Mutations of key hydrophobic surface residues of 11β-hydroxysteroid dehydrogenase type 1 increase solubility and monodispersity in a bacterial expression system

Protein Science

Volumn 18, Issue 7, 2009, Pages 1552-1563

  Lawson, Alexander J ^a   Walker, Elizabeth A ^a   White, Scott A ^a   Dafforn, Timothy R ^a   Stewart, Paul M ^a   Ride, Jonathan P ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

11 hydroxysteroid dehydrogenase type 1 (11 HSD1); Hydrophobicity; Mutation; Recombinant protein expression

Indexed keywords

11BETA HYDROXYSTEROID DEHYDROGENASE 1; OXIDOREDUCTASE; RECOMBINANT ENZYME; STEROID;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE MUTATION; GUINEA PIG; HYDROPHOBICITY; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; SOLUBILITY; SURFACE PROPERTY; ULTRACENTRIFUGATION; WILD TYPE;

11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 1; AMINO ACID SEQUENCE; ANIMALS; AREA UNDER CURVE; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GUINEA PIGS; HUMANS; HYDROPHOBICITY; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SOLUBILITY; SURFACE PROPERTIES; ULTRACENTRIFUGATION;

BACTERIA (MICROORGANISMS); CAVIA;

EID: 67650047734     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.150     Document Type: Article

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