High-level production and optimization of monodispersity of 11β-hydroxysteroid dehydrogenase type 1

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1700, Issue 2, 2004, Pages 199-207

  Elleby, Björn ^a   Svensson, Stefan ^a   Wu, Xiaoqiu ^b   Stefansson, Karin ^a   Nilsson, Joakim ^a   Hallén, Dan ^a   Oppermann, Udo ^b   Abrahmsén, Lars ^a  

^a BIOVITRUM AB   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

11 H1; 11 Hydroxysteroid dehydrogenase; 11 hydroxysteroid dehydrogenase; endoplasmic reticulum; Er; GroEL ES chaperonin; guanidine hydrochloride; Guhcl; Heterologous production; Inhibitor; Oligomerization state; Purification; Sdr

Indexed keywords

11BETA HYDROXYSTEROID DEHYDROGENASE; 11BETA HYDROXYSTEROID DEHYDROGENASE TYPE 1; BACTERIAL PROTEIN; CHAPERONIN; DETERGENT; DIMER; ENZYME INHIBITOR; OLIGOMER; POLYMER; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CHROMATOGRAPHY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE OVEREXPRESSION; GUINEA PIG; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN SYNTHESIS; SCREENING; SOLUBILITY; SOLUBILIZATION; TITRIMETRY; ULTRACENTRIFUGATION;

11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 1; ANIMALS; CLONING, MOLECULAR; DIMERIZATION; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GROEL PROTEIN; GUINEA PIGS; HUMANS; NADP; RATS; RECOMBINANT PROTEINS; SOLUBILITY;

CAVIA PORCELLUS; SUS SCROFA;

EID: 3242678242     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2004.05.003     Document Type: Article

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