Structural role of conserved Asn179 in the short-chain dehydrogenase/reductase scaffold

Biochemical and Biophysical Research Communications

Volumn 289, Issue 3, 2001, Pages 712-717

  Filling, Charlotta ^a   Nordling, Erik ^a   Benach, Jordi ^a   Berndt, Kurt D ^a,b   Ladenstein, Rudolf ^a   Jörnvall, Hans ^a   Oppermann, Udo ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b SÖDERTÖRN UNIVERSITY   (Sweden)

Author keywords

11 hydroxysteroid dehydrogenase; Glycosylation; Hydroxysteroid dehydrogenase; Short chain dehydrogenase reductase; Site directed mutagenesis; Three dimensional structure

Indexed keywords

11BETA HYDROXYSTEROID DEHYDROGENASE; 3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; ASPARAGINE; GUANIDINE; OXIDOREDUCTASE;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME DENATURATION; ENZYME GLYCOSYLATION; ENZYME INACTIVATION; ENZYME STRUCTURE; GENETIC CONSERVATION; HYDROGEN BOND; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN FOLDING; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 0035824597     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.6032     Document Type: Article

References (21)

1. Short-chain dehydrogenases/reductases (SDR)
2. SDR and MDR: Completed genome sequences show these protein families to be large, of old origin, and of complex nature
3. 11 Beta-hydroxysteroid dehydrogenase and the short-chain alcohol dehydrogenase (SCAD) superfamily
4. Forms and functions of human SDR enzymes
5. The fascinating complexities of steroid-binding enzymes
6. Structure and function of steroid dehydrogenases involved in hypertension, fertility, and cancer
7. Metabolic conversion as a pre-receptor control mechanism for lipophilic hormones
8. Structure-function relationships and molecular genetics of the 3 beta-hydroxysteroid dehydrogenase gene family
9. Structure-function relationships of SDR hydroxysteroid dehydrogenases
10. Active site directed mutagenesis of 3β/17β-hydroxysteroid dehydrogenase establishes differential effects on short-chain dehydrogenase/reductase reactions
11. Drosophila alcohol dehydrogenase: Evaluation of Ser139 site-directed mutants
12. The refined crystal structure of Drosophila lebanonensis alcohol dehydrogenase at 1.9 Å resolution
13. 11β-Hydroxysteroid dehydrogenase
14. Mutations in putative glycosylation sites of rat 11β-hydroxysteroid dehydrogenase affect enzymatic activity
15. Crystallization and crystal packing of recombinant 3 (or 17) β-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996
16. The catalytic reaction and inhibition mechanism of Drosophila alcohol dehydrogenase: Observation of an enzyme-bound NAD-ketone adduct at 1.4 Å resolution by X-ray crystallography
17. Functional expression, characterization, and purification of the catalytic domain of human 11-beta-hydroxysteroid dehydrogenase type 1
18. Human 11beta-hydroxysteroid dehydrogenase type 1 is enzymatically active in its nonglycosylated form
19. Expression of 11 beta-hydroxysteroid dehydrogenase using recombinant vaccinia virus
20. Molecular mechanisms of estrogen recognition and 17-keto reduction by human 17β-hydroxysteroid dehydrogenase 1
21. +-dependent 15-hydroxyprostaglandin dehydrogenase