Hepatic overexpression of microsomal triglyceride transfer protein (MTP) results in increased in vivo secretion of VLDL triglycerides and apolipoprotein B

Journal of Lipid Research

Volumn 40, Issue 11, 1999, Pages 2134-2139

  Tietge, Uwe J F ^a   Bakillah, Ahmed ^b   Maugeais, Cyrille ^a   Tsukamoto, Kazuhisa ^a   Hussain, Mahmood ^b   Rader, Daniel J ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

ApoB; Recombinant adenovirus expressing MTP; Triglyceride

Indexed keywords

APOLIPOPROTEIN B; APOLIPOPROTEIN B100; APOLIPOPROTEIN B48; LIPID TRANSFER PROTEIN; TRIACYLGLYCEROL; VERY LOW DENSITY LIPOPROTEIN;

ADENOVIRUS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL STRAIN COS1; CONTROLLED STUDY; FEMALE; GENE OVEREXPRESSION; LIPOPROTEIN SECRETION; LIVER MICROSOME; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; TRIACYLGLYCEROL BLOOD LEVEL; VIRUS RECOMBINANT;

ANIMALS; APOLIPOPROTEINS B; CARRIER PROTEINS; COS CELLS; FEMALE; GENE EXPRESSION; HUMANS; ISOTOPE LABELING; KINETICS; LIPOPROTEINS, VLDL; LIVER; MICE; MICE, INBRED C57BL; MICROSOMES, LIVER; SULFUR RADIOISOTOPES; TRANSFECTION; TRIGLYCERIDES;

EID: 0032717454     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Havel, R. J., and J. P. Kane. 1995. Introduction. Structure and metabolism of plasma lipoproteins. In The Metabolic and Molecular Basis of Inherited Disease. 7th edition. Vol. 2. C. R. Scriver, A. L. Beaudet, W. S. Sly, and D. Valle, editors. McGraw-Hill, New York. 1841-1851.
2. Young, S. 1990. Recent progress in understanding apolipoprotein B. Circulation. 82: 1574-1594.
3. Griffin, B. A., and A. Zampelas. 1995. Influence of dietary fatty acids on the atherogenic lipoprotein phenotype. Nutr. Res. Rev. 8: 1-26.
4. Wetterau, J. R., R. E. Gregg, T. W. Harrity, C. Arbeeny, M. Cap, F. Connolly, C. Chu, R. J. George, D. A. Gordon, H. Jamil, K. G. Jolibois, L. K. Kunselman, S. Lan, T.J. Maccagnan, B. Ricci, M. Yan, D. Young, Y. Chen, O. M. Fryszman, J. V. H. Logan, C. L. Musial, M. A. Poss, J. A. Robl, L. M. Simpkins, W. A. Slusarchyk, R. Sulsky, P. Taunk, D. R. Magnin, J. A. Tino, R. M. Lawrence, J. K. Dickson, and S. A. Biller. 1998. An MTP inhibitor that normalizes atherogenic lipoprotein levels in WHHL rabbits. Science. 282: 751-754.
5. Yao, Z., K. Tran, and R. S. McLeod. 1987. Intracellular degradation of newly synthesized apolipoprotein B. J. Lipid Res. 38: 1937-1953.
6. Dixon, J. L., S. Furukawa, and H. N. Ginsberg. 1991. Oleate stimulates secretion of apolipoprotein B-containing lipoproteins from Hep G2 cells by inhibiting early intracellular degradation of apolipoprotein B. J. Biol. Chem. 266: 5080-5086.
7. Pullinger, C. R., J. D. North, B. B. Teng, V. A. Rifici, A. E. Ronhild de Brito, and J. Scott. 1989. The apolipoprotein B gene is constitutively expressed in HepG2 cells: regulation of secretion by oleic acid, albumin, and insulin, and measurement of the mRNA half-life. J. Lipid Res. 30: 1065-1077.
8. Shelness, G. S., M. F. Ingram, X. F. Huang, and J. A. DeLozier. 1999. Apolipoprotein B in the rough endoplasmic reticulum: translation, translocation and the initiation of lipoprotein assembly. J. Nutr. 129: 456S-462S.
9. White, A. L., D. L. Graham, J. LeGros, R. J. Pease, and J. Scott. 1992. Oleate-mediated stimulation of apolipoprotein B secretion from rat hepatoma cells. A function of the ability of apolipoprotein B to direct lipoprotein assembly and escape presecretory degradation. J. Biol. Chem. 267: 15657-15664.
10. Sato, R., T. Imanaka, A. Takatsuki, and T. Takano. 1990. Degradation of newly synthesized apolipoprotein B-100 in a pre-Golgi compartment. J. Biol. Chem. 265: 11880-11884.
11. Rustaeus, S., K. Lindberg, P. Stillemark, C. Claesson, L. Asp, T. Larsson, J. Boren, and S. O. Olofsson. 1999. Assembly of very low density lipoprotein: a two-step process of apolipoprotein B core lipidation. J. Nutr. 129: 463S-466S.
12. Hussain, M. M., R. K. Kancha, Z. Zhou, J. Luchoomun, H. Zu, and A. Bakillah. 1996. Chylomicron assembly and catabolism: role of apolipoproteins and receptors. Biochim. Biophys. Acta. 1300: 151-170.
13. Ginsberg, H. N. 1997. Role of lipid synthesis, chaperone proteins and proteasomes in the assembly and secretion of apoprotein B-containing lipoproteins from cultured liver cells. Clin. Exp. Pharmacol. Physiol. 24: A29-A32.
14. White, D. A., A. J. Bennett, M. A. Billett, and A. M. Salter. 1998. The assembly of triacylglycerol-rich lipoproteins: an essential role for the microsomal triglyceride transfer protein. Br. J. Nutr. 80: 219-229.
15. Gordon, D. A. 1997. Recent advances in elucidating the role of the microsomal triglyceride transfer protein in apolipoprotein B lipoprotein assembly. Curr. Opin. Lipidol. 8: 131-137.
16. Wetterau, J. R., M. C. Lin, and H. Jamil. 1997. Microsomal triglyceride transfer protein. Biochim. Biopyhs. Acta. 1345: 136-150.
17. Sharp, D., L. Blinderman, K. A. Combs, B. Kienzle, B. Ricci, K. Wager-Smith, C. M. Gil, C. W. Turck, M. E. Bouna, D. J. Rader, L. P. Aggerbeck, R. E. Gregg, D. A. Gordon, and J. R. Wetterau. 1993. Cloning and gene defects in microsomal triglyceride transfer protein associated with abetalipoproteinemia. Nature. 365: 65-69.
18. Kane, J. P., and R. J. Havel. 1995. Disorders of the biogenesis and secretion of lipoproteins containing the B apolipoproteins. In The Metabolic and Molecular Basis of Inherited Disease. 7th edition. Vol. 2. C. R. Scriver, A. L. Beaudet, W. S. Sly, and D. Valle, editors. McGraw-Hill, New York. 1853-1885.
19. Gordon, D. A., H. Jamil, D. Sharp, D. Mullaney, Z. Yao, R. E. Gregg, and J. Wetterau. 1994. The secretion of apolipoprotein B-containing lipoproteins from HeLa-cells is dependent on expression of the microsomal triglyceride transfer protein and is regulated by lipid availability. Proc. Natl. Acad. Sci. USA. 91: 7628-7632.
20. Patel, S. B., and S. M. Grundy. 1996. Interactions between microsomal triglyceride transfer protein and apolipoprotein B within the endoplasmic reticulum in a heterologous expression system. J. Biol. Chem. 271: 18686-18694.
21. Leiper, J. M., J. D. Bayliss, R. J. Pease, D. J. Brett, J. Scott, and C. C. Shoulders. 1994. Microsomal triglyceride transfer protein, the abetalipoproteinemia gene product, mediates the secretion of apolipoprotein B-containing lipoproteins from heterologous cells. J. Biol. Chem. 269: 21951-21954.
22. Gretch, D. G., S. L. Sturley, L. Wang, B. A. Lipton, A. Dunning, K. A. Grunwald, J. R. Wetterau, Z. Yao, P. Talmud, and A. D. Attie. 1996. The amino-terminus of apolipoprotein B is necessary but not sufficient for microsomal triglyceride transfer protein responsiveness. J. Biol. Chem. 271: 8682-8691.
23. Jamil, H., D. A. Gordon, D. C. Eustice, C. M. Brooks, J. K. J. Dickson, Y. Chen, B. Ricci, C. H. Chu, T. W. Harrity, C. P. J. Ciosek, S. A. Biller, R. E. Gregg, and J. R. Wetterau. 1996. An inhibitor of the microsomal triglyceride transfer protein inhibits apoB secretion from HepG2 cells. Proc. Natl. Acad. Sci. USA. 93: 11991-11995.
24. Benoist, F., E. Nicodeme, and T. Grand-Perret. 1996. Microsomal triacylglycerol transfer protein prevents presecretory degradation of apolipoprotein B-100. A dithiothreitol-sensitive protease is involved. Eur. J. Biochem. 240: 713-720.
25. Haghpassand, M., D. Wilder, and J. B. Moberly. 1996. Inhibition of apolipoprotein B and triglyceride secretion in human hepatoma cells (HepG2). J. Lipid Res. 37: 1468-1480.
26. van Greevenbroek, M. M., M. G. Robertus-Teunissen, D. W. Erkelens, and T. W. de Bruin. 1998. Participation of the microsomal triglyceride transfer protein in lipoprotein assembly in CaCo-2 cells: interaction with saturated and unsaturated fatty acids. J. Lipid Res. 39: 173-185.
27. Wang, Y., R. S. McLeod, and Z. M. Yao. 1997. Normal activity of microsomal triglyceride protein is required for the oleate-induced secretion of very low density lipoproteins containing apoprotein B from McA-RH7777 cells. J. Biol. Chem. 272: 12272-12278.
28. Raabe, M., L. M. Flynn, C. H. Zlot, J. S. Wong, M. M. Veniant, R. L. Hamilton, and S. G. Young. 1998. Knockout of the abetalipoproteinemia gene in mice: reduced lipoprotein secretion in heterozygotes and embryonic lethality in homozygotes. Proc. Natl. Acad. Sci. USA. 95: 8686-8691.
29. Jamil, H., C. H. Chu, J. K. J. Dickson, Y. Chen, M. Yan, S. A. Biller, R. E. Gregg, J. R. Wetterau, and D. A. Gordon. 1998. Evidence that microsomal triglyceride transfer protein is limiting in the production of apolipoprotein B-containing lipoproteins in hepatic cells. J. Lipid Res. 39: 1448-1454.
30. Liao, W., K. Kobayashi, and L. Chan. 1999. Adenovirus-mediated overexpression of microsomal triglyceride transfer protein (MTP): mechanistic studies on the role of MTP in apolipoprotein B-100 biogenesis. Biochemistry. 38: 7532-7544.
31. Ye, X., M. Robinson, M. Batshaw, E. Furth, I. Smith, and J. M. Wilson. 1996. Prolonged metabolic correction in adult ornithine transcarbamylase-deficient mice with adenoviral vectors. J. Biol. Chem. 271:3639-3646.
32. Kozarsky, K. F., and J. M. Wilson. 1993. Gene therapy: adenovirus vectors. Curr. Opin. Genet. Dev. 3: 499-503.
33. Engelhardt, J. F., X. Ye, B. Doranz, and J. M. Wilson. 1994. Ablation of E2A in recombinant adenoviruses improves transgene persistence and decreases inflammatory response in mouse liver. Proc. Natl. Acad. Sci. USA. 91: 6196-6200.
34. Tsukamoto, K., P. Smith, J. M. Glick, and D. J. Rader. 1997. Liver-directed gene transfer and prolonged expression of three major human apoE isoforms in apoE-deficient mice. J. Clin. Invest. 100: 107-114.
35. Luchoomun, J., Z. Zhou, A. Bakillah, H. Jamil, and M. M. Hussain. 1997. Assembly and secretion of VLDL in nondifferentiated Caco-2 cells stably transfected with human recombinant apoB48 cDNA. Asterioscler. Thromb. Vasc. Biol. 17: 2955-2963.
36. Chang, B., W. Liao, L. Li, M. Nakamuta, D. Mack, and L. Chan. 1999. Liver-specific inactivation of the abetalipoproteinemia gene completely abrogates very low density lipoprotein/low density lipoprotein production in a viable conditional knockout mouse. J. Biol. Chem. 274: 6051-6055.
37. Borensztajn, J., M. S. Rone, and T. J. Kotlar. 1976. The inhibition in vivo of lipoprotein lipase (clearing-factor lipase) activity by Triton WR-1339. Biochem. J. 156: 539-543.
38. Ishikawa, T., and N. Fidge. 1979. Changes in the concentration of plasma lipoproteins and apoproteins following the administration of Triton WR 1339 to rats. J. Lipid Res. 20: 254-264.
39. Wu, X., A. Shang, H. Jiang, and H. N. Ginsberg. 1996. Low rates of apoB secretion from HepG2 cells result from reduced delivery of newly synthesized triglyceride to a "secretion-coupled" pool. J. Lipid Res. 37: 1198-1206.
40. Grundy, S. M., A. Chait, and J. D. Brunzell. 1987. Familial combined hyperlipidemia workshop. Arteriosclerosis. 7: 203-207.