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Volumn 131, Issue 48, 2009, Pages 17605-17614

FRET enabled real time detection of RNA-small molecule binding

Author keywords

[No Author keywords available]

Indexed keywords

ACCEPTOR EMISSION; AMINOGLYCOSIDES; ANTIBIOTIC BINDING; EMISSION QUENCHING; REAL-TIME DETECTION; ROBUST ANALYSIS; SMALL-MOLECULE BINDINGS; TITRATION CURVES;

EID: 72249111306     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja905767g     Document Type: Article
Times cited : (74)

References (73)
  • 21
    • 0142011647 scopus 로고    scopus 로고
    • Tor, Y. ChemBioChem 2003, 4, 998-1007.
    • (2003) ChemBioChem , vol.4 , pp. 998-1007
    • Tor, Y.1
  • 24
    • 33747894350 scopus 로고    scopus 로고
    • Hermann, T. Biochimie 2006, 88, 1021-1026.
    • (2006) Biochimie , vol.88 , pp. 1021-1026
    • Hermann, T.1
  • 25
    • 33747891811 scopus 로고    scopus 로고
    • Tor, Y. Biochimie 2006, 88, 1045-1051.
    • (2006) Biochimie , vol.88 , pp. 1045-1051
    • Tor, Y.1
  • 33
    • 0031051477 scopus 로고    scopus 로고
    • Aminoglycosides have been fluorescently labeled before, but typically by modifying the amines that are also essential for specific RNA binding. See
    • Aminoglycosides have been fluorescently labeled before, but typically by modifying the amines that are also essential for specific RNA binding. See: (a) Wang, Y.; Hamasaki, K.; Rando, R. R. Biochemistry 1997, 36, 768-779.
    • (1997) Biochemistry , vol.36 , pp. 768-779
    • Wang, Y.1    Hamasaki, K.2    Rando, R.R.3
  • 43
    • 72249088811 scopus 로고    scopus 로고
    • note
    • See Supporting Information for additional details.
  • 44
    • 72249115995 scopus 로고    scopus 로고
    • note
    • max 250-270 nm) and the intense blue emission of the acceptor.
  • 45
    • 72249091487 scopus 로고    scopus 로고
    • note
    • Based on crystal structures (PDB 2ET4 and 1LC4), the estimated distance between U1406 and the fluorophore on 11 or 12 would be less than 15 Å.
  • 46
    • 72249107294 scopus 로고    scopus 로고
    • note
    • The minimal A-site construct has been demonstrated to be an autonomous RNA domain capable of mimicking the function and antibiotics recognition features of the 16S rRNA. See ref 13 and 24.
  • 47
    • 72249120395 scopus 로고    scopus 로고
    • note
    • Modification of U1406 with isosteric nucleosides has been demonstrated to have a minimal detrimental effect on the A-site construct's folding and antibiotic recognition properties. See ref 36.
  • 48
    • 0031023096 scopus 로고    scopus 로고
    • Such a decrease in the quantum yield is common among fluorescent nucleosides upon incoporation into an RNA or DNA duplex. See ref 34-39 and
    • Such a decrease in the quantum yield is common among fluorescent nucleosides upon incoporation into an RNA or DNA duplex. See ref 34-39 and (a) Hawkins, M. E.; Pfleiderer, W.; Balis, F. M.; Porter, D.; Knutson, J. R. Anal. Biochem. 1997, 244, 86-95.
    • (1997) Anal. Biochem. , vol.244 , pp. 86-95
    • Hawkins, M.E.1    Pfleiderer, W.2    Balis, F.M.3    Porter, D.4    Knutson, J.R.5
  • 66
    • 33947241841 scopus 로고    scopus 로고
    • Tor, Y. Tetrahedron 2007, 63, 3425-3426.
    • (2007) Tetrahedron , vol.63 , pp. 3425-3426
    • Tor, Y.1
  • 71
    • 72249090220 scopus 로고    scopus 로고
    • note
    • According to crystal structures PDB 2ET4 and 1LC4, the positions of modification on the aminoglycosides are not involved in binding to the RNA.
  • 72
    • 0039302519 scopus 로고
    • Fluorescent brightening agents
    • Venkataraman, K., Ed.; Academic Press: New York
    • Gold, H. Fluorescent brightening agents. In The Chemistry of Synthetic Dyes; Venkataraman, K., Ed.; Academic Press: New York, 1971; Vol.V, pp 535-542.
    • (1971) The Chemistry of Synthetic Dyes , vol.5 , pp. 535-542
    • Gold, H.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.