UVA-induced apoptosis studied by the new apo/necro-Comet-assay which distinguishes viable, apoptotic and necrotic cells

Mutagenesis

Volumn 21, Issue 2, 2006, Pages 105-114

  Morley, N ^a   Rapp, A ^b   Dittmar, H ^b   Salter, L ^a   Gould, D ^a   Greulich, K O ^b   Curnow, A ^a  

^a ROYAL CORNWALL HOSPITAL   (United Kingdom)

^b FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CALCEIN; DNA FRAGMENT; ETHIDIUM; ETOPOSIDE; LIPOCORTIN 5;

APOPTOSIS; ARTICLE; CELL DEATH; CELL VIABILITY; COMET ASSAY; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DAMAGE; DNA REPAIR; GENE TRANSLOCATION; HEAT; HUMAN; HUMAN CELL; KINETICS; LABORATORY DIAGNOSIS; PRIORITY JOURNAL; RADIATION EXPOSURE; ULTRAVIOLET A RADIATION;

ANNEXIN A5; APOPTOSIS; CELLS, CULTURED; COMET ASSAY; DNA FRAGMENTATION; ETOPOSIDE; HUMANS; IN SITU NICK-END LABELING; KINETICS; NECROSIS; NUCLEIC ACID SYNTHESIS INHIBITORS; PESTICIDES; TIME FACTORS; TRYPAN BLUE; ULTRAVIOLET RAYS;

EID: 33645839303     PISSN: 02678357     EISSN: 14643804     Source Type: Journal    
DOI: 10.1093/mutage/gel004     Document Type: Article

References (32)

1. Cadet,J., Berger,M., Douki,T., Morin,B., Raoul,S., Ravanat,J.L. and Spinelli,S. (1997) Effects of UV and visible radiation on DNA-final base damage. Biol. Chem., 378, 1275-1286.
2. Urbach,F. (1997) Ultraviolet radiation and skin cancer of humans. J. Photochem. Photobiol. B Biol., 40, 3-7.
3. Young,A.R. (1999) The molecular and genetic effects of ultraviolet radiation exposure on skin cells. In Hawk,J.L.M. (ed.), Photodermatology. Oxford University Press, Oxford, UK, pp. 25-42.
4. De Gruijl,F.R. (2002) Photocarcinogenesis: UVA versus UVB Radiation. Skin Pharmacol. Appl. Skin Physiol., 15, 316-320.
5. Phillipson,R.P., Tobi,S.E., Morris,J.A. and McMillan,T.J. (2002) UV-A induces persistent genomic instability in human keratinocytes through an oxidative stress mechanism. Free Radic. Biol. Med., 32, 474-480.
6. Chaudiere,J. and Ferrari-Iliou,R. (1999) Intracellular antioxidants: from chemical to biochemical mechanisms. Food Chem. Toxicol., 37, 949-962.
7. Davies,K. (1996) Oxidative stress: the paradox of aerobic life. Biochem. Soc. Symp., 61, 1-31.
8. Shuler,M. and Green,D.R. (2001) Mechanisms of p53 dependent apoptosis. Biochem. Soc. Trans., 29, 684-688.
9. Singh,N.P., McCoy,M.T., Tice,R.R. and Schneider,E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res., 175, 184-191.
10. Singh,N.P. (2000) Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat. Res., 455, 111-127.
11. Vaux,D.L. and Strasser,A. (1996) The molecular biology of apoptosis. Proc. Natl Acad. Sci. USA, 93, 2239-2244.
12. Kokileva,L. (1994) Multi-step chromatin degradation in apoptosis. DNA breakdown in apoptosis. Int. Arch. Allergy Immunol., 105, 339-343.
13. Adrain,C. and Martin,S.J. (2001) The mitochondrial apoptosome: a killer unleashed by the cytochrome seas. Trends Biochem. Sci., 26, 390-397.
14. Heatwole,V.M. (1999) TUNEL assay for apoptotic cells. Methods Mol. Biol., 115, 141-148.
15. Bardeleben,R., Kaina,B. and Fritz,G. (2003) Ultraviolet light-induced apoptotic death is impaired by the HMG-CoA reductase inhibitor lovastatin. Biochem. Biophys. Res. Commun., 307, 401-407.
16. Dunkern,T.R., Fritz,G. and Kaina,B. (2001) Ultraviolet light-induced DNA damage triggers apoptosis in nucleotide excision repair-deficient cells via Bcl-2 decline and caspase-3/-8 activation. Oncogene, 20, 6026-6038.
17. Jiang,X. and Wang,X. (2004) Cytochrome c-mediated apoptosis. Annu. Rev. Biochem., 73, 87-106.
18. Martin,D.A. and Elkon,K.B. (2004) Mechanisms of apoptosis. Rheum. Dis. Clin. North Am., 30, 441-454, vii. Review.
19. Norbury,C.J. and Zhivotovsky,B. (2004) DNA damage-induced apoptosis. Oncogene, 23, 2797-2808.
20. Bacso,Z., Everson,R.B. and Eliason,J.F. (2000) The DNA of annexin V-binding apoptotic cells is highly fragmented. Cancer Res., 60, 4623-4628.
21. Boukamp,P., Petrussevska,R.T., Breitkreutz,D., Hornung,J., Markham,A. and Fusenig,N.E. (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol., 106, 761-771.
22. Olive,P.L., Wlodek,D., Durand,R.E. and Banath,J.P. (1992) Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res., 198, 259-267.
23. Rapp,A. and Greulich,K.O. (2004) After double stands break induction by UV-A homologous recombination and non homologous end joining cooperate at the same DSB if both systems are available. J. Cell Sci., 117, 4935-4945.
24. Choucroun,P., Gillet,D., Dorange,G., Sawicki,B. and Dewitte,J.D. (2001) Comet assay and early apoptosis. Mutat. Res., 478, 89-96.
25. Fairbairn,D.W., Walburger,D.K., Fairbairn,J.J. and O'Neill,K.L. (1996) Key morhologic changes and DNA strand breaks in human lymphoid cells: discriminating apoptosis from necrosis. Scanning, 18, 407-416.
26. Godard,T., Deslandes,E., Lebailly,R., Vigreux,C., Sichel,F., Poul,J.M. and Gauduchon,P. (1999) Early detection of staurosporine-induced apoptosis by comet and annexin V assays. Histochem. Cell Biol., 112, 155-161.
27. Gopalakrishna,P. and Khar,A. (1995) Comet assay to measure DNA-damage in apoptotic cells. J. Biochem. Biophys. Meth., 30, 69-73.
28. Krizilian,N., Wilkins,R.C., Reinhardt,P., Ferrarotto,C., McLean,J.R.N. and McNamee,J.P. (1999) Silver-stained Comet-assay for the detection of apoptosis. Biotechniques, 27, 926-930.
29. O'Callaghan,Y.C., Woods,J.A. and O'Brien,N.M. (2001) Limitations of the single-cell gel electrophoresis assay to monitor apoptosis in U937 and HepG2 cells exposed to 7 beta-hydroxycholesterol. Biochem. Pharmacol., 61, 1217-1226.
30. Olive,P.L., Frazer,G. and Banath,J.P. (1993) Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat. Res., 136, 130-136.
31. Rapp,A., Bock,C., Dittmar,H. and Greulich,K.O. (2000) UV-A breakage sensitivity of human chromosomes as measured by COMET- FISH depends on gene density and not on the chromosome size. J. Photochem. Photobiol. B, 56, 109-117.
32. Meintieres,S., Nesslany,F., Pallardy,M. and Marzin,D. (2003) Detection of ghost cells in the standard alkaline comet assay is not a good measure of apoptosis. Environ. Mol. Mutagen., 41, 260-269.