Localization and quantitation of free sulfhydryl in recombinant monoclonal antibodies by differential labeling with 12C and 13C iodoacetic acid and LC-MS analysis

Analytical Chemistry

Volumn 81, Issue 19, 2009, Pages 8101-8108

  Xiang, Tao ^a   Chumsae, Chris ^a   Liu, Hongcheng ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION REAGENTS; COMMON FEATURES; CYSTEINE RESIDUES; CYSTEINE-CONTAINING PEPTIDES; DISULFIDE BONDS; DOMAIN STRUCTURE; IODOACETIC ACID; RECOMBINANT MONOCLONAL ANTIBODY; REVERSED-PHASE CHROMATOGRAPHY; SULFHYDRYL;

ALKYLATION; AMINES; CHROMATOGRAPHIC ANALYSIS; HYDROCARBONS; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PEPTIDES; SURFACE PLASMON RESONANCE;

ACIDS;

CYSTEINE; DISULFIDE; IODOACETIC ACID C 12; IODOACETIC ACID C 13; MONOCLONAL ANTIBODY; RADIOISOTOPE; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ALKYLATION; ANTIBODY STRUCTURE; ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; REVERSED PHASE LIQUID CHROMATOGRAPHY;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; CARBON ISOTOPES; CARBON RADIOISOTOPES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; IODOACETIC ACID; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SULFHYDRYL COMPOUNDS;

EID: 70349630449     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac901311y     Document Type: Article

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