메뉴 건너뛰기




Volumn 285, Issue 5434, 1999, Pages 1722-1725

Recognition of the codon-anticodon helix by ribosomal RNA

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

EID: 0033543589     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5434.1722     Document Type: Article
Times cited : (215)

References (45)
  • 2
    • 0027104840 scopus 로고
    • H. J. Grosjean, S. de Henau, D. M. Crothers, Proc. Natl. Acad. Sci. U.S.A. 75, 610 (1978); C. C. Kurland, Annu. Rev. Genet. 26, 29 (1992).
    • (1992) Annu. Rev. Genet. , vol.26 , pp. 29
    • Kurland, C.C.1
  • 4
    • 0030047916 scopus 로고    scopus 로고
    • M. V. Rodnina, T. Pape, R. Fricke, L. Kuhn, W. Wintermeyer, EMBO J. 14, 2613 (1995); J. Biol. Chem. 271, 646 (1996); T. Pape, W. Wintermeyer, M. Rodnina, EMBO J. 18, 3800 (1999).
    • (1996) J. Biol. Chem. , vol.271 , pp. 646
  • 5
    • 0033168212 scopus 로고    scopus 로고
    • M. V. Rodnina, T. Pape, R. Fricke, L. Kuhn, W. Wintermeyer, EMBO J. 14, 2613 (1995); J. Biol. Chem. 271, 646 (1996); T. Pape, W. Wintermeyer, M. Rodnina, EMBO J. 18, 3800 (1999).
    • (1999) EMBO J. , vol.18 , pp. 3800
    • Pape, T.1    Wintermeyer, W.2    Rodnina, M.3
  • 9
    • 0022916242 scopus 로고
    • D. Moazed and H. F. Noller, Cell 47, 985 (1986); J. Mol. Biol. 211, 135 (1990).
    • (1986) Cell , vol.47 , pp. 985
    • Moazed, D.1    Noller, H.F.2
  • 10
    • 0025070318 scopus 로고
    • D. Moazed and H. F. Noller, Cell 47, 985 (1986); J. Mol. Biol. 211, 135 (1990).
    • (1990) J. Mol. Biol. , vol.211 , pp. 135
  • 11
    • 0023238983 scopus 로고
    • D. Moazed and H. F. Noller, Nature 327, 389 (1987); R. Karimi and M. Ehrenberg, Eur. J. Biochem. 226, 355 (1994).
    • (1987) Nature , vol.327 , pp. 389
    • Moazed, D.1    Noller, H.F.2
  • 13
    • 0029825658 scopus 로고    scopus 로고
    • D. Fourmy, M. I. Recht, S. C. Blanchard, J. D. Puglisi, Science 274, 1367 (1996); D. Fourmy, S. Yoshizawa, J, D. Puglisi, J. Mol. Biol. 277, 333 (1998); S. Yoshizawa, D. Fourmy, J. D. Puglisi, EMBO J. 17, 6437 (1998); S. C. Blanchard, D. Fourmy, R. G. Eason, J. D. Puglisi, Biochemistry 37, 7716 (1998).
    • (1996) Science , vol.274 , pp. 1367
    • Fourmy, D.1    Recht, M.I.2    Blanchard, S.C.3    Puglisi, J.D.4
  • 14
    • 0032571359 scopus 로고    scopus 로고
    • D. Fourmy, M. I. Recht, S. C. Blanchard, J. D. Puglisi, Science 274, 1367 (1996); D. Fourmy, S. Yoshizawa, J, D. Puglisi, J. Mol. Biol. 277, 333 (1998); S. Yoshizawa, D. Fourmy, J. D. Puglisi, EMBO J. 17, 6437 (1998); S. C. Blanchard, D. Fourmy, R. G. Eason, J. D. Puglisi, Biochemistry 37, 7716 (1998).
    • (1998) J. Mol. Biol. , vol.277 , pp. 333
    • Fourmy, D.1    Yoshizawa, S.2    Puglisi, J.D.3
  • 15
    • 0032538956 scopus 로고    scopus 로고
    • D. Fourmy, M. I. Recht, S. C. Blanchard, J. D. Puglisi, Science 274, 1367 (1996); D. Fourmy, S. Yoshizawa, J, D. Puglisi, J. Mol. Biol. 277, 333 (1998); S. Yoshizawa, D. Fourmy, J. D. Puglisi, EMBO J. 17, 6437 (1998); S. C. Blanchard, D. Fourmy, R. G. Eason, J. D. Puglisi, Biochemistry 37, 7716 (1998).
    • (1998) EMBO J. , vol.17 , pp. 6437
    • Yoshizawa, S.1    Fourmy, D.2    Puglisi, J.D.3
  • 16
    • 0032568576 scopus 로고    scopus 로고
    • D. Fourmy, M. I. Recht, S. C. Blanchard, J. D. Puglisi, Science 274, 1367 (1996); D. Fourmy, S. Yoshizawa, J, D. Puglisi, J. Mol. Biol. 277, 333 (1998); S. Yoshizawa, D. Fourmy, J. D. Puglisi, EMBO J. 17, 6437 (1998); S. C. Blanchard, D. Fourmy, R. G. Eason, J. D. Puglisi, Biochemistry 37, 7716 (1998).
    • (1998) Biochemistry , vol.37 , pp. 7716
    • Blanchard, S.C.1    Fourmy, D.2    Eason, R.G.3    Puglisi, J.D.4
  • 18
    • 0344854791 scopus 로고    scopus 로고
    • note
    • Phe). The beads were pre-washed three times with buffer containing 0.3% bovine serum albumin (BSA) and once with buffer lacking BSA, and the unbound subunits were removed by washing three times with the 20 μl of binding buffer after the beads were trapped with a magnet.
  • 19
    • 0015527108 scopus 로고
    • S. Watanabe, J. Mol. Biol. 67, 443 (1972); R. Litt, J. M. Robertson, W. Wintermeyer, Biochemistry 25, 3245 (1986).
    • (1972) J. Mol. Biol. , vol.67 , pp. 443
    • Watanabe, S.1
  • 22
    • 0345717761 scopus 로고    scopus 로고
    • note
    • Phe in 20 μl of selection buffer. For selection in the presence of paromomycin, 100 μM drug was added. The active subunits were captured with beads and released by addition of 20 μl of 5 mM EDTA, 0.5% SDS, and 300 mM sodium acetate (pH 5.4) and incubation at room temperature for 20 min. Modifications were detected by primer extension with 12 independent primers to scan the entire sequence of 16S rRNA. Modification levels were quantified with a Phosphorimager (Molecular Dynamics), normalized to account for differences in lane loadings, and background levels estimated from unmodified sample lanes were subtracted. Modification of the same set of nucteotides as observed in (9) interfered with P-site tRNA binding, except that the interference at G1401 (N7) was not observed.
  • 24
    • 0345286087 scopus 로고    scopus 로고
    • note
    • L promoter and cl857, a thermolabile allele of the lambda repressor. Strain DH1 was used as the recipient of the final constructs.
  • 26
    • 0344423098 scopus 로고    scopus 로고
    • note
    • Phe in 10 μl of selection buffer. The ratio between mutant and wild-type ribosomal subunit population was analyzed by primer extension from allele-specific priming site V as described (16). The mutant population in each mixture was A1492G, 40 ±3%; A1492C, 40 ± 2%; A1493G, 32 ± 2%; A1493C, 23 ± 3%; A1492G-A1493G, 32 ± 3%: and A1492C-A1493C, 43 ± 2%.
  • 28
    • 0020352845 scopus 로고
    • R. A. Grajevskaja, Y. V. Ivanov, E. M. Saminsky, Eur. J. Biochem. 128, 47 (1982); S. V. Kirillow, E. M. Makarov, Y. Semenkov, FEBS Lett. 157, 91 (1983); R. Lill, J. M. Robertson, W. Wintermeyer, Biochemistry 23, 6710 (1984).
    • (1982) Eur. J. Biochem. , vol.128 , pp. 47
    • Grajevskaja, R.A.1    Ivanov, Y.V.2    Saminsky, E.M.3
  • 29
    • 0020534216 scopus 로고
    • R. A. Grajevskaja, Y. V. Ivanov, E. M. Saminsky, Eur. J. Biochem. 128, 47 (1982); S. V. Kirillow, E. M. Makarov, Y. Semenkov, FEBS Lett. 157, 91 (1983); R. Lill, J. M. Robertson, W. Wintermeyer, Biochemistry 23, 6710 (1984).
    • (1983) FEBS Lett. , vol.157 , pp. 91
    • Kirillow, S.V.1    Makarov, E.M.2    Semenkov, Y.3
  • 30
    • 0021766651 scopus 로고
    • R. A. Grajevskaja, Y. V. Ivanov, E. M. Saminsky, Eur. J. Biochem. 128, 47 (1982); S. V. Kirillow, E. M. Makarov, Y. Semenkov, FEBS Lett. 157, 91 (1983); R. Lill, J. M. Robertson, W. Wintermeyer, Biochemistry 23, 6710 (1984).
    • (1984) Biochemistry , vol.23 , pp. 6710
    • Lill, R.1    Robertson, J.M.2    Wintermeyer, W.3
  • 35
    • 0025002985 scopus 로고
    • K. Brady, A. Wei, D. Ringe, R. H. Abeles, Biochemistry 29, 7600 (1990); T. R. Burke et al., ibid. 35, 15989 (19%); L. H. Takahashi, R. Radhakrishnan, R. E. Rosenfield Jr., E. F. Meyer Jr., D. A. Trainor, J. Am Chem. Soc. 111, 3368 (1989).
    • (1990) Biochemistry , vol.29 , pp. 7600
    • Brady, K.1    Wei, A.2    Ringe, D.3    Abeles, R.H.4
  • 36
    • 0030467887 scopus 로고    scopus 로고
    • (19%)
    • K. Brady, A. Wei, D. Ringe, R. H. Abeles, Biochemistry 29, 7600 (1990); T. R. Burke et al., ibid. 35, 15989 (19%); L. H. Takahashi, R. Radhakrishnan, R. E. Rosenfield Jr., E. F. Meyer Jr., D. A. Trainor, J. Am Chem. Soc. 111, 3368 (1989).
    • Biochemistry , vol.35 , pp. 15989
    • Burke, T.R.1
  • 38
    • 0345286076 scopus 로고    scopus 로고
    • note
    • 639 → Phe mutant T7 RNA polymerase (29) with 2′ fluoro uridine triphosphate (UTP) (Amersham) or 2′ deoxy-UTP (Sigma) as substrates.
  • 42
    • 0032516432 scopus 로고    scopus 로고
    • A. A. Szewczak, L. Ortoteva-Donnelly, S. P. Ryder, E. Moncoeur, S. A. Strobel, Nature Struct. Biol. 5, 1037 (1998); G. J. Narlikar and D. Herschlag, Biochemistry 37, 9902 (1998).
    • (1998) Biochemistry , vol.37 , pp. 9902
    • Narlikar, G.J.1    Herschlag, D.2
  • 45
    • 0344423084 scopus 로고    scopus 로고
    • note
    • 639 → Phe mutant T7 RNA polymerase gene. Supported by grants from NIH (GMS1266), the Packard Foundation, and the Lucille P. Markey Charitable Trust. S.Y. was supported by a grant from the Japan Society for the Promotion of Science. D.F. was supported by a grant From Institut National de la Santé et de la Recherche Médicale.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.