A new molecular tool for transgenic diatoms: Control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette

FEBS Journal

Volumn 272, Issue 13, 2005, Pages 3413-3423

  Poulsen, Nicole ^a,b   Kröger, Nils ^a,b,c  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^c Georgia Institute of Technology   (United States)

Author keywords

Cylindrotheca fusiformis; Diatom transformation; Green fluorescent protein (GFP); Inducible gene expression; Nitrate reductase

Indexed keywords

COMPLEMENTARY DNA; GENOMIC DNA; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; NITRATE REDUCTASE; POLYPEPTIDE;

ARTICLE; BIOSYNTHESIS; CYLINDROTHECA FUSIFORMIS; DIATOM; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSLATION; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; DIATOMS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GREEN FLUORESCENT PROTEINS; MOLECULAR SEQUENCE DATA; NITRATE REDUCTASE; NITRATE REDUCTASES; NITRATES; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS (GENETICS); PROTEIN BIOSYNTHESIS; QUATERNARY AMMONIUM COMPOUNDS; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SEQUENCE HOMOLOGY, AMINO ACID; TERMINATOR REGIONS (GENETICS); TRANSCRIPTION, GENETIC; TRANSFORMATION, GENETIC;

BACILLARIOPHYTA; CYLINDROTHECA FUSIFORMIS; THALASSIOSIRA PSEUDONANA;

EID: 22144456432     PISSN: 1742464X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2005.04760.x     Document Type: Article

References (40)

1. Field C, Behrenfeld M, Randerson J & Falkowski P (1998) Primary production of the biosphere: integrating terrestrial and oceanic components. Science 281, 237-240.
2. Nelson D, Treguer P, Brzezinski M, Leynaert A & Queguiner B (1995) Production and dissolution of biogenic silica in the ocean: revised global estimates, comparisons with regional data and relationship to biogeneic sedimentation. Global Biogeochem Cycles 9, 359-372.
3. Sumper M & Kröger N (2004) Silica formation in diatoms: the function of long-chain polyamines and silaffins. J Mater Chem 14, 1-8.
4. Coradin T & Lopez P (2003) Biogenic silica patterning: simple chemistry or subtle biology? Chembiochem 4, 251-259.
5. Hildebrand M & Wetherbee R (2003) Components and control of silicification in diatoms. Prog Mol Subcell Biol 33, 11-57.
6. Armbrust EV, Berges JA, Bowler C, Green BR, Martinez D, Putnam NH, Zhou S, Allen AE, Apt KE, Bechner M, et al. (2004) The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science 306, 79-86.
7. Montsant A, Jabbari K, Maheswari U & Bowler C (2005) Comparative genomics of the pennate diatom Phaeodactylum tricornutum. Plant Physiol 137, 500-513.
8. Apt K, Kroth Pancic P & Grossman A (1996) Stable nuclear transformation of the diatom Phaeodactylum tricornutum. Mol Gen Genet 252, 572-579.
9. Fischer H, Robl I, Sumper M & Kröger N (1999) Targeting and covalent modification of cell wall and membrane proteins heterologously expressed in the diatom Cylindrotheca fusiformis (Bacillariophyceae). J Phycol 35, 113-120.
10. Dunahay T, Jarvis E & Roessler P (1995) Genetic transformation of the diatoms Cyclotella cryptica and Navicula saprophila. J Phycol 31, 1004-1012.
11. Kilian O & Kroth PG (2005) Identification and characterization of a new conserved motif within the presequence of proteins targeted into complex diatom plastids. Plant J 41, 175-183.
12. Lomas M (2004) Nitrate reductase and urease enzyme activity in the marine diatom Thalassiosira weissflogii (Bacillariophyceae): interactions among nitrogen substrates. Mar Biol 144, 37-44.
13. Gao Y, Smith GJ & Alberte RS (1993) Nitrate reductase from the marine diatom Skeletonema costatum (biochemical and immunological characterization). Plant Physiol 103, 1437-1445.
14. Quesada A & Fernandez E (1994) Expression of nitrate assimilation related genes in Chlamydomonas reinhardtii. Plant Mol Biol 24, 185-194.
15. Fernandez E, Schnell R, Ranum LP, Hussey SC, Silflow CD & Lefebvre PA (1989) Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 86, 6449-6453.
16. Cannons AC & Shiflett SD (2001) Transcriptional regulation of the nitrate reductase gene in Chlorella vulgaris: identification of regulatory elements controlling expression. Curr Genet 40, 128-135.
17. Song B & Ward B (2004) Molecular characterization of the assimilatory nitrate reductase gene and its expression in the marine green alga Dunaliella tertiolecta (Chlorophyceae). J Phycol 40, 721-731.
18. Allen AE, Ward BB & Song BK (2005) Characterization of diatom (Bacillariophyceae) nitrate reductase genes and their detection in marine phytoplankton communities. J Phycol 41, 95-104.
19. Zaslavskaia L, Lippmeier J, Shih C, Ehrhardt D, Grossman A & Apt K (2001) Trophic obligate conversion of an photoautotrophic organism through metabolic engineering. Science 292, 2073-2075.
20. Zhang G, Gurtu V & Kain SR (1996) An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem Biophys Res Commun 227, 707-711.
21. Reid BG & Flynn GC (1997) Chromophore formation in green fluorescent protein. Biochemistry 36, 6786-6791.
22. Kröger N (2001) The sweetness of diatom molecular engineering. J Phycol 37, 657-658.
23. Loppes R, Radoux M, Ohresser MC & Matagne RF (1999) Transcriptional regulation of the Niai gene encoding nitrate reductase in Chlamydomonas reinhardtii: effects of various environmental factors on the expression of a reporter gene under the control of the Nial promoter. Plant Mol Biol 41, 701-711.
24. Fernandez E & Cardenas J (1982) Regulation of the nitrate-reducing system enzymes in wild-type and mutant strains of Chlamydomonas reinhardtii. Mol Gen Genet 186, 164-169.
25. Fernandez E & Cardenas J (1989) Genetics and regulatory aspects of nitrate assimilation in algae. Molecular and Genetic Aspects of Nitrate Assimilation (Wray JL & Kinghorn JR, eds), pp. 101-124. Oxford Science Publications, Oxford.
26. Wilkie GS, Dickson KS & Gray NK (2003) Regulation of mRNA translation by 5′- and 3′-UTR-binding factors. Trends Biochem Sci 28, 182-188.
27. Campbell WH (1999) Nitrate reductase structure, function and regulation: bridging the gap between biochemistry and physiology. Annu Rev Plant Physiol Plant Mol Biol 50, 277-303.
28. Hildebrand M & Dahlin K (2000) Nitrate transporter genes from the diatom Cylindrotheca fusiformis (Bacillariophycea): mRNA levels controlled by nitrogen source and by the cell cycle. J Phycol 36, 702-713.
29. Hildebrand M (2005) Cloning and functional characterization of ammonium transporters from the marine diatom Cylindrotheca fusiformis (Bacillariophceae). J Phycol 41, 105-113.
30. Li J, Wang S, VanDusen WJ, Schultz LD, George HA, Herber WK, Chae HJ, Bentley WE & Rao G (2000) Green fluorescent protein in Saccharomyces cerevisiae: real-time studies of the GAL1 promoter. Biotechnol Bioeng 70, 187-196.
31. Yarger JG, Halverson HO & Hopper JE (1984) Regulation of galactokinase (GAL1) enzyme accumulation in Saccharomyces cerevisiae. Mol Cell Biochem 61, 173-182.
32. Martin-Jezequel V, Hildebrand M & Brzezinski MA (2000) Silicon metabolism in diatoms: implications for growth. J Phycol 36, 821-840.
33. Kröger N, Bergsdorf C & Sumper M (1996) Frustulins: domain conservation in a protein family associated with diatom cell walls. Eur J Biochem 239, 259-264.
34. Gatignol A, Durand H & Tiraby G (1988) Bleomycin resistance conferred by a drug-binding protein. FEBS Lett 230, 171-175.
35. Poulsen N & Kröger N (2004) Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana. J Biol Chem 279, 42993-42999.
36. Horton RM, Cai ZL, Ho SN & Pease LR (1990) Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques 8, 528-535.
37. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
38. Wilson CM (1983) Staining of proteins on gels: comparisons of dyes and procedures. Methods Enzymol 91, 236-247.
39. Kröger N & Wetherbee R (2000) Pleuralins are involved in theca differentiation in the diatom Cylindrotheca fusiformis. Protist 151, 263-273.
40. Thompson JD, Higgins DG & Gibson TJ (1994) Clustal-W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680.