Effect of copper sulfate on performance of a serum-free CHO cell culture process and the level of free thiol in the recombinant antibody expressed

Biotechnology Progress

Volumn 21, Issue 2, 2005, Pages 550-553

  Chaderjian, Wendy B ^a   Chin, Edward T ^a   Harris, Reed J ^a   Etcheverry, Tina M ^a  

^a GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; BIOTECHNOLOGY; CHEMICAL BONDS; COPPER COMPOUNDS; GROWTH KINETICS; SULFUR COMPOUNDS;

COPPER SULFATE; CYSTEINE; PRODUCT QUALITY; THIOLS;

CELL CULTURE;

ANTIBODY; COPPER SULFATE; RECOMBINANT PROTEIN; THIOL DERIVATIVE;

ANIMAL; ARTICLE; BIOREACTOR; CHEMISTRY; CHO CELL; CULTURE MEDIUM; HAMSTER;

ANIMALS; ANTIBODIES; BIOREACTORS; CHO CELLS; COPPER SULFATE; CRICETINAE; CULTURE MEDIA, SERUM-FREE; RECOMBINANT PROTEINS; SULFHYDRYL COMPOUNDS;

CRICETINAE; CRICETULUS GRISEUS;

EID: 16344374566     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp0497029     Document Type: Article

References (15)

1. Edelman, G. M. The covalent Structure of an Entire Gamma G Immunoglobulin Molecule. Proc. Natl. Acad. Sci. U.S.A. 1969, 63, 78-85.
2. Zhang, W. Free Sulfhydryl in Recombinant Monoclonal Antibodies. Biotechnol. Prog. 2002, 18, 509-513.
3. Beinert, H. Copper in Biological Systems. J. Inorg. Biochem. 1991, 44, 173-218.
4. Li, N. C.; Manning, R. A. Some Metal Complexes of Sulfur-Containing Amino Acids. J. Am. Chem. Soc. 1955, 77, 5225-5228.
5. Cooper, A. J. L. Biochemistry of Sulfur-Containing Amino Acids. Annu. Rev. Biochem. 1983, 52, 187-222.
6. Goldstein, S.; Czapski. G. Mechanisms of the Reaction of Some Copper Complexes in the Presence of DNA with Superoxide Anion, Hydrogen Peroxide, and Molecular Oxygen. Free Radical Biol. Med. 1986, 2, 3-11.
7. Hultberg, B.; Andersson, A.; Isaksson, A. Copper Ions Differ from Other Thiol Reactive Metal Ions in Their Effects on the Concentration and Redox Status of Thiols in HeLa Cell Cultures. Toxicology 1997, 117, 89-97.
8. Camakaris, J.; Petris, M. J.; Baily, L.; Shen, P.; Lockhart, P.; Glover, T. W.; Barcroft, C. L.; Patton, J.; Mercer, J. F. B. Gene Amplification of the Menkes (MNK; ATP7A) P-type ATPse of CHO Cells is Associated with Copper Resistance and Enhanced Copper Efflux. Human Mol. Genetics 1995, 4, 2117-2123.
9. Steinebach, O. M.; Wolterbeek, H. T. Effects of Copper on Rat Hepatoma HTC Cells and Primary Cultured Rat Hepatocytes. J. Inorg. Biochem. 1994, 53, 27-48.
10. Schmidt, D. Increasing the Growth-Promoting Ability of Cell Culture Serum by Adding Soluble Heavy Metal Compounds Preferably of Cu or Zn. German Patent 82-03602, 1982.
11. Sakai, Y.; Koibuchi, M.; Sakoda, A.; Suzuki, M. Growth Inhibition and Heavy Metal Accumulation in CHO Cells. Cytotechnology 1994, 14, Suppl.1, 7.36.
12. Smith, M. A.; Easton, M.; Everett, P.; Lewis, G.; Payne, M.; Reveros-Morena, V.; Allen, G. Specific Cleavage of Immunoglobulin G by Copper Ions. Int. J. Peptide Protein Res. 1996, 48, 48-55.
13. Urlaub, G.; Chasin, L. A. Isolation of Chinese Hamster Cell Mutants Deficient in Dihydrofolate Reductase Activity. Proc. Natl. Acad. Sci. U.S.A. 1980, 77, 4216-4220.
14. Kaufman R. J.; Sharpe, P. A. Amplification and Expression of Sequences Cotransfected with a Modular Dihydrofolate Reductase Complementary DNA Gene. J. Mol. Biol. 1982, 159, 601-621.
15. Fahrner, R. L.; Knudsen, H. L.; Basey, C. D.; Galan, W.; Feuerhelm, D.; Vanderlaan. M.; Blank, G. S. Industrial Purification of Pharmaceutical Antibodies: Development, Operation, and Validation of Chromatography Processes. Biotechnol. Genetic Eng. Rev. 2001, 18, 301-327.