Detailed understanding of enhanced specific antibody productivity in NS0 myeloma cells

Biotechnology and Bioengineering

Volumn 102, Issue 1, 2009, Pages 188-199

  Khoo, Soo Hean Gary ^a,b   Al Rubeai, Mohamed ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Cell culture; Cell cycle; Monoclonal antibody; mRNA; NS0 myeloma; P21

Indexed keywords

CELL CYCLE; MONOCLONAL ANTIBODY; MRNA; NS0 MYELOMA; P21;

BIOMASS; CELL CULTURE; MODULATION; MONOCLONAL ANTIBODIES; PRODUCTIVITY; RENEWABLE ENERGY RESOURCES; SURFACE PLASMON RESONANCE;

ANTIBODIES;

CELL PROTEIN; MESSENGER RNA; POLYPEPTIDE; PROTEIN P21; ANTIBODY;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOMASS; CELL CULTURE; CELL GROWTH; CELL PROLIFERATION; CELL VOLUME; CONTROLLED STUDY; FLOW CYTOMETRY; HEAVY CHAIN; LIGHT CHAIN; MYELOMA CELL; NONHUMAN; PROTEIN EXPRESSION; RNA STABILITY; RNA TRANSCRIPTION; TRANSCRIPTION REGULATION; ANIMAL; BIOSYNTHESIS; CELL SIZE; METABOLISM; MOUSE; TUMOR CELL LINE;

ANIMALS; ANTIBODIES; BIOMASS; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SIZE; MICE; RNA STABILITY; RNA, MESSENGER;

EID: 58149214617     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22041     Document Type: Article

References (76)

1. CIPI in adaptation of CHO cells to suspension and protein-free culture.
2. J Biotechnol 130:282-290. Balcarcel RR, Stephanopoulos G. 2001. Rapamycin reduces hybridoma cell death and enhances monoclonal antibody production. Biotechnol Bioeng 76:1-10.
3. Barnabé N, Butler M. 1994. Effect of temperature on nucleotide pools and monoclonal antibody productivity in a mouse hybridoma. Biotechnol Bioeng 44:1235-1245.
4. Barnes LM, Bentley CM, Dickson AJ. 2001. Characterisation of the stability of recombinant protein production in the GS-NS0 expression system. Biotechnol Bioeng 73:261-270.
5. Barnes LM, Bentley CM, Dickson AJ. 2004. Molecular definition of predictive indicators of stable protein expression in recombinant NS0 myeloma cells. Biotechnol Bioeng 85:115-121.
6. Barnes LM, Moy N, Dickson AJ. 2006. Phenotypic variation during cloning proceduce: Analysis of the growth behaviour of clonal cell lines. Biotechnol Bioeng 94:530-537.
7. Barnes LM, Bentley CM, Moy N, Dickson AJ. 2007. Molecular analysis of successful cell line selection in transfected GS-NS0 myeloma cells. Biotechnol Bioeng 96:337-348.
8. Bebbington CR, Renner G, Thomson S, King D, Abrams D, Yarranton GT. 1992. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Bio/Technology 10:169-175.
9. Bergman LW, Harris E, Kuehl WM. 1981. Glycosylation causes an apparent block in translation of immunoglobin heavy-chain. J Biol Chem 256: 701-706.
10. CIPI- arrested CHO cells. Biotechnol Bioeng 85:741-749.
11. Bibila T, Flickinger MC. 1991. A structured model for monoclonal antibody synthesis in exponentially growing and stationary phase hybridoma cells. Biotechnol Bioeng 37:210-226.
12. Borth N, Heider R, Assadian A, Katinger H. 1992. Growth and production kinetics of human x mouse and mouse hybridoma cells at reduced temperature and serum content. J Biotechnol 25:319-331.
13. Borth N, Strutzenberger K, Kunert R, Steinfeller W, Katinger H. 1999. Analysis of changes during subclone development and aging of human antibody-producing heterohybridoma cells by Northern blot and flow cytometry. J Biotechnol 67:57-66.
14. Bruce SR, Dingle RWC, Peterson ML. 2003. B-cell and plasma-cell splicing differences: A potential role in regulated immunoglobulin RNA processing. RNA 9:1264-1273.
15. Burrows P, LeJeune M, Kearney JF. 1979. Evidence that murine pre-B cells synthesis μ heavy chains but no light chains. Nature 280:838-840.
16. Calame KL. 2001. Plasma cells: Finding new light at the end of B cell development. Nature Immunol 2:1103-1108.
17. Carroll S. 2005. The selection and monitoring of high producing recombi- nant animal cell lines using flow cytometry. PhD thesis, Unversity of Birmingham.
18. Carvalhal AV, Coroadinha AS, Alves PM, Moreira JL, Hauser H, Carrondo JT. 2002. Metabolic changes during cell growth inhibition by the IRF-1 system. Enzyme Microb Technol 30:95-109.
19. Collins ML, Zayati C, Detmar JJ, Daly B, Kolberg JA, Cha TA, Irvine BD, Tucker J, Urdea MS. 1995. Preparation and characterization of RNA standards for use in quantitative branched DNA hybridization assays. Anal Biochem 226:120-129.
20. Dawson RMC, Elliott DC, Elliott WH, Jones KM. 1986. Data for Biochemical Research. Oxford: Oxford University Press, pp. 256-257.
21. Dinnis DM, James DC. 2005. Engineering mammalian cell factories for improved recombinant monoclonal antibody production: Lessons from Nature? Biotechnol Bioeng 91:180-189.
22. Dinnis DM, Stansfield SH, Schlatter S, Smales CM, Alete D, Birch JR, Racher AJ, Marshall CT, Nielsen LK, James DC. 2006. Functional proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibodyproduction rate. Biotechnol Bioeng 94:830-841.
23. Dorai H, Csirke B, Scallon B, Ganguly S. 2006. Correlation of heavy and light chain mRNA copy numbers to antibody productivity in mouse myeloma production cell lines. Hybridoma 25:1-9.
24. Dutton RL, Scharer JM, Moo-Young M. 1998. Descriptive parameter evaluation in mammalian cell culture. Cytotechnology 26:139-152.
25. Eckmann L, Huang GT-J, Smith JR, Morzycka-Wroblewska E, Kagnoff MF. 1994. Increased transcription and coordinate stabilization of mRNAs for secreted immunoglobulin α heavy chain and κ light chain following stimulation of immunoglobulin A expressing B cells. J Biol Chem 269: 33102-33108.
26. Flickinger MC, Goebel NK, Bibila T, Boyce-Jacino S. 1992. Evidence for posttranscriptional stimulation of monoclonal antibody secretion by L-glutamine during slow hybridoma growth. J Biotechnol 22: 201-226.
27. Fox SR, Tan HK, Tan MC, Wong SCNC, Yap MGS, Wang DIC. 2005. A detailed understanding ofthe enhanced hypothermic productivityof interferon-g by Chinese hamster ovary cells. Biotechnol Appl Biochem 41:255-264.
28. Furukawa K, Ohsuye K. 1998. Effect of culture temperature on a recombi- nant CHO cell line producing a C terminal α-amidating enzyme. Cytotechnology 26:153-164.
29. Furukawa K, Ohsuye K. 1999. Enhancement of productivity of recombinant a-amidating enzyme by low temperature culture. Cytotechnology 31:85-94.
30. Fussenegger M, Bailey JE. 1998. molecular regulation of cell-cycle progression and apoptosis in mammalian cells: Implications for biotechnology. Biotechnol Prog 14:807-833.
31. Fussengger M, Betenbaugh MJ. 2002. Metabolic engineering II. Eukaryotic systems. Biotechnol Bioeng 79:509-531.
32. Fussenegger M, Mazur X, Bailey JE. 1997. A novel cytostatic process enhances the productivity of Chinese Hamster Ovary cells. Biotechnol Bioeng 55:927-939.
33. Fussengger M, Schlatter S, Dätwyler D, Mazur X, Bailey JE. 1998. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese Hamster Ovary cells. Nature Biotechnol 16:468472.
34. Gódia F, Cairó JJ. 2002. Metabolic engineering of animal cells. Bioprocess Biosyst Eng 24:289-298.
35. Gonzalez R, Andrews BA, Asenjo JA. 2002. Kinetic model of BiP- and PDI- mediated protein folding and assembly. J Theor Biol 214:529-537.
36. Goodman LS, Gilman A. 1990. The pharmacological basis of therapeutics. Oxford: Pergamon Press, pp. 1240-1241.
37. Harrold S, Genovese C, Kobrin B, Morrison SL, Milcarek C. 1991. A comparison of apparent mRNA half-life using kinetic labeling techniques vs decay following administration of transcriptional inhibitors. Anal Biochem 198:19-29.
38. Ibarra N, Watanbe S, Bi J-X, Shuttleworth J, Al-Rubeai M. 2003. Modulation of cell cycle for enhancement of antibody productivity in perfusion culture of NS0 cells. Biotechnol Prog 19:224-228.
39. Kaufmann H, Mazur X, Marone R, Carson C, Fussenegger M. 2001. Comparative analysis of two controlled proliferation strategies regarding product quality: Influence on tetracycline-regulated gene expression, and productivity. Biotechnol Bioeng 72:592-602.
40. Kim NS, Kim SJ, Lee GM. 1998. Clonal variability within Dihydrofolate Reductase-mediated gene amplification Chinese hamster ovary cells: Stability in the absense of selective pressure. Biotechnol Bioeng 60:679688.
41. Kirchhoff S, Koromilas A, Schaper F, Grashoff M, Sonenberg N, Hauser H. 1995. IRF-1 induced cell growth inhibition and interferon induction requires the activity of the protein kinase PKR. Oncogene 11:439-445.
42. Kirchhoff S, Kröger A, Cruz H, Tümmler M, Schaper F, Hauser H. 1996. Regulation of cell growth by IRF-1 in BHK-21 cells. Cytotechnology 22:147-156.
43. Kuystermans D, Krampe B, Swiderek H, Al-Rubeai M. 2007. Using cell engineering and omic tools for the improvement of cell culture processes. Cytotechnology 53:3-22.
44. Lattenmayer C, Loeschel M, Schriebl K, Steinfellner W, Sterovsky T, Trummer E, Vorauer-Uhl K, Müller D, Katinger H, Kunert R. 2007. Protein-free transfection of CHO host cells with an IgG-Fusion protein: Selection and characterization of stable high producers and comparison to conventionally transfected clones. Biotechnol Bioeng 96:1118-1126.
45. Lee MS, Lee GM. 2000. Hyperosmotic pressure enhances immunoglobulin transcription rates and secretion rates of KR12H-2 transfectoma. Biotechnol Bioeng 68:260-268.
46. Lee MS, Lee GM. 2001. Effect of hypoosmotic pressure on cell growth and antibody production in recombinant Chinese Hamster ovary cell culture. Cytotechnology 36:61-69.
47. Lee GM, Park SY. 1995. Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress is cell line-specific. Biotechnol lett 17:145-150.
48. Leitzgen K, Knittler MR, Hass IG. 1997. Assembly of immunoglobulin light chains as a prerequisite for secretion. A model for oligomerization- dependent subunit folding. J Biol Chem 272:3117-3123.
49. Leno M, Merten OW, Hache J. 1992a. Kinetic analysis of hybridoma growth and monoclonal antibody production in semicontinuous culture. Biotechnol Bioeng 39:596-606.
50. Leno M, Merten OW, Hache J. 1992b. Kinetic studies of cellular metabolic activity, specific IgG production rate, IgG mRNA stability and accumulation during hybridoma batch culture. Enzyme Microb Technol 14:135-140.
51. Maki R, Kearney J, Paige C, Tonegawa S. 1980. Immunoglobulin gene arrangement in immature B cells. Science 209:1366-1369.
52. McNeely KM, Sun Z, Sharfstein ST. 2005. Techniques for dual staining of DNA and intracellular immunoglobulins in murine hybridoma cells: Applications to cell-cycle analysis of hyperosmotic cultures. Cytotech- nology 48:15-26.
53. KIPI-mediated controlled proliferation technology increases constitutive sICAM production in CHO-DUKX adapted for growth in suspension and serum- free media. Biotechnol Bioeng 79:619-627.
54. Milcarek C, Suda-hartman M, Croll SC. 1996. Changes in abundance pf IgG 2a mRNA in the nucleus and cytoplasm of a murine B-lymphoma before and after fusion to a myeloma cell. Mol Immunol 33:691-701.
55. Mullis KB, Faloona F. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 155:335-350.
56. Oguchi S, Saito H, Tsukahara M, Tsumura H. 2006. pH condition in temperature shift cultivation enhances cell longevity and specific hMab productivity in CHO culture. Cytotechnology 52:199-207.
57. Oh S, Chua F, Al-Rubeai M. 1996. Flow cytometric studies of osmotically stressed and sodium butyrate-treated hybridoma cells. In: Al-Rubeai M, Emery AN, editors. Flow cytometry applications in cell culture. New York: Marcel Dekker Inc., pp. 101-119.
58. Peterson ML, Bryman MB, Peiter M, Cowan C. 1994. Exon size affects competition between splicing and cleavage-polyadenylation in the Immunoglobulin μ gene. Mol Cell Biol 14:77-86.
59. Proudfoot NJ, Furger A, Dye MJ. 2002. Integrating mRNA processing with transcription. Cell 108:501-512.
60. Qu G-Z, Ehrlich M. 1999. Demethylation and expression of methylated plasmid DNA stably transfected into HeLa cells. Nucleic Acids Res 27:2332-2338.
61. Razin A, Cedar H. 1991. DNA methylation and gene expression. Microbiol Rev 55:451-458.
62. Ryu JS, Lee MS, Lee GM. 2001. Effects ofclone gene dosage on the response of recombinant CHO cells to hyperosmotic pressure in regard to cell growth and antibody production. Biotechnol Prog 17:993-999.
63. Sauer PW, Burky JE, Wesson MC, Sternard HD, Qu L. 2000. A high-yielding, generic fed-batch cell culture process for production of recombinant antibodies. Biotechnol Bioeng 67:585-597.
64. Schlatter S, Stansfield SH, Dinnis DM, Racher AJ, Birch JR, James DC. 2005. On the optimal ratio of heavy chain to light chain genes for efficient recombinant antibody production by CHO cells. Biotechnol Prog 21:122-133.
65. Shapiro-Shelef M, Calame KL. 2004. Plasma cell differentiation and multiple myeloma. Curr Opin Immunol 16:226-234.
66. Smales CM, Dinnis DM, Stansfield SH, Alete D, Sage EA, Birch JR, Racher AJ, Marshall CT, James DC. 2004. Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnol Bioeng 88:474488.
67. Sonenshein GE, Siekevitz M, Siebert GR, Gefter ML. 1978. Control of immunoglobulin sereton in the murine plasmacytoma line MOPC 315. J Exp Med 148:301-312.
68. Stansfield SH, Allen EE, Dinnis DM, Racher AJ, Birch JR, James DC. 2007. Dynamic analysis of GS-NS0 cells producing a recombinant antibody during fed-batch culture. Biotechnol Bioeng 97:410-424.
69. Stetefeld J, Ruegg MA. 2005. Structural and functional diversity generated by alternative mRNA splicing. Trends Biochem Sci 30:515-521.
70. Strutzenberger K, Borth N, Kunert R, Steinfeller W, Katinger H. 1999. Changes during subclone development and aging of human antibody-producing recombinant CHO cells. J Biotechnol 69:215226.
71. Sun Z, Zhou R, Liang S, McNeeley KM, Sharfstein ST. 2004. Hyperosmotic stress in murine hybridoma cells: Effects on antibody transcription, translation, posttranslational processing, and the cell cycle. Biotechnol Prog 20:576-589.
72. Underhill MF, Smales CM. 2007. The cold-shock response in mammalian cells: Investigating the HeLa cold-shock proteome. Cytotechnology 53:47-53.
73. CIPI. Biotechnol Bioeng 77:1-7.
74. Yoo EM, Chintalacharuvu KR, Penichet ML, Morrison SL. 2002. Myeloma expression systems. J Immunol Methods 261:1-20.
75. Yoon SK, Song JY, Lee GM. 2003. Effect of low temperature on specific productivity, transcription level, and heterogeneity of Erythropoeitin in Chinese Hamster Ovary Cells. Biotechnol Bioeng 82:289-298.
76. Yoon SK, Hong JK, Choo SH, Song JY, Park HW, Lee GM. 2006. Adaptation of Chinese hamster ovary cells to low culture temperature: Cell growth and recombinant protein production. J Biotechnol 122: 463-472.