P27Kip1-Mediated controlled proliferation technology increases constitutive sICAM production in CHO-DUKX adapted for growth in suspension and serum-free media

Biotechnology and Bioengineering

Volumn 79, Issue 6, 2002, Pages 619-627

  Meents, Heiko ^a,b   Enenkel, Barbara ^b   Werner, Rolf G ^b   Fussenegger, Martin ^a  

^a ETH ZURICH   (Switzerland)

^b BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

Author keywords

Cell cycle; Controlled proliferation; Cyclin dependent kinase inhibitor; Gene switch; P27; Serum free; SICAM

Indexed keywords

CELLS; DISEASES; DRUG PRODUCTS; ENZYMES;

PROLIFERATION TECHNOLOGY; SERUM-FREE MEDIA;

BIOTECHNOLOGY;

CYCLIN DEPENDENT KINASE INHIBITOR; INTERCELLULAR ADHESION MOLECULE 1; PROTEIN P27; CELL CYCLE PROTEIN; CYCLIN DEPENDENT KINASE INHIBITOR 1B; DIHYDROFOLATE REDUCTASE; DRUG DERIVATIVE; ECDYSTERONE; ENZYME INHIBITOR; MURISTERONE A; TUMOR SUPPRESSOR PROTEIN;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL LINE; CHO CELL; DRUG SYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC ENGINEERING; NONHUMAN; PROTEIN EXPRESSION; WESTERN BLOTTING; ANIMAL; BIOSYNTHESIS; CELL DIVISION; COMPARATIVE STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; DOSE RESPONSE; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; HAMSTER; METHODOLOGY; PHYSIOLOGY; PROTEIN ENGINEERING; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SUSPENSION;

CRICETINAE; CRICETULUS GRISEUS;

ANIMALS; CELL CULTURE TECHNIQUES; CELL CYCLE PROTEINS; CELL DIVISION; CELL LINE; CHO CELLS; CRICETINAE; CULTURE MEDIA, SERUM-FREE; CYCLIN-DEPENDENT KINASE INHIBITOR P27; DOSE-RESPONSE RELATIONSHIP, DRUG; ECDYSTERONE; ENZYME INHIBITORS; GENE EXPRESSION REGULATION; INTERCELLULAR ADHESION MOLECULE-1; PROTEIN ENGINEERING; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SUSPENSIONS; TETRAHYDROFOLATE DEHYDROGENASE; TUMOR SUPPRESSOR PROTEINS;

EID: 0037144084     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10322     Document Type: Article

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