Relationship between cellular radiosensitivity and DNA damage measured by comet assay in human normal, NBS and AT fibroblasts

International Journal of Radiation Biology

Volumn 77, Issue 9, 2001, Pages 929-938

  Brammer, I ^a   Zoller, M ^a   Dikomey, E ^a  

^a UNIVERSITY OF HAMBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL KINETICS; CHROMATIN STRUCTURE; COMET ASSAY; DNA DAMAGE; DNA STRAND BREAKAGE; FIBROBLAST; GEL ELECTROPHORESIS; HUMAN; HUMAN CELL; PRIORITY JOURNAL; RADIATION DOSE; RADIOSENSITIVITY;

ATAXIA TELANGIECTASIA; CELL LINE; COMET ASSAY; DNA DAMAGE; DNA REPAIR; FIBROBLASTS; HUMANS; RADIATION TOLERANCE;

EID: 0034831919     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/09553000110064222     Document Type: Article

References (47)

1. Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays
2. Relationship between in vitro chromosomal radiosensitivity of peripheral blood lymphocytes and the expression of normal tissue damage following radiotherapy for breast cancer
3. Optimization and standardization of the 'Comet Assay' for analyzing the repair of DNA damage in cells
4. In vitro radiosensitivity and normal tissue damage
5. Absence of ATM truncations in patients with severe acute radiation reactions
6. Relationship between cellular radiosensitivity and non-repaired double-strand breaks studied for different growth states, dose rates and plating conditions in a normal human fibroblast line
7. Relationship between DNA double-strand breaks, cell killing, and fibrosis studied in confluent skin fibroblasts derived from breast cancer patients
8. Correlation between cellular radiosensitivity and non-repaired double-strand breaks studied in nine mammalian cell lines
9. Identification of ataxia telangiectasia heterozygotes, a cancer-prone population, using the single-cell gel electrophoresis (comet) assay
10. Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters
11. The comet assay: A comprehensive review
12. Normal rejoining of DNA strand breaks in Ataxia Telangiectasia fibroblast lines after low X-ray exposure
13. Radiosensitivity of normal fibroblasts from breast cancer patients assessed by the micronucleus and colony assays
14. Abnormal radiosensitivity of lymphocytes from breast cancer patients with excessive normal tissue damage after radiotherapy: Chromosome aberrations after low doserate irradiation
15. Ku70/80 gene expression and DNA-dependent protein kinase (DNA-PK) activity do not correlate with double-strand break (dsb) repair capacity and cellular radiosensitivity in normal human fibroblasts
16. The comet moment as a measure of DNA damage in the comet assay
17. Lack of correlation between residual radiation-induced DNA damage, in keratinocytes assayed directly from skin, and late radiotherapy reactions in breast cancer patients
18. A correlation between residual DNA double-strand breaks and clonogenic measurements of radiosensitivity in fibroblasts from preradiotherapy cervix cancer patients
19. A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients
20. Radiation sensitivity correlates with changes in DNA supercoiling and nucleoid protein content in cells of three Chinese hamster cell lines
21. DNA supercoiling changes and nucleoid protein composition in a group of L5178Y cells of varying radiosensitivity
22. The single cell gel electrophoresis assay (comet assay): A European review
23. Comet assay studies of radiation-induced DNA damage and repair in various turnout cell lines
24. Radiation-induced micronucleus formation in human skin fibroblasts of patients showing severe and normal tissue damage after radiotherapy
25. Is chromosome in situ suppression (CISS) hybridization suited as a predictive test for intrinsic radiosensitvity in cancer patients?
26. Radiation-induced micronuclei in human fibroblasts in relation to clonogenic radiosensitivity
27. DNA organization affects cellular radiosensitivity and detection of initial DNA strand breaks
28. DNA damage and repair in individual cells: Applications of the comet assay in radiobiology
29. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the 'comet' assay
30. DNA double-strand breaks measured in individual cells subjected to gel electrophoresis
31. Factors influencing DNA migration from individual cells subjected to gel electrophoresis
32. Radiation-induced comet-formation in human skin fibroblasts from radiotherapy patients with different normal tissue reactions
33. Influence of ATM function on interactions between telomeres and nuclear matrix
34. Initial chromosome damage but not DNA damage is greater in ataxia telangiectasia cells
35. The contribution of DNA and chromosome repair deficiencies to the radiosensitivity of ataxia-telangiectasia
36. Increased initial levels of chromosome damage and heterogeneous chromosome repair in ataxia telangiectasia heterozygote cells
37. Chomosome end associations, telomeres and telomerase activity in ataxia telangiectasia cells
38. Comparison between pulsed-field gel electrophoresis and the comet assay as predictive assays for radiosensitivity in fibroblasts
39. Altered metaphase chromosome structure in xrs-5 cells is not related to its radiation sensitivity or defective DNA break rejoining
40. A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides
41. Differences in the DNA supercoiling response of irradiated cell lines from ataxia-telangiectasia versus unaffected individuals
42. New link in a web of human genes
43. Comparison between pulsed-field and constant-field gel electrophoresis for measurement of DNA double-strand breaks in irradiated Chinese hamster ovary cells
44. Cellular radiosensitivity and DNA damage in primary human fibroblasts
45. Nuclear scaffold organization in the X-ray sensitive Chinese hamster mutant cell line, xrs-5
46. The radiosensitivity of human flbroblast cell lines correlates with residual levels of DNA double-strand breaks