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Wilcox, C. S.; Glagovich, N. M. Program HOSTEST 5.6. Ph. D. Thesis, University of Pittsburgh, Pittsburgh, PA, 1994. Hostest program is designed to fit data to different binding models, which include both pure binding models, taking into consideration the formation of only one type of complex in solution (1:1, 1:2 or 2:1 receptor-substrate complex), and mixed binding models containing more than one type of complex in solution (for example, 1:1 and 1:2 or 1:1 and 2:1 receptor-substrate complex).
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Wilcox, C. S.; Glagovich, N. M. Program HOSTEST 5.6. Ph. D. Thesis, University of Pittsburgh, Pittsburgh, PA, 1994. Hostest program is designed to fit data to different binding models, which include both "pure" binding models, taking into consideration the formation of only one type of complex in solution (1:1, 1:2 or 2:1 receptor-substrate complex), and "mixed" binding models containing more than one type of complex in solution (for example, 1:1 and 1:2 or 1:1 and 2:1 receptor-substrate complex).
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58
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The binding studies were carried out in CDCl3 and DMSO-d6/CDCl3 mixtures at 25 d̀C. CDCl 3 was stored over activated molecular sieves and deacidified with Al2O3. The titration data were analyzed by non-linear regression analysis, using the program HOSTEST 5.6 see reference [53, For each system at least three titrations were carried out; for each titration 15-20 samples were prepared. Dilution experiments show that the receptors do not self-aggregate in the used concentration range. Error in Ka was <10, K11 corresponds to the 1:1 association constant. K 21 corresponds to the 2:1 receptor-sugar association constant. K 12 corresponds to the 1:2 receptor-sugar association constant. β21, K11 × K21, β12, K11 × K12. 55. Fielding, L. Tetrahedron
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Neidlein, U.; Diederich, F. Chem. Commun. 1996, 1493-1494. doi:10.1039/cc9960001493 For examples of receptors, which show strong di-vs. monosaccharide preference, see [14, 28, 30, 33, 37, 64].
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