Detection of hypoxic cells in a C3H mouse mammary carcinoma using the comet assay

British Journal of Cancer

Volumn 76, Issue 6, 1997, Pages 694-699

  Olive, P L ^a   Horsman, M R ^b   Grau, C ^b   Overgaard, J ^b  

^a BRITISH COLUMBIA CANCER RESEARCH CENTRE   (Canada)

^b DANISH CANCER SOCIETY RESEARCH CENTER   (Denmark)

Author keywords

DNA strand break; Radiosensitivity; Tumour control; Tumour hypoxia

Indexed keywords

CARBOGEN; CARBON MONOXIDE; OXYGEN;

ANIMAL EXPERIMENT; ARTICLE; BREAST CARCINOMA; DNA STRAND BREAKAGE; EXPERIMENTAL NEOPLASM; HYPOXIC CELL; MALE; MOUSE; NONHUMAN; OXYGEN BREATHING; PRIORITY JOURNAL; RADIOBIOLOGY; RADIOSENSITIVITY; SQUAMOUS CELL CARCINOMA;

EID: 0030818472     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/bjc.1997.448     Document Type: Article

References (41)

1. Bentzen SM and Grau C (1991) Direct estimation of the fraction of hypoxic cells from tumour-control data obtained under aerobic and clamped conditions. Inter J Radiat Biol 59: 1435-1440
2. Biade S, Yeh KA, Milito SJ, Brown DQ, Lanciano RM and Chapman JD (1995) Electrode measurements of oxygen tensions in rat prostate carcinomas and comparison with other assays. In Tumour Oxygenation, Vaupel P, Kelleher DK and Gunderoth M (eds), pp. 83-94. Gustav Fischer: Stuttgart
3. Bush RS, Jenkin RD, Allt WE, Beale A, Bean H, Demko AJ and Pringle JF (1978) Definitive evidence for hypoxic cells influencing cure in cancer therapy. Br J Cancer 37 (Suppl): 302-306
4. Cater DB, Silver EA and Wilson BM (1959) Apparatus and technique for the quantitative measurement of oxygen tension in living tissues. Proc Royal Soc B 151: 256-276
5. Chapman JD, Dugle DL, Reuvers AP, Meeker BE, and Borsa J (1974) Studies on the radiosensitizing effect of oxygen in Chinese hamster cells. Inter J Radiat Biol 26: 383-389
6. Cline JM, Thrall DE, Rosner GL, and Raleigh JA (1994) Distribution of the hypoxia marker CCI-103F in canine tumours. Inter J Radiat Oncol Biol Phys 28: 921-933
7. Dorie MJ and Brown JM (1995) Potentiation of the anticancer effect of cisplatin by the hypoxic cytotoxin tirapazamine. In Tumour Oxygenation. Vaupel P, Kelleher DK and Gunderoth M, (eds), pp. 125-135. Gustav Fischer: Stuttgart
8. Fenton BM, Kiani MF and Siemann DW (1995) Should direct measurements of tumour oxygenation relate to the radiobiological hypoxic fraction of a tumour? Inter J Radiat Oncol Biol Phys 33: 365-373
9. Franko AJ, Koch CJ, Garrecht BM, Sharplin J and Hughes D (1987) Oxygen concentration dependence of binding of misonidazole to rodent and human tumours in vitro. Cancer Res 47: 5367-5376
10. Gatenby RA, Kessler HB, Rosenblum JS, Coia LR, Moldofsky PJ, Hartz WH and Broder GJ (1988) Oxygen distribution in squamous cell carcinoma metastases and its relationship to outcome of radiation therapy. Inter J Radiat Oncol Biol Phys 14: 831-838.
11. Gerweck LE, Koutcher J and Zaidi ST (1995) Energy status parameters, hypoxia fraction and radiocurability across tumour types. Acta Oncol 34: 335-338
12. Gonzalez DG (1991) Hypoxia and local tumour control. Part I. Radiother Oncol Suppl 20: 5-7
13. Grau C, Nordsmark M, Khalil AA, Horsman MR and Overgaard J (1994) Effect of carbon monoxide breathing on hypoxia and radiation response in the SCCVII tumor in vivo. Inter J Radiat Oncol Biol Phys 29: 449-454
14. Gray LH, Conger AD, Ebert M, Hornsey S and Scott OCA (1953) The concentration of oxygen dissolved in tissues at the time of irradiation as a factor in radiotherapy. Br J Radiol 26: 638-648
15. 2 predicts survival in advanced cancer of uterine cervix. Radiother Oncol 26: 45-50
16. Horsman MR, Khalil AA, Nordsmark M, Grau C and Overgaard J (1993) Relationship between radiobiological hypoxia and direct estimates of tumour oxygenation in a mouse tumour model. Radiother Oncol 28: 69-71
17. Horsman MR, Khalil AA, Siemann DW, Grau C, Hill SA, Lynch EM, Chaplin DJ and Overgaard J (1994) Relationship between radiobiological hypoxia in tumours and electrode measurements of tumour oxygenation. Inter J Radiat Oncol Biol Phys 29: 439-442
18. Horsman MR, Khalil AA, Nordsmark M, Siemann DW, Hill SA, Lynch M, Chaplin DJ, Stern S, Thomas CD, Guichard M, Grau C and Overgaard J (1995) The use of oxygen electrodes to predict radiobiological hypoxia in tumours. In Tumour Oxygenation, Vaupel P, Kelleher DK and Gunderoth M (eds), pp 49-57. Gustav Fischer: Stuttgart
19. Hu Q, Kavanagh M, Newcombe D and Hill RP (1995) Detection of hypoxic fractions in murine tumours by comet assay: Comparison with other techniques. Radiat Res 144: 266-275
20. 3H-misonidazole binding, and paired survival assay. Radiat Res 145: 491-500
21. Khalil AA, Horsman MR and Overgaard J (1995) The importance of determining necrotic fraction when studying the effect of tumour volume on tissue oxygenation. Acta Oncologica 34: 297-300
22. Kolstad P (1995) Intercapillary distance, oxygen tension and local recurrence in cervix cancer. Scand J Clin Lab Invest 106: 145-157
23. Martin LM, Thomas CD and Guichard M (1994) Nicotinamide and carbogen: relationship between pO2 and radiosensitivity in three tumour lines. Inter J Radiat Biol 65: 379-386
24. Moulder JE and Rockwell S (1984) Hypoxic fractions of solid tumours: experimental techniques, methods of analysis and a survey of existing data. Inter J Radiat Oncol Biol Phys 10: 695-712
25. Nordsmark M, Bentzen SM and Overgaard J (1994) Measurement of human tumour oxygenation status by a polarographic needle electrode. An analysis of inter-and intratumour heterogeneity. Acta Oncol 33: 383-389
26. Nordsmark M, Grau C, Horsman MR, Jorgensen HS and Overgaard J (1995) Relationship between tumour oxygenation, bioenergetic status and radiobiological hypoxia in an experimental model. Acta Oncol 34: 329-334
27. Nordsmark M, Overgaard M and Overgaard J (1996) Pretreatment oxygenation status predicts radiation response in advanced squamous cell carcinoma of the head and neck. Radiother Oncol 41: 31-39
28. Olive PL (1994) Radiation-induced reoxygenation in the SCCVII murine tumour: evidence for a decrease in oxygen consumption and an increase in tumour perfusion. Radiother Oncol 32: 37-46
29. Olive PL and Durand RE (1992) Detecting hypoxic cells in a murine tumour using the comet assay. J Natl Cancer Inst 85: 707-711
30. Olive PL, Banath JP and Durand RE (1990) Heterogeneity in radiation-induced DNA damage and repair in tumour and normal cells measured using the 'comet' assay. Radiat Res 122: 69-72
31. Olive PL, Durand RE, Le Riche J, Olivotto I and Jackson SM (1993) Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers. Cancer Res 53: 733-736
32. Olive PL, Vikse, CM and Durand RE (1994) Hypoxic fractions measured in murine tumours and normal tissues using the comet assay. Inter J Radiat Oncol Biol Phys 29: 487-491
33. Olive PL, Trotter T, Banath JP, Jackson SM and Le Riche J (1996) Heterogeneity in human tumour hypoxic fraction using the comet assay. Br J Cancer 74: S191-S196
34. Overgaard J (1980) Simultaneous and sequential hyperthermia and radiation treatment of an experimental tumour and its surrounding normal tissue in vivo. Inter J Radiat Oncol Biol Phys 6: 1507-1517
35. Overgaard J (1994) Clinical evaluation of nitroimidazoles as modifiers of hypoxia in solid tumours. Oncol Res 6: 509-518
36. 31P-NMR magnetic resonance spectroscopy studies of tumour energy metabolism and its relationship to intracapillary oxyhemoglobin saturation status and tumour hypoxia. Cancer Res 48: 5440-5446
37. Stone HB, Brown JM, Phillips TM and Sutherland RM (1993) Oxygen in human tumours: correlations between methods of measurement and response to therapy. Radiat Res 136: 422-434
38. Thomas CD and Guichard M (1996) Influence of melanin on pO2 measurement in vitro and in vivo. Inter J Radiat Biol 69: 205-211
39. 2 tension measurements. Cancer Res 51: 3316-3322
40. Zhang H and Wheeler KT (1994) Radiation-induced DNA damage in tumours and normal tissues. II. Influence of dose, residual DNA damage and physiological factors in oxygenated cells. Radiat Res 140: 321-326
41. Zhang H, Koch CJ, Wallen CA and Wheeler KT (1995) Radiation-induced DNA damage in tumours and normal tissues. III. Oxygen dependence of the formation of strand breaks and DNA-protein crosslinks. Radiat Res 142: 163-168