Impact of the comet assay in radiobiology

Mutation Research - Reviews in Mutation Research

Volumn 681, Issue 1, 2009, Pages 13-23

  Olive, Peggy L ^a  

^a BRITISH COLUMBIA CANCER RESEARCH CENTRE   (Canada)

Author keywords

Comet assay; DNA strand breaks; Ionizing radiation

Indexed keywords

DNA BASE; RADIOISOTOPE;

APOPTOSIS; BYSTANDER EFFECT; COMET ASSAY; DNA DAMAGE; DNA DETERMINATION; DNA FINGERPRINTING; DNA REPAIR; DOUBLE STRANDED DNA BREAK; FLUORESCENCE IN SITU HYBRIDIZATION; FOOD IRRADIATION; HUMAN; HYPOXIC CELL; IONIZING RADIATION; IRRADIATION; NONHUMAN; PRIORITY JOURNAL; RADIATION DOSE; RADIATION EXPOSURE; RADIATION PROTECTION; RADIOBIOLOGY; REVIEW; SOLID TUMOR;

ANIMALS; APOPTOSIS; CELL HYPOXIA; COMET ASSAY; DNA; DNA BREAKS, DOUBLE-STRANDED; DNA DAMAGE; DNA REPAIR; HISTORY, 20TH CENTURY; HISTORY, 21ST CENTURY; HUMANS; NEOPLASMS; RADIOBIOLOGY;

EID: 62149140605     PISSN: 13835742     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrrev.2007.11.001     Document Type: Review

References (124)

1. Bedford J.S., and Dewey W.C. Radiation Research Society. 1952-2002. Historical and current highlights in radiation biology: has anything important been learned by irradiating cells?. Radiat. Res. 158 (2002) 251-291
2. McGrath R.A., and Williams R.W. Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces. Nature 212 (1966) 534-535
3. Lett J.T., Caldwell I., Dean C.J., and Alexander P. Rejoining of X-ray induced breaks in the DNA of leukaemia cells. Nature 214 (1967) 790-792
4. Elkind M.M., and Kamper C. Two forms of repair of DNA in mammalian cells following irradiation. Biophys. J. 10 (1970) 237-245
5. Kohn K.W., and Grimek-Ewig. Alkaline elution analysis, a new approach to the study of DNA single-strand interruptions in cells. Cancer Res. 33 (1973) 1849-1853
6. Ahnstrom G. Techniques to measure DNA single-strand breaks in cells: a review. Int. J. Radiat. Biol. 54 (1988) 695-707
7. Rydberg B. The rate of strand separation in alkali of DNA of irradiated mammalian cells. Radiat. Res. 61 (1975) 274-287
8. Hutchinson F. On the measurement of DNA double-strand breaks by neutral elution. Radiat. Res. 120 (1989) 182-186
9. Wright W.D., Lagroye I., Zhang P., Malyapa R.S., and Roti J.L. Cytometric methods to analyze ionizing-radiation effects. Methods Cell Biol. 64 (2001) 251-268
10. Cook P.R., and Brazell I.A. Supercoils in human DNA. J. Cell Sci. 19 (1975) 261-279
11. Erickson L.C., Osieka R., Sharkey N.A., and Kohn K.W. Measurement of DNA damage in unlabeled mammalian cells analyzed by alkaline elution and a fluorometric DNA assay. Anal. Biochem. 106 (1980) 169-174
12. Blocher D., Einspenner M., and Zajackowski J. CHEF electrophoresis, a sensitive technique for the determination of DNA double-strand breaks. Int. J. Radiat. Biol. 56 (1989) 437-448
13. Birnboim H.C., and Jevcak J.J. Fluorometric method for rapid detection of DNA strand breaks in human white blood cells produced by low doses of radiation. Cancer Res. 41 (1981) 1889-1892
14. Roti J.L., and Wright W.D. Visualization of DNA loops in nucleoids from HeLa cells: assays for DNA damage and repair. Cytometry 8 (1987) 461-467
15. Okayasu R., Bloecher D., and Iliakis G. Variation through the cell cycle in the dose-response of DNA neutral filter elution in X-irradiated synchronous CHO-cells. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 53 (1988) 729-747
16. Rydberg B. Detection of DNA strand breaks in single cells using flow cytometry. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 46 (1984) 521-527
17. Ostling O., and Johanson K.J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123 (1984) 291-298
18. Singh N.P., McCoy M.T., Tice R.R., and Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175 (1988) 184-191
19. Olive P.L. Cell proliferation as a requirement for development of the contact effect in Chinese hamster V79 spheroids. Radiat. Res. 117 (1989) 79-92
20. Olive P.L. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol. 75 (1999) 395-405
21. Ostling O., and Johanson K.J. Bleomycin, in contrast to gamma irradiation, induces extreme variation of DNA strand breakage from cell to cell. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 52 (1987) 683-691
22. Luo C., Johnston P.J., MacPhail S., Banáth J.P., Oloumi A., and Olive P.L. Cell fusion studies to examine the mechanism for etoposide resistance in Chinese hamster V79 spheroids. Exp. Cell Res. 243 (1998) 282-289
23. Olive P.L., Durand R.E., Jackson S.M., LeRiche J.C., Luo C., Ma R., McLaren D.B., Aquino-Parsons C., Thomson T.A., and Trotter T. The comet assay in clinical practice. Acta Oncol. 38 (1999) 839-844
24. Dorie M.J., Kovacs M.S., Gabalski E.C., Adam M., Le Q.T., Bloch D.A., Pinto H.A., Terris D.J., and Brown J.M. DNA damage measured by the comet assay in head and neck cancer patients treated with tirapazamine. Neoplasia 1 (1999) 461-467
25. Garcia O., and Mandina T. DNA damage evaluated by the comet assay in lymphocytes of children with 137Cs internal contamination caused by the Chernobyl accident. Mutat. Res. 565 (2005) 191-197
26. Olive P.L., Wlodek D., and Banáth J.P. DNA double-strand breaks measured in individual cells subjected to gel electrophoresis. Cancer Res. 51 (1991) 4671-4676
27. Saul R.L., and Ames B.N. Background levels of DNA damage in the population. Basic Life Sci. 38 (1986) 529-535
28. Loft S., and Poulsen H.E. Cancer risk and oxidative DNA damage in man. J. Mol. Med. 74 (1996) 297-312
29. Olive P.L. The role of DNA single- and double-strand breaks in cell killing by ionizing radiation. Radiat. Res. 150 (1998) S42-S51
30. Bedford J.S., and Cornforth M.N. Relationship between the recovery from sublethal X-ray damage and the rejoining of chromosome breaks in normal human fibroblasts. Radiat. Res. 111 (1987) 406-423
31. Frankenberg-Schwager M., and Frankenberg D. DNA double-strand breaks: their repair and relationship to cell killing in yeast. Int. J. Radiat. Biol. 58 (1990) 569-575
32. Gradzka I., and Iwanenko T. A non-radioactive, PFGE-based assay for low levels of DNA double-strand breaks in mammalian cells. DNA Repair (Amst.) 4 (2005) 1129-1139
33. Eastham A.M., Marples B., Kiltie A.E., Orton C.J., and West C.M. Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. Br. J. Cancer 79 (1999) 1366-1371
34. Olive P.L., and Durand R.E. Heterogeneity in DNA damage using the comet assay. Cytometry A 66 (2005) 1-8
35. Rydberg B. Radiation-induced heat-labile sites that convert into DNA double-strand breaks. Radiat. Res. 153 (2000) 805-812
36. Olive P.L., and Banáth J.P. The comet assay: a method to measure DNA damage in individual cells. Nat. Protocols 1 (2006) 23-29
37. Testard I., and Sabatier L. Assessment of DNA damage induced by high-LET ions in human lymphocytes using the comet assay. Mutat. Res. 448 (2000) 105-115
38. Stenerlow B., Hoglund E., Carlsson J., and Blomquist E. Rejoining of DNA fragments produced by radiations of different linear energy transfer. Int. J. Radiat. Biol. 76 (2000) 549-557
39. Ismail I.H., Nystrom S., Nygren J., and Hammarsten O. Activation of ataxia telangiectasia mutated by DNA strand break-inducing agents correlates closely with the number of DNA double strand breaks. J. Biol. Chem. 280 (2005) 4649-4655
40. Sedelnikova O.A., Pilch D.R., Redon C., and Bonner W.M. Histone H2AX in DNA damage and repair. Cancer Biol. Ther. 2 (2003) 233-235
41. Vaupel P., Kelleher D.K., and Hockel M. Oxygen status of malignant tumors: pathogenesis of hypoxia and significance for tumor therapy. Semin. Oncol. 28 (2001) 29-35
42. Stone H.B., Brown J.M., Phillips T.L., and Sutherland R.M. Oxygen in human tumors: correlations between methods of measurement and response to therapy. Summary of a Workshop Held November 19-20, 1992. National Cancer Institute, Bethesda, Maryland. Radiat. Res. 136 (1993) 422-434
43. Olive P.L., Vikse C., and Durand R.E. Hypoxic fractions measured in murine tumours and normal tissues using the comet assay. Int. J. Radiat. Oncol. Biol. Phys. 29 (1994) 487-491
44. Hu Q., Kavanagh M.C., Newcombe D., and Hill R.P. Detection of hypoxic fractions in murine tumors by comet assay: comparison with other techniques. Radiat. Res. 144 (1995) 266-275
45. Olive P.L., and Durand R.E. Detection of hypoxic cells in a murine tumor with the use of the comet assay. J. Natl. Cancer Inst. 84 (1992) 707-711
46. Olive P.L., Horsman M.R., Grau C., and Overgaard J. Detection of hypoxic cells in a C3H mouse mammary carcinoma using the comet assay. Br. J. Cancer 76 (1997) 694-699
47. Olive P.L., Durand R.E., LeRiche J.C., Olivotto I.A., and Jackson S.M. Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers. Cancer Res. 53 (1993) 733-736
48. Wang J., Klem J., Wyrick J.B., Ozawa T., Cunningham E., Golinveaux J., Allen M.J., Lamborn K.R., and Deen D.F. Detection of hypoxia in human brain tumor xenografts using a modified comet assay. Neoplasia 5 (2003) 288-296
49. Partridge S.E., Aquino-Parsons C., Luo C., Green A., and Olive P.L. A pilot study comparing intratumoral oxygenation using the comet assay following 2.5% and 5% carbogen and 100% oxygen. Int. J. Radiat. Oncol. Biol. Phys. 49 (2001) 575-580
50. McLaren D.B., Pickles T., Thomson T., and Olive P.L. Impact of nicotinamide on human tumour hypoxic fraction measured using the comet assay. Radiother. Oncol. 45 (1997) 175-182
51. Olive P.L., Banath J.P., and Aquino-Parsons C. Measuring hypoxia in solid tumours-is there a gold standard?. Acta Oncol. 40 (2001) 917-923
52. Macdonald G., Kondor N., Yousefi V., Green A., Wong F., and Aquino-Parsons C. Reduction of carboxyhaemoglobin levels in the venous blood of cigarette smokers following the administration of carbogen. Radiother. Oncol. 73 (2004) 367-371
53. Olive P.L., Durand R.E., Raleigh J.A., Luo C., and quino-Parsons C. Comparison between the comet assay and pimonidazole binding for measuring tumour hypoxia. Br. J. Cancer 83 (2000) 1525-1531
54. Kavanagh M.C., Tsang V., Chow S., Koch C., Hedley D., Minkin S., and Hill R.P. A comparison in individual murine tumors of techniques for measuring oxygen levels. Int. J. Radiat. Oncol. Biol. Phys. 44 (1999) 1137-1146
55. Olive P.L. Detection of hypoxia by measurement of DNA damage in individual cells from spheroids and murine tumours exposed to bioreductive drugs. I. Tirapazamine. Br. J. Cancer 71 (1995) 529-536
56. Olive P.L. Detection of hypoxia by measurement of DNA damage in individual cells from spheroids and murine tumours exposed to bioreductive drugs. II. RSU 1069. Br. J. Cancer 71 (1995) 537-542
57. Tercel M., Lee A.E., Hogg A., Anderson R.F., Lee H.H., Siim B.G., Denny W.A., and Wilson W.R. Hypoxia-selective antitumor agents. 16. Nitroarylmethyl quaternary salts as bioreductive prodrugs of the alkylating agent mechlorethamine. J. Med. Chem. 44 (2001) 3511-3522
58. Hu Q., and Hill R.P. Radiosensitivity, apoptosis and repair of DNA double-strand breaks in radiation-sensitive Chinese hamster ovary cell mutants treated at different dose rates. Radiat. Res. 146 (1996) 636-645
59. Wojewodzka M., Kruszewski M., Buraczewska I., Xu W., Massuda E., Zhang J., and Szumiel I. Sirtuin inhibition increases the rate of non-homologous end-joining of DNA double strand breaks. Acta Biochim. Pol. 54 (2007) 63-69
60. Ward A.J., Olive P.L., Burr A.H., and Rosin M.P. Response of fibroblast cultures from ataxia-telangiectasia patients to reactive oxygen species generated during inflammatory reactions. Environ. Mol. Mutagen. 24 (1994) 103-111
61. Alapetite C. Individual radiosensitivity and DNA repair proficiency: the value of the comet assay. Cancer Radiother. 2 (1998) 534-540
62. Kuhne M., Riballo E., Rief N., Rothkamm K., Jeggo P.A., and Lobrich M. A double-strand break repair defect in ATM-deficient cells contributes to radiosensitivity. Cancer Res. 64 (2004) 500-508
63. Kasten-Pisula U., Tastan H., and Dikomey E. Huge differences in cellular radiosensitivity due to only very small variations in double-strand break repair capacity. Int. J. Radiat. Biol. 81 (2005) 409-419
64. Wang H., Zeng Z.C., Bui T.A., Sonoda E., Takata M., Takeda S., and Iliakis G. Efficient rejoining of radiation-induced DNA double-strand breaks in vertebrate cells deficient in genes of the RAD52 epistasis group. Oncogene 20 (2001) 2212-2224
65. Nocentini S. Comet assay analysis of repair of DNA strand breaks in normal and deficient human cells exposed to radiations and chemicals. Evidence for a repair pathway specificity of DNA ligation. Radiat. Res. 144 (1995) 170-180
66. Trenz K., Schutz P., and Speit G. Radiosensitivity of lymphoblastoid cell lines with a heterozygous BRCA1 mutation is not detected by the comet assay and pulsed field gel electrophoresis. Mutagenesis 20 (2005) 131-137
67. Olive P.L., Banáth J.P., and MacPhail S. Lack of a correlation between radiosensitivity and DNA double-strand break induction or rejoining in six human tumor cell lines. Cancer Res. 54 (1994) 3939-3946
68. Olive P.L., and Banáth J.P. Induction and rejoining of radiation-induced DNA single-strand breaks: "tail moment" as a function of position in the cell cycle. Mutat. Res. 294 (1993) 275-283
69. Banath J.P., Sinnott L., Larrivee B., MacPhail S.H., and Olive P.L. Growth of V79 cells as xenograft tumors promotes multicellular resistance but does not increase spontaneous or radiation-induced mutant frequency. Radiat. Res. 164 (2005) 733-744
70. Myllyperkio M.H., Koski T.R., Vilpo L.M., and Vilpo J.A. Gamma-irradiation-induced DNA single- and double-strand breaks and their repair in chronic lymphocytic leukemia cells of variable radiosensitivity. Hematol. Cell Ther. 41 (1999) 95-103
71. Myllyperkio M.H., Koski T.R., Vilpo L.M., and Vilpo J.A. Kinetics of excision repair of UV-induced DNA damage, measured using the comet assay. Mutat. Res. 448 (2000) 1-9
72. Hazane F., Sauvaigo S., Douki T., Favier A., and Beani J.C. Age-dependent DNA repair and cell cycle distribution of human skin fibroblasts in response to UVA irradiation. J. Photochem. Photobiol. B 82 (2006) 214-223
73. Mayer P.J., Lange C.S., Bradley M.O., and Nichols W.W. Age-dependent decline in rejoining of X-ray-induced DNA double-strand breaks in normal human lymphocytes 7. Mutat. Res. 219 (1989) 95-100
74. Singh N.P., Ogburn C.E., Wolf N.S., van B.G., and Martin G.M. DNA double-strand breaks in mouse kidney cells with age. Biogerontology 2 (2001) 261-270
75. Yu T., MacPhail S.H., Banath J.P., Klokov D., and Olive P.L. Endogenous expression of phosphorylated histone H2AX in tumors in relation to DNA double-strand breaks and genomic instability. DNA Repair (Amst.) 5 (2006) 935-946
76. Singh N.P., Danner D.B., Tice R.R., Brant L., and Schneider E.L. DNA damage and repair with age in individual human lymphocytes. Mutat. Res. 237 (1990) 123-130
77. Eriksson S., Nygren J., and Ahnstrom G. Matrix association of early- and late-replicating chromatin studied by single-cell electrophoresis. Biochim. Biophys. Acta 1590 (2002) 103-108
78. Flentje M., Asadpour B., Latz D., and Weber K.J. Sensitivity of neutral filter elution but not PFGE can be modified by non-dsb chromatin damage. Int. J. Radiat. Biol. 63 (1993) 715-724
79. Bradley M.O., and Kohn K.W. X-ray induced DNA double-strand break production and repair in mammalian cells as measured by neutral filter elution. Nucleic Acids Res. 7 (1979) 793-804
80. Jones I.M., Thomas C.B., Xi T., Mohrenweiser H.W., and Nelson D.O. Exploration of methods to identify polymorphisms associated with variation in DNA repair capacity phenotypes. Mutat. Res. 616 (2007) 213-220
81. Cornetta T., Festa F., Testa A., and Cozzi R. DNA damage repair and genetic polymorphisms: assessment of individual sensitivity and repair capacity. Int. J. Radiat. Oncol. Biol. Phys. 66 (2006) 537-545
82. Cassoni A.M., McMillan T.J., Peacock J.H., and Steel G.G. Differences in the level of DNA double-strand breaks in human tumour cell lines following low dose-rate irradiation. Eur. J. Cancer 28A (1992) 1610-1614
83. Muller W.U., Bauch T., Stuben G., Sack H., and Streffer C. Radiation sensitivity of lymphocytes from healthy individuals and cancer patients as measured by the comet assay. Radiat. Environ. Biophys. 40 (2001) 83-89
84. Popanda O., Ebbeler R., Twardella D., Helmbold I., Gotzes F., Schmezer P., Thielmann H.W., von F.D., Haase W., Sautter-Bihl M.L., Wenz F., Bartsch H., and Chang-Claude J. Radiation-induced DNA damage and repair in lymphocytes from breast cancer patients and their correlation with acute skin reactions to radiotherapy. Int. J. Radiat. Oncol. Biol. Phys. 55 (2003) 1216-1225
85. Alapetite C., Thirion P., de la R.A., Cosset J.M., and Moustacchi E. Analysis by alkaline comet assay of cancer patients with severe reactions to radiotherapy: defective rejoining of radioinduced DNA strand breaks in lymphocytes of breast cancer patients. Int. J. Cancer 83 (1999) 83-90
86. Muller W.U., Bauch T., Streffer C., and Von M.D. Does radiotherapy affect the outcome of the comet assay?. Br. J. Radiol. 75 (2002) 608-614
87. Djuzenova C.S., Muhl B., Fehn M., Oppitz U., Muller B., and Flentje M. Radiosensitivity in breast cancer assessed by the comet and micronucleus assays. Br. J. Cancer 94 (2006) 1194-1203
88. Wang W.D., Chen Z.T., Li D.Z., Cao Z.H., Sun S.L., Pu P., and Chen X.P. Correlation between DNA repair capacity in lymphocytes and acute side effects to skin during radiotherapy in nasopharyngeal cancer patients. Clin. Cancer Res. 11 (2005) 5140-5145
89. Bentzen S.M. Preventing or reducing late side effects of radiation therapy: radiobiology meets molecular pathology. Nat. Rev. Cancer 6 (2006) 702-713
90. Banath J.P., MacPhail S.H., and Olive P.L. Radiation sensitivity, H2AX phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines. Cancer Res. 64 (2004) 7144-7149
91. Purschke M., Kasten-Pisula U., Brammer I., and Dikomey E. Human and rodent cell lines showing no differences in the induction but differing in the repair kinetics of radiation-induced DNA base damage. Int. J. Radiat. Biol. 80 (2004) 29-38
92. Moneef M.A., Sherwood B.T., Bowman K.J., Kockelbergh R.C., Symonds R.P., Steward W.P., Mellon J.K., and Jones G.D. Measurements using the alkaline comet assay predict bladder cancer cell radiosensitivity. Br. J. Cancer 89 (2003) 2271-2276
93. McKeown S.R., Robson T., Price M.E., Ho E.T., Hirst D.G., and Kelvey-Martin V.J. Potential use of the alkaline comet assay as a predictor of bladder tumour response to radiation. Br. J. Cancer 89 (2003) 2264-2270
94. McKelvey-Martin V.J., Ho E.T., McKeown S.R., Johnston S.R., McCarthy P.J., Rajab N.F., and Downes C.S. Emerging applications of the single cell gel electrophoresis (Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells. Mutagenesis 13 (1998) 1-8
95. Dunne A.L., Price M.E., Mothersill C., McKeown S.R., Robson T., and Hirst D.G. Relationship between clonogenic radiosensitivity, radiation-induced apoptosis and DNA damage/repair in human colon cancer cells. Br. J. Cancer 89 (2003) 2277-2283
96. Brammer I., Zoller M., and Dikomey E. Relationship between cellular radiosensitivity and DNA damage measured by comet assay in human normal, NBS and AT fibroblasts. Int. J. Radiat. Biol. 77 (2001) 929-938
97. Olive P.L. DNA organization affects cellular radiosensitivity and detection of initial DNA strand breaks. Int. J. Radiat. Biol. 62 (1992) 389-396
98. Rajab N.F., McKenna D.J., Diamond J., Williamson K., Hamilton P.W., and Kelvey-Martin V.J. Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. Cytometry A 69 (2006) 1077-1085
99. Chapman J.D., Stobbe C.C., and Matsumoto Y. Chromatin compaction and tumor cell radiosensitivity at 2 gray. Am. J. Clin. Oncol. 24 (2001) 509-515
100. Gurska S., Farkasova T., and Gabelova A. Radiosensitivity of cervical cancer cell lines: the impact of polymorphisms in DNA repair genes. Neoplasma 54 (2007) 195-201
101. Bergqvist M., Brattstrom D., Stalberg M., Vaghef H., Brodin O., and Hellman B. Evaluation of radiation-induced DNA damage and DNA repair in human lung cancer cell lines with different radiosensitivity using alkaline and neutral single cell gel electrophoresis. Cancer Lett. 133 (1998) 9-18
102. Frankenberg D., Frankenberg-Schwager M., and Harbich R. Interpretation of the shape of survival curves in terms of induction and repair/misrepair of DNA double-strand breaks. Br. J. Cancer Suppl 6 (1984) 233-238
103. Collins A.R., Duthie S.J., and Dobson V.L. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis 14 (1993) 1733-1735
104. Collins A.R., Dusinska M., Gedik C.M., and Stetina R. Oxidative damage to DNA: do we have a reliable biomarker?. Environ. Health Perspect. 104 Suppl. 3 (1996) 465-469
105. Wallace S.S. Enzymatic processing of radiation-induced free radical damage in DNA. Radiat. Res. 150 (1998) S60-S79
106. Pouget J.P., Ravanat J.L., Douki T., Richard M.J., and Cadet J. Measurement of DNA base damage in cells exposed to low doses of gamma-radiation: comparison between the HPLC-EC and comet assays. Int. J. Radiat. Biol. 75 (1999) 51-58
107. Banath J.P., Wallace S.S., Thompson J., and Olive P.L. Radiation-induced DNA base damage detected in individual aerobic and hypoxic cells with endonuclease III and formamidopyrimidine-glycosylase. Radiat. Res. 151 (1999) 550-558
108. Ponnaiya B., Jenkins-Baker G., Brenner D.J., Hall E.J., Randers-Pehrson G., and Geard C.R. Biological responses in known bystander cells relative to known microbeam-irradiated cells. Radiat. Res. 162 (2004) 426-432
109. Przybyszewski W.M., Widel M., Szurko A., Lubecka B., Matulewicz L., Maniakowski Z., Polaniak R., Birkner E., and Rzeszowska-Wolny J. Multiple bystander effect of irradiated megacolonies of melanoma cells on non-irradiated neighbours. Cancer Lett. 214 (2004) 91-102
110. Morgan W.F., Hartmann A., Limoli C.L., Nagar S., and Ponnaiya B. Bystander effects in radiation-induced genomic instability. Mutat. Res. 504 (2002) 91-100
111. Gault N., Rigaud O., Poncy J.L., and Lefaix J.L. Biochemical alterations in human cells irradiated with alpha particles delivered by macro- or microbeams. Radiat. Res. 167 (2007) 551-562
112. Muller A.C., Pigorsch S., Beyer C., Lautenschlager C., and Dunst J. Radioprotective effects of amifostine in vitro and in vivo measured with the comet assay. Strahlenther. Onkol. 180 (2004) 517-525
113. Srinivasan M., Sudheer A.R., Pillai K.R., Kumar P.R., Sudhakaran P.R., and Menon V.P. Influence of ferulic acid on gamma-radiation induced DNA damage, lipid peroxidation and antioxidant status in primary culture of isolated rat hepatocytes. Toxicology 228 (2006) 249-258
114. Olive P.L., Frazer G., and Banáth J.P. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat. Res. 136 (1993) 130-136
115. Olive P.L., and Banáth J.P. Sizing highly fragmented DNA in individual apoptotic cells using the comet assay and a DNA crosslinking agent. Exp. Cell Res. 221 (1995) 19-26
116. Morley N., Rapp A., Dittmar H., Salter L., Gould D., Greulich K.O., and Curnow A. UVA-induced apoptosis studied by the new apo/necro-Comet-assay which distinguishes viable, apoptotic and necrotic cells. Mutagenesis 21 (2006) 105-114
117. McKenna D.J., Rajab N.F., McKeown S.R., McKerr G., and Kelvey-Martin V.J. Use of the comet-FISH assay to demonstrate repair of the TP53 gene region in two human bladder carcinoma cell lines. Radiat. Res. 159 (2003) 49-56
118. Gajendiran N., Tanaka K., and Kamada N. Comet assay to sense neutron 'fingerprint'. Mutat. Res. 452 (2000) 179-187
119. Wada S., Kobayashi Y., Funayama T., Natsuhori M., Ito N., and Yamamoto K. Detection of DNA damage in individual cells induced by heavy-ion irradiation with an non-denaturing comet assay. J. Radiat. Res. (Tokyo) 43 Suppl. (2002) S153-S156
120. Prise K.M., Ahnstrom G., Belli M., Carlsson J., Frankenberg D., Kiefer J., Lobrich M., Michael B.D., Nygren J., Simone G., and Stenerlow B. A review of dsb induction data for varying quality radiations. Int. J. Radiat. Biol. 74 (1998) 173-184
121. Cerda H., Delincee H., Haine H., and Rupp H. The DNA 'comet assay' as a rapid screening technique to control irradiated food. Mutat. Res. 375 (1997) 167-181
122. Olive P.L., and Banáth J.P. Detection of DNA double-strand breaks through the cell cycle after exposure to X-rays, bleomycin, etoposide and 125IdUrd. Int. J. Radiat. Biol. 64 (1993) 349-358
123. LeMotte P.K., and Little J.B. DNA damage induced in human diploid cells by decay of incorporated radionuclides. Cancer Res. 44 (1984) 1337-1342
124. Zheng H., and Olive P.L. Reduction of tumor hypoxia and inhibition of DNA repair by nicotinamide after irradiation of SCCVII murine tumors and normal tissues. Cancer Res. 56 (1996) 2801-2808