Novel sequential ChIP and simplified basic ChIP protocols for promoter co-occupancy and target gene identification in human embryonic stem cells

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Medeiros, Ricardo B ^a   Papenfuss, Kate J ^a   Hoium, Brian ^a   Coley, Kristen ^a   Jadrich, Joy ^a   Goh, Saik Kia ^a   Elayaperumal, Anuratha ^a   Herrera, Julio E ^a   Resnik, Ernesto ^a   Ni, Hsiao Tzu ^a  

^a R and D Systems Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE BEADS; ANTIBODY-ANTIGEN; CHROMATIN IMMUNOPRECIPITATION; DENSITOMETRY; EMBRYONIC STEM CELLS; HIGH-THROUGHPUT; HIGH-THROUGHPUT APPROACHES; HUMAN EMBRYONIC STEM CELLS; IN-VIVO; KNOWN TARGET GENES; MOLECULAR MECHANISM; RAPID IDENTIFICATION; REAL-TIME PCR; STATISTICAL ANALYSIS; SYSTEMATIC IDENTIFICATION; TARGET GENES; TRANSCRIPTIONAL REGULATION; TRANSCRIPTIONAL REPRESSORS;

ANTIBODIES; ASSAYS; CELL CULTURE; CELL GROWTH; CELL MEMBRANES; CENTRIFUGATION; GENES; MAGNETIC SEPARATION; TARGETS;

BIOCHIPS;

AGAROSE; BMI1 PROTEIN; KRUPPEL LIKE FACTOR 4; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; OLIGODENDROCYTE TRANSCRIPTION FACTOR 2; SMAD2 PROTEIN; SMAD3 PROTEIN; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR RUNX2; TRANSCRIPTION FACTOR SOX17; TRANSCRIPTION FACTOR SOX2;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CENTRIFUGATION; CHROMATIN IMMUNOPRECIPITATION; COMPUTER PROGRAM; CONTROLLED STUDY; DENSITOMETRY; EMBRYONIC STEM CELL; GENE EXPRESSION; GENE IDENTIFICATION; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IN VIVO STUDY; MAGNETIC SEPARATION; MOUSE; NONHUMAN; PROMOTER REGION; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING; ANIMAL; CELL LINE; COMPARATIVE STUDY; CYTOLOGY; METABOLISM; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; CELL LINE; CHROMATIN IMMUNOPRECIPITATION; EMBRYONIC STEM CELLS; HUMANS; MICE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOFTWARE;

EID: 67650672176     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-59     Document Type: Article

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