Radiosensitivity in breast cancer assessed by the Comet and micronucleus assays

British Journal of Cancer

Volumn 94, Issue 8, 2006, Pages 1194-1203

  Djuzenova, C S ^a   Muhl B ^a   Fehn, M ^a   Oppitz, U ^a   Muller B ^a   Flentje, M ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

DNA damage; DNA repair; Peripheral blood lymphocytes; Single cell gel electrophoresis

Indexed keywords

DNA FRAGMENT;

ADULT; AGED; ARTICLE; BLOOD SAMPLING; BREAST CANCER; CLINICAL ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; DOSE RESPONSE; FEMALE; GENOMIC INSTABILITY; HUMAN; HUMAN CELL; MICRONUCLEUS TEST; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; RADIATION INJURY; RADIATION RESPONSE; RADIOSENSITIVITY; SKIN MANIFESTATION; SMOKING; STATISTICAL ANALYSIS; X IRRADIATION;

ADOLESCENT; ADULT; AGED; BREAST NEOPLASMS; COMET ASSAY; DNA DAMAGE; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, RADIATION; FEMALE; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MICRONUCLEUS TESTS; MIDDLE AGED; PREDICTIVE VALUE OF TESTS; RADIATION TOLERANCE; RISK FACTORS; SENSITIVITY AND SPECIFICITY; SEVERITY OF ILLNESS INDEX; SKIN; X-RAYS;

EID: 33646126300     PISSN: 00070920     EISSN: 15321827     Source Type: Journal    
DOI: 10.1038/sj.bjc.6603005     Document Type: Article

References (45)

1. Alapetite C, Thirion P, de la Rochefordiere A, Cosset JM, Moustacchi E (1999) Analysis by alkaline comet assay of cancer patients with severe reactions to radiotherapy: defective rejoining of radioinduced DNA strand breaks in lymphocytes of breast cancer patients. Int J Cancer 83: 83-90
2. Baeyens A, Thierens H, Claes K, Poppe B, Messiaen L, De Ridder L, Vral A (2002) Chromosomal radiosensitivity in breast cancer patients with a known or putative genetic predisposition. Br J Cancer 87: 1379-1385, (doi:10.1038/sj.bjc.6600628)
3. Baria K, Warren SA, West CM, Scott D (2001) Chromosomal radiosensitivity as a marker of predisposition to common cancers? Br J Cancer 84: 892-896, (doi:10.1054/bjc.2000.1701)
4. Blasiak J, Arabski M, Krupa R, Wozniak K, Rykala J, Kolacinska A, Morawiec Z, Drzewoski J, Zadrovny M (2004) Basal, oxidative and alkylative DNA damage, DNA repair efficacy and mutagen sensitivity in breast cancer. Mutat Res 554: 139-148, (doi:10.1016/j.mrfmmm.2004.04.001)
5. Burrill W, Barber JB, Roberts SA, Bulman B, Scott D (2000) Heritability of chromosomal radiosensitivity in breast cancer patients: a pilot study with the lymphocyte micronucleus assay. Int J Radiat Biol 76: 1617-1619
6. Cao J, Liu Y, Sun H, Cheng G, Pang X, Zhou Z (2002) Chromosomal aberrations, DNA strand breaks and gene mutations in nasopharyngeal cancer patients undergoing radiation therapy. Mutat Res 504: 85-90, (doi:10.1016/S0027-5107(02)00082-9)
7. Carney JP (1999) Chromosomal breakage syndromes. Curr Opin Immunol 11: 443-447
8. Colleu-Durel S, Guitton N, Nourgalieva K, Legue F, Levêque J, Danic B, Chenal C (2004) Alkaline single-cell gel electrophoresis (Comet assay): a simple technique to show genomic instability in sporadic breast cancer. Eur J Cancer 40: 445-451, (doi:10.1016/j.ejca.2003.09.033)
9. Cox JD, Stetz J, Pajak TF (1995) Toxicity criteria of the radiation therapy oncology group (RTOG) and the European organization for research and treatment of cancer (EORTC). Int J Radiat Oncol Biol Phys 31: 1341-1346
10. Djuzenova CS, Rothfuss A, Oppitz U, Speit G, Schindler D, Hoehn H, Flentje M (2001) Response to X-irradiation of Fanconi anemia heterozygous cell assessed by the single-cell gel electrophoresis (Comet) assay. Lab Invest 81: 185-192
11. Djuzenova CS, Schindler D, Stopper H, Hoehn H, Flentje M, Oppitz U (1999) Identification of ataxia-telangiectasia heterozygotes, a cancer prone population, using the single-cell gel electrophoresis (Comet) assay. Lab Invest 79: 699-705
12. Fenech M (1993) The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutat Res 285: 35-44
13. Fenech M, Denham J, Francis W, Morley A (1990) Micronuclei in cytokinesis-blocked lymphocytes of cancer patients following fractionated partial-body radiotherapy. Int J Radiat Biol 57: 373-383
14. Fenech M, Morley AA (1985a) Measurement of micronuclei in lymphocytes. Mutat Res 147: 29-36
15. Fenech M, Morley AA (1985b) The effect of donor age on spontaneous and induced micronuclei. Mutat Res 148: 99-105
16. Garaj-Vrhovac V, Kopjar N (2003) The alkaline Comet assay as biomarker in assessment of DNA damage in medical personnel occupationally exposed to ionizing radiation. Mutagenesis 18: 265-271
17. Hadjidekova VB, Bulanova M, Bonassi S, Neri M (2003) Micronucleus frequency is increased in peripheral blood lymphocytes of nuclear power plant workers. Radiat Res 160: 684-690
18. Helzlsouer KJ, Harris EL, Parshad R, Fogel S, Bigbee WL, Sanford KK (1995) Familial clustering of breast cancer: possible interaction between DNA repair proficiency and radiation exposure in the development of breast cancer. Int J Cancer 64: 14-17
19. Hsu TC, Johnston DA, Cherry LM, Ramkissoon D, Schantz SP, Jessup JM, Winn RJ, Shirley L, Furlong C (1989) Sensitivity to genotoxic effects of bleomycin in humans: possible relationship to environmental carcinogenesis. Int J Cancer 43: 403-409
20. Hussien MM, McNulty H, Armstrong N, Johnston PG, Spence RAJ, Barnett Y (2005) Investigation of systemic folate status, impact of alcohol intake and levels of DNA damage in mononuclear cells of breast cancer patients. Br J Cancer 92: 1524-1530, (doi:10.1038/sj.bjc.6602530)
21. Lengauer C, Kinzler KW, Vogelstein B (1998) Genetic instabilities in human cancers. Nature 396: 643-649
22. Müller WU, Nüsse M, Miller BM, Slavotinek A, Viaggi S, Streffer C (2003) Micronuclei: a biological indicator of radiation damage. Mutat Res 366: 163-169
23. Müller-Vogt US, Maurer JEE, Grabenbauer GG, Distel LVR, Iro H, Steinhart H (2003) Altered DNA repair capacity in young patients suffering from multiple cancers. Int J Mol Med 11: 669-674
24. Norman A, Kagan AR, Chan SL (1988) The importance of genetics for the optimization of radiation therapy. A hypothesis. Am J Clin Oncol 11: 84-88
25. Olive PL, Banáth JP, Durand RE (1990) Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the 'Comet' assay. Radiat Res 122: 86-94
26. Parkin DM, Pisani P, Ferlay J (1999) Estimates of the worldwide incidence of 25 major cancers in 1990. Int J Cancer 80: 827-841
27. Parshad R, Price FM, Bohr VA, Cowans KH, Zujewski JA, Sanford KK (1996) Deficient DNA repair capacity, a predisposing factor in breast cancer. Br J Cancer 74: 1-5
28. Peto J, Collins N, Barfoot R, Seal S, Warren W, Rahman N, Easton DF, Evans C, Deacon J, Stratton MR (1999) Prevalence of BRCA1 and BRCA2 gene mutations in patients with early-onset breast cancer. J Natl Cancer Inst 91: 943-949
29. Rajaee-Behbahani N, Schmezer P, Risch A, Rittgen W, Kayser KW, Dienemann H, Schulz V, Drings P, Thiel S, Bartsch H (2001) Altered DNA repair capacity and bleomycin sensitivity as risk markers for non-small cell lung cancer. Int J Cancer (Pred Oncol) 95: 86-91
30. 2-phase lymphocytes identifies breast cancer patients with distinctive tumour characteristics. Br J Cancer 85: 1157-1161
31. Rigaud O, Guedeney G, Duranton I, Leroy A, Doloy MT, Magdelenat H (1990) Genotoxic effects of radiotherapy and chemotherapy on the circulation lymphocytes of breast cancer patients. Mutat Res 242: 25-35
32. Roberts SA, Spreadborough AR, Bulman B, Barber JBP, Evans DGR, Scott D (1999) Heritability of cellular radiosensitivity: a marker of low-penetrance predisposition genes in breast cancer. Am J Hum Genet 65: 784-789
33. Rothfuß A, Schütz P, Bochum S, Volm T, Eberhardt E, Kreienberg R, Vogel W, Speit G (2000) Induced micronucleus frequencies in peripheral lymphocytes as a screening test for carriers of a BRCA1 mutation in breast cancer families. Cancer Res 60: 390-394
34. Schabath MB, Spitz MR, Grossman HB, Zhang K, Dinney CP, Zheng P-J, Wu X (2003) Genetic instability in bladder cancer assessed by the Comet assay. J Natl Cancer Inst 95: 540-547
35. Scott D (2004) Chromosomal radiosensitivity and low penetrance predisposition to cancer. Cytogenet Genome Res 104: 365-370
36. Scott D, Barber JB, Spreadborough AR, Burrill W, Roberts SA (1999) Increased chromosomal radiosensitivity in breast cancer patients: a comparison of two assays. Int J Radiat Biol 75: 1-10
37. Scott D, Barber JB, Levine EL, Burrill W, Roberts SA (1998) Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition? Br J Cancer 77: 614-620
38. Singh NP, Danner DB, Tice RR, Brant L, Schneider EL (1990) DNA damage and repair with age in individual human lymphocytes. Mutat Res 237: 123-130
39. Singh NP, McCoy MT, Tice RR, Schneider E (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175: 184-191
40. Slonina D, Klimek M, Szpytma T, Gasinska A (2000) Comparison of the radiosensitivity of normal-tissue cells with normal-tissue reactions after radiotherapy. Int J Radiat Biol 76: 1255-1264
41. Speit G, Trenz K (2004) Chromosomal mutagen sensitivity associated with mutations in BRCA genes. Cytogenet Genome Res 104: 325-332
42. Stopper H, Hempel K, Reiners C, Vershenya S, Lorenz R, Vukicevic V, Heidland A, Grawe J (2005) Pilot study for comparison of reticulocyte-micronuclei with lymphocyte-micronuclei in human biomonitoring. Toxicol Lett 156: 351-360, (doi:10.1016/j.toxlet.2004.12.007)
43. Teare MD, Wallace SA, Harris M, Howell A, Birch JM (1994) Cancer experience in the relatives of an unselected series of breast cancer patients. Br J Cancer 70: 102-111
44. Thierens H, Vral A, Morthier R, Aousalah B, De Ridder L (2000) Cytogenetic monitoring of hospital workers occupation ally exposed to ionizing radiation using the micronucleus centromere assay. Mutagenesis 15: 245-249
45. Thompson LH, Schild D (2002) Recombinational DNA repair and human disease. Mutat Res 509: 49-78, (doi:10.1016/S0027-5107(02)00224-5)