. J. 5:431-441.
192. Vioque, J., and P. E. Kolattukudy. 1997. Resolution and purification of an aldehyde-generating and an alcohol-generating fatty acyl-CoA reductase from pea leaves (Pisum sativum L.). Arch. Biochem. Biophys. 340:64-72.
193. Voelker, T. A., and H. M. Davies. 1994. Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase. J. Bacteriol. 176:7320-7327.
194. Voelker, T. A., A. C. Worrell, L. Anderson, J. Bleibaum, C. Fan, D. J. Hawkins, S. E. Radke, and H. M. Davies. 1992. Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257: 72-74.
195. Wackett, L. P., J. A. Frias, J. L. Seffernick, D. J. Sukovich, and S. M. Cameron. 2007. Genomic and biochemical studies demonstrating the absence of an alkane-producing phenotype in Vibrio furnissii M1. Appl. Environ. Microbiol. 73:7192-7198.
196. Wahlund, T. M., A. R. Hadaegh, R. Clark, B. Nguyen, M. Fanelli, and B. A. Read. 2004. Analysis of expressed sequence tags from calcifying cells of marine coccolithophorid (Emiliania huxleyi). Mar. Biotechnol. 6:278-290.
197. Wang, Z. T., N. Ullrich, S. Joo, S. Waffenschmidt, and U. Goodenough. 30 October 2009. Algal lipid bodies: stress induction, purification, and biochemical characterization in wild-type and starch-less Chlamydomonas reinhardtii. Eukaryot. Cell doi:10.1128/EC.00272-09.
198. Weber, A., C. Oesterhelt, W. Gross, A. Bräutigam, L. Imboden, I. Krassovskaya, N. Linka, J. Truchina, J. Schneidereit, and H. Voll. 2004. EST-analysis of the thermo-acidophilic red microalga Galdieriasulphuraria reveals potential for lipid A biosynthesis and unveils the pathway of carbon export from rhodoplasts. Plant Mol. Biol. 55:17-32.
199. Wingler, A., T. Fritzius, A. Wiemken, T. Boller, and R. A. Aeschbacher. 2000. Trehalose induces the ADP-gucose pyrophosphorylase gene, ApL3, and starch synthesis in Arabidopsis. Plant Physiol. 124:105-114.
200. Withers, S. T., S. S. Gottlieb, B. Lieu, J. D. Newman, and J. D. Keasling. 2007. Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity. Appl. Environ. Microbiol. 73:6277-6283.
201. Worden, A. Z., J. H. Lee, T. Mock, P. Rouze, M. P. Simmons, A. L. Aerts, A. E. Allen, M. L. Cuvelier, E. Derelle, M. V. Everett, E. Foulon, J. Grimwood, H. Gundlach, B. Henrissat, C. Napoli, S. M. McDonald, M. S. Parker, S. Rombauts, A. Salamov, P. Von Dassow, J. H. Badger, P. M. Coutinho, E. Demir, I. Dubchak, C. Gentemann, W. Eikrem, J. E. Gready, U. John, W. Lanier, E. A. Lindquist, S. Lucas, K. F. Mayer, H. Moreau, F. Not, R. Otillar, O. Panaud, J. Pangilinan, I. Paulsen, B. Piegu, A. Poliakov, S. Robbens, J. Schmutz, E. Toulza, T. Wyss, A. Zelensky, K. Zhou, E. V. Armbrust, D. Bhattacharya, U. W. Goodenough, Y. Van de Peer, and I. V. Grigoriev. 2009. Green evolution and dynamic adaptations revealed by genomes of the marine picoeukaryotes Micromonas. Science 324:268-272.
202. Wu, L., and R. G. Birch. 2007. Doubled sugar content in sugarcane plants modified to produce a sucrose isomer. Plant Biotechnol. J. 5:109-117.
203. Wykoff, D. D., J. P. Davies, A. Melis, and A. R. Grossman. 1998. The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii. Plant Physiol. 117:129-139.
204. Yoshimura, K., K. Miyao, A. Gaber, T. Takeda, H. Kanaboshi, H. Miyasaka, and S. Shigeoka. 2004. Enhancement of stress tolerance in transgenic tobacco plants overexpressing Chlamydomonas glutathione peroxidase in chloroplasts or cytosol. Plant J. 37:21-33.
205. Yu, L., S. Gupta, F. Xu, A. D. B. Liverman, A. Moschetta, D. J. Mangelsdorf, J. J. Repa, H. H. Hobbs, and J. C. Cohen. 2005. Expression of ABCG5 and ABCG8 is required for regulation of biliary cholesterol secretion. J. Biol. Chem. 280:8742-8747.
206. Yu, T.-S., H. Kofler, R. E. Hausler, D. Hille, U.-I. Flugge, S. C. Zeeman, A. M. Smith, J. Kossmann, J. Lloyd, G. Ritte, M. Steup, W.-L. Lue, J. Chen, and A. Weber. 2001. The Arabidopsis sex1 mutant is defective in the R1 protein, a general regulator of starch degradation in plants, and not in the chloroplast hexose transporter. Plant Cell 13:1907-1918.
207. Yu, T.-S., S. C. Zeeman, D. Thorneycroft, D. C. Fulton, H. Dunstan, W.-L. Lue, B. R. Hegemann, S.-Y. Tung, T. Umemoto, A. Chapple, D.-L. Tsai, S.-M. Wang, A. M. Smith, J. Chen, and S. M. Smith. 2005. -Amylase is not required for breakdown of transitory starch in Arabidopsis leaves. J. Biol. Chem. 280:9773-9779.
208. Yuan, L., T. A. Voelker, and D. J. Hawkins. 1995. Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. Proc. Natl. Acad. Sci. U. S. A. 92:10639-10643.
209. Zabawinski, C., N. Van Den Koornhuyse, C. D'Hulst, R. Schlichting, C. Giersch, B. Delrue, J.-M. Lacroix, J. Preiss, and S. Ball. 2001. Starchless mutants of Chlamydomonas reinhardtii lack the small subunit of a heterotetrameric ADP-glucose pyrophosphorylase. J. Bacteriol. 183:1069-1077.
210. Zaslavskaia, L. A., J. C. Lippmeier, P. G. Kroth, A. R. Grossman, and K. E. Apt. 2000. Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes. J. Phycol. 36:379-386.
211. Zaslavskaia, L. A., J. C. Lippmeier, C. Shih, D. Ehrhardt, A. R. Grossman, and K. E. Apt. 2001. Trophic conversion of an obligate photoautotrophic organism through metabolic engineering. Science 292:2073-2075.
212. Zeeman, S. C., T. Delatte, G. Messerli, M. Umhang, M. Stettler, T. Mettler, S. Streb, H. Reinhold, and O. Kotting. 2007. Starch breakdown: recent discoveries suggest distinct pathways and novel mechanisms. Funct. Plant Biol. 34:465-473.
213. Zeeman, S. C., F. Northrop, A. M. Smith, and T. A. Rees. 1998. A starch-accumulating mutant of Arabidopsis thaliana deficient in a chloroplastic starch-hydrolysing enzyme. Plant J. 15:357-365.
214. Zhao, T., W. Wang, X. Bai, and Y. Qi. 2009. Gene silencing by artificial microRNAs in Chlamydomonas. Plant J. 58:157-164.
215. Zheng, P., W. B. Allen, K. Roesler, M. E. Williams, S. Zhang, J. Li, K. Glassman, J. Ranch, D. Nubel, and W. Solawetz. 2008. A phenylalanine in DGAT is a key determinant of oil content and composition in maize. Nat. Genet. 40:367-372.
216. Zolman, B. K., I. D. Silva, and B. Bartel. 2001. The Arabidopsis pxa1 mutant is defective in an ATP-binding cassette transporter-like protein required for peroxisomal fatty acid beta-oxidation. Plant Physiol. 127:1266-1278.
217. Zorin, B., Y. Lu, I. Sizova, and P. Hegemann. 2009. Nuclear gene targeting in Chlamydomonas as exemplified by disruption of the PHOT gene. Gene 432:91-96.
218. Zou, J., V. Katavic, E. M. Giblin, D. L. Barton, S. L. MacKenzie, W. A. Keller, X. Hu, and D. C. Taylor. 1997. Modification of seed oil content and acyl composition in the Brassicaceae by expression of a yeast sn-2 acyltransferase gene. Plant Cell 9:909-923.