Use of the comet-FISH assay to demonstrate repair of the TP53 gene region in two human bladder carcinoma cell lines

Radiation Research

Volumn 159, Issue 1, 2003, Pages 49-56

  McKenna, Declan J ^a   Rajab, Nor F ^a   McKeown, Stephanie R ^a   McKerr, George ^a   McKelvey Martin, Valerie J ^a  

^a UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLADDER CARCINOMA; CANCER CELL CULTURE; CELL STRAIN RT112; CELL STRAIN RT4; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; FLUORESCENCE IN SITU HYBRIDIZATION; GAMMA IRRADIATION; GENE; GENE LOCATION; GENOME; HUMAN; HUMAN CELL; PRIORITY JOURNAL; TP53 GENE;

COMET ASSAY; DNA DAMAGE; DNA REPAIR; GAMMA RAYS; GENES, P53; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; TUMOR CELLS, CULTURED; URINARY BLADDER NEOPLASMS;

EID: 0037228807     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/0033-7587(2003)159[0049:UOTCFA]2.0.CO;2     Document Type: Article

References (25)

1. N. P. Singh, M. T McCoy, R. R. Tice and E. L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175, 184-191 (1988).
2. D. W. Fairbairn, P. L. Olive and K, L. O'Neill, The comet assay: A comprehensive review. Mutat, Res. 339, 37-59 (1995).
3. R. R. Tice, E. Agurell, D. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J. C. Ryu and Y. F. Sasaki, Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ, Mol. Mutagen. 35, 206-221 (2000).
4. S. J. Santos, N. P. Singh and A. T. Natarajan, Fluorescence in situ hybridization with comets. Exp. Cell Res. 232, 407-411 (1997).
5. V. J. McKelvey-Martin, E. T. S. Ho, S. R. McKeown, S. R. Johnston, P. J. McCarthy, N. F. Rajab and C. S. Downes, Emerging applications of the single cell gel electrophoresis (comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization comets for the identification of damaged and repaired DNA sequences in individual cells. Mutagenesis 13, 1-8 (1998).
6. C. Bock, A. Rapp, H. Dittmar, S. Monajembashi and K-O. Greulich, Localization of specific sequences and DNA single strand breaks in individual UV-A irradiated human lymphocytes by COMET FISH. Prog. Biochem. Optics SPIE 3568, 207-217 (1999).
7. M. Menke, K. J. Angelis and I. Schubert, Detection of specific DNA lesions by a combination of comet assay and FISH in plants. Environ. Mol. Mutagen. 35, 132-138 (2000).
8. N. F. Rajab and V. J. McKelvey-Martin, Preferential rejoining of γ radiation induced DNA strand breaks in the p53 domain of J82 bladder carcinoma cells. Mutagenesis 14, 649-650 (1999).
9. B. Calabretta, L. Kaczmarek, L. Selleri, G. Torelli, P. M. Ming, S. C. Ming and W. E. Mercer, Growth-dependent expression of human Mr 53,000 tumour antigen messenger RNA in normal and neoplastic cells. Cancer Res. 46, 5738-5742 (1986).
10. B. C. McKay, M. Ljungman and A. J. Rainbow, Potential roles for p53 in nucleotide excision repair. Carcinogenesis 20, 1389-1396 (1999).
11. M. K. Evans, B. G. Taffe, C. C. Harris and V. A. Bohr, DNA strand bias in the repair of the p53 gene in normal human and xeroderma pigmentosum group C fibroblasts. Cancer Res. 53, 5377-5381 (1993).
12. J. M. Ford, L. Lommel and P. C. Hanawalt, Preferential repair of ultraviolet light-induced DNA damage in the transcribed strand of the human p53 gene. Mol. Carcinog. 10, 105-109 (1994).
13. S. A. Leadon and P. K. Cooper, Preferential repair of ionizing radiation-induced damage in the transcribed strand of an active human gene is defective in Cockayne syndrome. Proc. Natl. Acad. Sci. USA 90, 10499-10503 (1993).
14. P. K. Cooper, T Nouspikel, S. G. Clarkson and S. A. Leadon, Defective transcription-coupled repair of oxidative base damage in Cockayne syndrome patients from XP group G. Science 275, 990-993 (1997).
15. A. May and V. A. Bohr, Gene-specific repair of gamma-ray-induced DNA strand breaks in colon cancer cells: No coupling to transcription and no removal from the mitochondrial genome. Biochem. Biophys. Res. Commun. 269, 433-437 (2000).
16. V. A. Bohr, C. A. Smith, D. S. Okumoto and P. C. Hanawalt, DNA repair in an active gene: Removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall. Cell 40, 359-369 (1985).
17. I. Mellon, V. A. Bohr, C. A. Smith and P. C. Hanawalt, Preferential DNA repair of an active gene in human cells. Proc. Natl. Acad. Sci. USA 83, 8878-8882 (1986).
18. J. Venema, Z. Bartosova, A. T Natarajan, A. A. van Zeeland and L. H. Mullenders, Transcription affects the rate but not the extent of repair of cyclobutane pyrimidine dimers in the human adenosine deaminase gene. J. Biol. Chem. 267, 8852-8856 (1992).
19. H. J. Ruven, C. M. Seelen, P. H. Lohman, H. van Kranen, A. A. van Zeeland and L. H. Mullenders, Strand-specific removal of cyclobutane pyrimidine dimers from the p53 gene in the epidermis of UVB-irradiated hairless mice. Oncogene 9, 3427-3432 (1994).
20. C. M. Chresta, J. R. Masters and J. A. Hickman, Hypersensitivity of human testicular tumors to etoposide-induced apoptosis is associated with functional p53 and a high Bax:Bcl-2 ratio, Cancer Res. 56, 1834-1841 (1996).
21. H. M. Warenius, M. Jones, T Gorman, R. McLeish, L. Seabra, R. Barraclough and P. Rudland, Combined RAF1 protein expression and p53 mutational status provides a strong predictor of cellular radio-sensitivity. Br. J. Cancer 83, 1084-1095 (2000).
22. Ö. Ostling and K. J. Johanson, Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123, 291-298 (1984).
23. V. A. Bohr, Gene specific DNA repair. Carcinogenesis 12, 1983-1992 (1991).
24. T Boulikas, The non-uniform repair of active and inactive chromatin domains. Int. J. Oncol. 8, 65-75 (1996).
25. N. F. Rajab, P. W. Hamilton, J. Diamond and V. J. McKelvey-Martin, An assessment of radiosensitivity in bladder cancer cell-lines. Irish J. Med. Sci. 169, 53 (1999).