Changes in abundance of IgG 2a mRNA in the nucleus and cytoplasm of a murine B-lymphoma before and after fusion to a myeloma cell

Molecular Immunology

Volumn 33, Issue 7-8, 1996, Pages 691-701

  Milcarek, Christine ^a   Suda Hartman, Makiko ^a   Croll, Susan C ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Ig mRNA; mRNA; polyadenylation; RNA transport

Indexed keywords

IMMUNOGLOBULIN G2A; MESSENGER RNA; SECRETORY IMMUNOGLOBULIN;

ANIMAL CELL; ARTICLE; B CELL LYMPHOMA; CELL FUSION; CELL NUCLEUS; CYTOPLASM; HYBRIDOMA; MOUSE; MYELOMA CELL; NONHUMAN; PRIORITY JOURNAL; RNA TRANSCRIPTION;

ANIMALIA; MURINAE;

EID: 0030151995     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/0161-5890(96)00009-0     Document Type: Article

References (39)

1. Belgrader P., Cheng J., Zhou X., Stephenson L. S. and Maquat L. E. (1994) Mammalian nonsense codons can be cis effectors of nuclear mRNA half-life. Molec. cell. Biol. 14, 8219-8228.
2. Berberich I. and Schimpl A. (1990) Regulation of Ig gene expression in normal lymphocytes. I. The half-life of secreted μ chain mRNA differs from that of membrane μ chain mRNA in resting and activated B cells. Eur. J. Immun. 20, 445-448.
3. Cox A. and Emtage J. S. (1989) A 6-fold difference in the half-life of immunoglobulin μ heavy chain mRNA in cell lines representing two stages of B cell differentiation. Nucl. Acids Res. 17, 10439-10454.
4. Eckhardt L. A. (1992) Immunoglobulin gene expression only in the right cells at the right time. FASEB J. 6, 2553-2560.
5. Eckmann L., Huang G.T.-J., Smith J. R., Morzycka-Wroblewski E. and Kagnoff M. F. (1994) Increased transcription and coordinate stabilization of mRNAs for secreted immunoglobulin a heavy chain and κ light chain following stimulation of immunoglobulin A expressing B cells. J. biol. Chem. 52, 33102-33108.
6. Edwalds-Gilbert G., Prescott J. and Falck-Pederson E. (1993) 3′ RNA processing efficiency plays a primary role in generating termination-competent RNA polymerase II elongation complexes. Molec. cell. Biol. 13, 3472-3480.
7. Flaspohler J. A., Boczkowski D., Hall B. L. and Milcarek C. (1995) The 3′ untranslated region of membrane exon 2 from the gamma 2a immunoglobulin gene contributes to efficient transcription termination. J. biol. Chem. 270, 11903-11911.
8. Flaspohler J. A. and Milcarek C. (1990) Myelomas and lymphomas expressing the immunoglobulin gamma 2a heavy chain gene have similar transcription termination regions. J. Immun. 144, 2802-2810.
9. Flaspohler J. A. and Milcarek C. (1992) Use of in vitro transcribed RNAs as immobilized targets during RNA:RNA hybridization. Biotechniques 13, 68-72.
10. Friedman J. and Weissman I. (1991) Two cytoplasmic candidates for immunophilin action are revealed by affinity for a new cyclophilin: One in the presence and one in the absence o fCsA. Cell 66, 799-806.
11. Genovese C. and Milcarek C. (1990) Increased half-life of μ immunoglobulin mRNA during mouse B cell development increases its abundancy. Molec. Immun. 27, 733-743
12. Greenberg A., Ber R., Kra-Oz Z. and Laskov R. (1987) Extinction of expression of Ig genes in myeloma x fibroblast somatic cell hybrids. Molec. cell. Biol. 7, 936-939
13. Harlow E. and Eane D. (1988) In Antibodies, a Laboratory Manual. Cold Spring Harbor Laboratory, NY.
14. Harrold S., Genovese C., Kobrin B., Morrison S. L. and Milcarek C. (1991) A comparison of apparent mRNA half-life using kinetic labelling techniques versus decay following administration of transcriptional inhibitors. Analyt. Biochem. 198, 19-29.
15. Hirashima S., Hirai H., Nakanishi Y. and Natori S. (1988) Molecular cloning and characterization of cDNA for eucaryotic transcription factor SII. J. biol. Chem. 263, 3858-3863.
16. Hook A. G. and Kellems R. E. (1988) Localization and sequence analysis of poly(A) sites generating multiple dihyrofolate reductase mRNAs. J. biol. Chem. 263, 2337-2343.
17. Jack H.-M., Berg J. and Wabl M. (1989) Translation affects immunoglobulin mRNA stability. Eur. J. Immun. 19, 843-847.
18. Jack H.-M. and Wabl M. (1988) Immunoglobulin mRNA stability varies during B lymphocyte differentiation. EMBO J. 7, 1041-1046.
19. Kelley D. E. and Perry R. P. (1986) Transcriptional and post-transcriptional control of immunoglobulin mRNA production during B lymphocyte development. Nucl. Acids Res. 14, 5431-5447.
20. Kim K. J., Kanellopoulos-Langevin C., Merwin R., Sachs D. and Asofsky R. (1979) Establishment and characterization of Balb/c lymphoma lines with B cell properties. J. Immun. 122, 549-554.
21. Kobrin B., Milcarek C. and Morrison S. L. (1986) Sequences near the 3′ secretion-specific polyadenylation site influence levels of secretion-specific and membrane-specific IgG2b mRNA in myeloma cells. Molec. cell. Biol. 6, 1687-1697.
22. Lassman C. R., Matis S., Hall B. L., Toppmeyer D. L. and Milcarek C. (1992) Plasma cell-regulated polyadenylation at the Ig gamma 2b secretion-specific poly(A) site. J. Immun. 148, 1251-1260.
23. Law R., Kuwabard M. D., Briskin M., Fasel N., Hermanson G., Sigman D. S. and Wall R. (1987) Protein-binding site at the immunoglobulin μ-membrane polyadenylation signal: possible role in transcription termination. Proc. natn. Acad. Sci. U.S.A. 84, 9160-9164.
24. Lebman D. A., Park M. J., Fatica R. and Zhang Z. (1992) Regulation of useage of membrane and secreted 3′ termini of α mRNA differs from μ mRNA. J. Immun. 148, 3282-3289.
25. Mason J. O., Williams G. T. and Neuberger M. S. (1988) The half-life of immunoglobulin mRNA increases during B-cell differentiation: a possible role for targeting to membrane-bound polysomes. Genes Devel. 2, 1003-1011.
26. Milcarek C. and Hall B. L. (1985) Cell-specific expression of secreted versus membrane forms of immunoglobulin gamma 2b mRNA involves selective use of alternate polyadenylation sites. Molec. cell. Biol. 5, 2514-2520.
27. Morrison S. L. (1979) Sequentially derived mutants of the constant region of the heavy chain of murine immunoglobulins. J. Immun. 123, 793-800.
28. Peterson M. L. (1994) Regulated immunoglobulin (Ig) RNA processing does not require specific cis-acting sequences: non-Ig RNA can be alternatively processed in B cells and plasma cells. Molec. cell. Biol. 14, 7891-7898.
29. Peterson M. L., Gimmi E. R. and Perry R. P. (1991) The developmentally regulated shift from membrane to secreted μ mRNA production is accompanied by an increase in cleavage-polyadenylation efficiency but no measurable change in splicing efficiency. Molec. cell. Biol. 11, 2324-2327.
30. Reed D. J., Hawley J., Dang T. and Yuan D. (1994) Role of differential mRNA stability in the regulated expression of IgM and IgD. J. Immun. 152, 5330-5336.
31. Saxon A., Kurbe-Leamer M., Behle K., Max E. A. and Zhang K. (1991) Inhibition of human IgE production via FcεR-II stimulation results from a decrease in the mRNA for secreted but not membrane ε H chains. J. Immun. 147, 4000-4006.
32. Schibier U., Marcu K. B. and Perry R. P. (1978) The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells. Cell 15, 1495-1509.
33. Seipelt R. L. and Peterson M. L. (1995) Alternative processing of IgA pre-mRNA responds like IgM to alterations in the efficiency of the competing splice and cleavage-polyadenylation reactions. Molec. Immun. 32, 277-285.
34. Weiss E. A., Michael A. and Yuan D. L. (1989) Role of transcription termination in the regulation of μ mRNA expression in B lymphocytes. J. Immun. 143, 1046-1052.
35. Word C. J. and Kuehl M. (1981) Expression of surface and secreted IgG2a by a murine B-lymphoma before and after hybridization to myeloma cells. Molec. Immun. 18, 311-322.
36. Yan D.-H., Weiss E. A. and Nevins J. R. (1995) Identification of an activity in B-cell extracts that selectively impairs the formation of an immunoglobulin μs poly(A) site processing complex. Molec. cell. Biol. 15, 1901-1906.
37. Yuan D. (1984) Regulation of IgM and IgD synthesis in B lymphocytes II. Translational and post-translational events. J. Immun. 132, 1566-1570.
38. Yuan D. and Dang T. (1989) Regulation of μM and μS mRNA expression in an inducible B cell line. Molec. Immun. 26, 1059-1067.
39. Zaller D. M., Yu H. and Eckhardt L. A. (1988) Genes activated in the presence of an Ig enhancer or promoter are negatively regulated by a T lymphoma cell line. Molec. cell. Biol. 8, 1932-1939.