Ethanol synthesis by genetic engineering in cyanobacteria

Applied and Environmental Microbiology

Volumn 65, Issue 2, 1999, Pages 523-528

  Deng, Ming De ^a   Coleman, John R ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; ALCOHOL DEHYDROGENASE; CARBON; CARBON DIOXIDE; GLYCOGEN; INORGANIC COMPOUND; PYRUVATE DECARBOXYLASE; RIBULOSEBISPHOSPHATE CARBOXYLASE;

ARTICLE; CONTROLLED STUDY; CYANOBACTERIUM; ENZYME ACTIVITY; GENE; GENE EXPRESSION; GENETIC ENGINEERING; GRAM NEGATIVE BACTERIUM; IMMUNOBLOTTING; NONHUMAN; OPERON; PHOTOSYNTHESIS; SYNTHESIS; ZYMOMONAS MOBILIS;

BACTERIA (MICROORGANISMS); CYANOBACTERIA; NEGIBACTERIA; PROKARYOTA; SYNECHOCOCCUS; SYNECHOCOCCUS SP.; SYNECHOCOCCUS SP. PCC 6301; ZYMOMONAS MOBILIS;

EID: 0032976323     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.2.523-528.1999     Document Type: Article

References (39)

1. Aldrich, H. C., L. McDowell, M. de F. S. Barbosa, L. P. Yomano, R. K. Scopes, and L. O. Ingram. 1992. Immunocytochemical localization of glycolytic and fermentative enzymes in Zymomonas mobilis. J. Bacteriol. 174: 4504-4508.
2. Algar, E. M., and R. K. Scopes. 1985. Studies on cell-free metabolism: ethanol production by extracts of Zymomonas mobilis. J. Biotechnol. 2:275-287.
3. Allen, M. M. 1968. Simple conditions for growth of unicellular blue-green algae on plates. J. Phycol. 4:1-4.
4. Belay, A., Y. Ota, K. Miyakawa, and H. Shimamatsu. 1993. Current knowledge on potential health benefits of Spirulina. J. Appl. Phycol. 5:235-241.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
6. Brau, B., and H. Sahm. 1986. Cloning and expression of the structural gene for pyruvate decarboxylase of Zymomonas. Arch. Microbiol. 144:296-301.
7. Broedel, S. E., and R. E. Wolf, Jr. 1991. Growth-phase-dependent induction of 6-phosphogluconate dehydrogenase and glucose 6-phosphate dehydrogenase in the cyanobacterium Synechococcus sp. PCC7942. Gene 109:71-79.
8. Conway, T., Y. A. Osman, J. I. Konnan, E. M. Hoffmann, and L. O. Ingram. 1987. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169:949-954.
9. Conway, T., G. W. Sewell, Y. A. Osman, and L. O. Ingram. 1987. Cloning and sequencing the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169:2591-2597.
10. Geerts, D., A. Bovy, G. de Vrieze, M. Borrias, and P. Weisbeek. 1995. Inducible expression of heterologous genes targeted to a chromosomal platform in the cyanobacterium Synechococcus sp. PCC 7942. Microbiology 141:831-841.
11. Gendel, S., N. Straus, D. Pulleyblank, and J. Williams. 1983. Shuttle cloning vectors for the cyanobacterium Anacystis nidulans. J. Bacteriol. 156:148-154.
12. Gfeller, R. P., and M. Gibbs. 1984. Fermentation metabolism of Chlamydomonas reinhardtii: analysis of fermentation products from starch in the dark and light. Plant Physiol. 75:212-218.
13. Golden, S. S., M. S. Nalty, and D. C. Cho. 1989. Two functional psbD genes in the cyanobacterium Synechococcus sp. strain PCC 7942. J. Bacteriol. 171:4707-4713.
14. Golden, S. S., J. Brussian, and R. Haselkorn. 1987. Genetic engineering of the cyanobacterial chromosome. Methods Enzymol. 153:215-231.
15. Gruber, M. Y., B. R. Click, and J. E. Thompson. 1990. Cloned Mn-superoxide dismutase reduces oxidative stress in Escherichia coli and Anacystis nidulans. Proc. Natl. Acad. Sci. USA 87:2608-2612.
16. Heyer, H., and W. E. Krumbein. 1991. Excretion of fermentation products in the dark and anaerobically incubated cyanobacteria. Arch. Microbiol. 155: 284-287.
17. Hoppner, T. C., and H. W. Doelle. 1983. Purilication and kinetic characteristics of pyruvate decarboxylase and ethanol dehydrogenase from Zymomonas mobilis in relation to ethanol production. Eur. J. Appl. Microbiol. Biotechnol. 17:152-157.
18. Ingram, L. O., T. Conway, D. P. Clark, G. W. Sewell, and J. F. Preston. 1987. Genetic engineering of ethanol production in Escherichia coli. Appl. Environ. Microhiol. 53:2420-2425.
19. Karagouni, A. D., and J. H. Slater. 1979. Enzymes of the Calvin cycle and intermediary metabolism in the cyanobacterium Anancystis nidulans grown in chemostat culture. J. Gen. Microbiol. 115:369-376.
20. Laudenbach, D. E., C. G. Trick, and N. A. Straus. 1989. Cloning and characterization of an Anacystis nidulans R2 superoxide dismutase gene. Mol. Gen. Genet. 216:455-461.
21. Luinenburg, I., and J. R. Coleman. 1993. Expression of Escherichia coli phosphoenolpyruvate carboxylase in a cyanobacterium. Functional complementation of Synechococcus PCC 7942 ppc. Plant Physiol. 101:121-126.
22. Montenecourt, B. S. 1985. Zymomonas, a unique genus of bacteria, p. 261-289. In A. L. Demain and N. A. Solomon (ed.), Biology of industrial microorganisms. Benjamin-Cummings Publishing Co., Inc., Menlo Park, Calif.
23. Neale, A. D., R. K. Scopes, R. E. H. Wettenhall, and N. J. Hoogenraad. 1987. Nucleotide sequence of the pyruvate decarboxylase gene from Zymomonas mobilis. Nucleic Acids Res. 15:1753-1761.
24. Neale, A. D., R. K. Scopes, J. M. Kelly, and R. E. H. Wettenhall. 1986. The alcohol dehydrogenases of Zymomonas mobilis: purification by differential dye ligand chromatography, molecular characterization and physiological role. Eur. J. Biochem. 154:119-124.
25. Nomura, M., M. Ishitani, T. Takabe, A. K. Rai, and T. Takabe. 1995. Synechococcus sp. PCC7942 transformed with Escherichia coli bet genes produces glycine betaine from choline and acquires resistance to salt stress. Plant Physiol. 107:703-708.
26. 2 requiring phenotype. Plant Physiol. 91:505-513.
27. Radmer, R. J. 1996. Algal diversity and commercial algal products. BioScience 46:263-270.
28. Reith, M. E., D. E. Laudenbach, and N. A. Straus. 1986. Isolation and nucleotide sequence analysis of the ferredoxin I gene from the cyanobacterium Anacystis nidulans R2. J. Bacteriol. 168:1319-1324.
29. Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier. 1979. Generic assignments, strain histories and properties of pure cultures of cyanohacteria. J. Gen. Microbiol. 111:1-61.
30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. Shinozaki, K., and M. Sugiura. 1985. Genes for the large and small subunits of ribuloase-1,5-bisphosphate carboxylase/oxygenase constitute a single operon in the cyanobacterium Anacystis nidulans 6301. Mol. Gen. Genet. 200:27-32.
32. Smith, A. J. 1982. Modes of cyanobacterial carbon metabolism, p. 47-85. In N. G. Carr and B. A. Whitton (ed.), The biology of cyanobacteria. Blackwell Press, Oxford, United Kingdom.
33. Soltes-Rak, E., D. J. Kushner, D. D. Williams, and J. Coleman. 1993. Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942. Appl. Environ. Microbiol. 59:2404-2410.
34. Takeshima, Y., N. Takatsugu, M. Sugiura, and H. Hagiwara. 1994. High level expression of human superoxide dismutase in the cyanobacterium Anacystis nidulans 6301. Proc. Natl. Acad. Sci. USA 91:9685-9689.
35. Tandeau de Marsac, N., F. de la Torre, and J. Szulmajster. 1987. Expression of the larvicidal gene of Bacillus sphaericus 1853M in the cyanobacterium Anacystis nidulans R2. Mol. Gen. Genet. 209:396-398.
36. Thiel, T. 1995. Genetic analysis of cyanobacteria, p. 581-611. In D. A. Bryant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Press, Dordrecht, The Netherlands.
37. Van der Oust, J., B. A. Bulthuis, S. Feitz, K. Krab, and R. Kraayenhof. 1989. Fermentation metabolism of the unicellular cyanobacterium Cyanaothece PCC 7822. Arch. Microbiol. 152:415-419.
38. 2 to ethanol and other products. Am. Chem. Soc. Div. Fuel Chem. 41:1403-1406.
39. Zhang, M., C. Eddy, K. Deanda, M. Finkelstein, and S. Picataggio. 1995. Metabolic engineering of a penlose metabolism pathway in ethanologenic Zymomonas mobilis. Science 267:240-243.