Development of a liquid chromatographic system with fluorescent detection for β-secretase immobilized enzyme reactor on-line enzymatic studies

Journal of Pharmaceutical and Biomedical Analysis

Volumn 52, Issue 3, 2010, Pages 355-361

  Mancini, Francesca ^a   Andrisano, Vincenza ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Fluorescence detection; hrBACE1 IMER; Inhibitors screening; Methoxycoumaryl peptide substrate

Indexed keywords

AMYLOID PRECURSOR PROTEIN; BETA SECRETASE; GALLOCATECHIN; M 2420; OM 99 2; PANVERA PEPTIDE; PEPSTATIN; PEPTIDOMIMETIC AGENT; POLYPHENOL; STATIN PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME INHIBITION; ENZYME SPECIFICITY; ENZYME STABILITY; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; HYDROLYSIS; IC 50; IMMOBILIZED ENZYME REACTOR; ION TRAP MASS SPECTROMETRY; LIQUID CHROMATOGRAPHY; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL; PROCESS OPTIMIZATION;

AMYLOID PRECURSOR PROTEIN SECRETASES; ASPARTIC ACID ENDOPEPTIDASES; BIOREACTORS; CALIBRATION; CHROMATOGRAPHY, LIQUID; ENZYME STABILITY; ENZYMES, IMMOBILIZED; FEASIBILITY STUDIES; HUMANS; INHIBITORY CONCENTRATION 50; KINETICS; ONLINE SYSTEMS; RECOMBINANT PROTEINS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY, ULTRAVIOLET; SUBSTRATE SPECIFICITY;

EID: 77649180890     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jpba.2009.07.012     Document Type: Article

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