The C-Terminal Regulatory Domain Is the RNA 5′-Triphosphate Sensor of RIG-I

Molecular Cell

Volumn 29, Issue 2, 2008, Pages 169-179

  Cui, Sheng ^a   Eisenächer, Katharina ^b   Kirchhofer, Axel ^a   Brzózka, Krzysztof ^a   Lammens, Alfred ^a   Lammens, Katja ^a   Fujita, Takashi ^c   Conzelmann, Karl Klaus ^a   Krug, Anne ^b   Hopfner, Karl Peter ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

MOLIMMUNO; RNA

Indexed keywords

GUANINE NUCLEOTIDE EXCHANGE FACTOR; RAB PROTEIN; RETINOIC ACID INDUCIBLE PROTEIN I; VIRUS RNA; ZINC;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; EMBRYO; ENZYME ACTIVATION; HUMAN; HUMAN CELL; MUTAGENESIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ADENOSINE TRIPHOSPHATASES; ANIMALS; BINDING SITES; CELL LINE; DEAD-BOX RNA HELICASES; DIMERIZATION; HUMANS; IMMUNITY, NATURAL; INTERFERONS; POLYPHOSPHATES; PROTEIN STRUCTURE, TERTIARY; RAB GTP-BINDING PROTEINS; RNA CAPS; RNA HELICASES; RNA, VIRAL; STRUCTURAL HOMOLOGY, PROTEIN; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; ZINC;

EID: 38649089789     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcel.2007.10.032     Document Type: Article

References (31)

1. Akira S., Uematsu S., and Takeuchi O. Pathogen recognition and innate immunity. Cell 124 (2006) 783-801
2. Berger I., Fitzgerald D.J., and Richmond T.J. Baculovirus expression system for heterologous multiprotein complexes. Nat. Biotechnol. 22 (2004) 1583-1587
3. Brunger A.T., Adams P.D., Clore G.M., DeLano W.L., Gros P., Grosse-Kunstleve R.W., Jiang J.S., Kuszewski J., Nilges M., Pannu N.S., et al. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr. D Biol. Crystallogr. 54 (1998) 905-921
4. Emsley P., and Cowtan K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D Biol. Crystallogr. 60 (2004) 2126-2132
5. Gitlin L., Barchet W., Gilfillan S., Cella M., Beutler B., Flavell R.A., Diamond M.S., and Colonna M. Essential role of mda-5 in type I IFN responses to polyriboinosinic:polyribocytidylic acid and encephalomyocarditis picornavirus. Proc. Natl. Acad. Sci. USA 103 (2006) 8459-8464
6. Gorbalenya A.E., Koonin E.V., Donchenko A.P., and Blinov V.M. A novel superfamily of nucleoside triphosphate-binding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination. FEBS Lett. 235 (1988) 16-24
7. Hiscott J., Lin R., Nakhaei P., and Paz S. MasterCARD: a priceless link to innate immunity. Trends Mol. Med. 12 (2006) 53-56
8. Holm L., and Sander C. Protein structure comparison by alignment of distance matrices. J. Mol. Biol. 233 (1993) 123-138
9. Hopfner K.P., and Tainer J.A. Rad50/SMC proteins and ABC transporters: unifying concepts from high-resolution structures. Curr. Opin. Struct. Biol. 13 (2003) 249-255
10. Hopfner K.P., and Michaelis J. Mechanisms of nucleic acid translocases: lessons from structural biology and single-molecule biophysics. Curr. Opin. Struct. Biol. 17 (2007) 87-95
11. Hornung V., Ellegast J., Kim S., Brzozka K., Jung A., Kato H., Poeck H., Akira S., Conzelmann K.K., Schlee M., et al. 5′-triphosphate RNA is the ligand for RIG-I. Science 314 (2006) 994-997
12. Itzen A., Pylypenko O., Goody R.S., Alexandrov K., and Rak A. Nucleotide exchange via local protein unfolding-structure of Rab8 in complex with MSS4. EMBO J. 25 (2006) 1445-1455
13. Johnson C.L., and Gale Jr. M. CARD games between virus and host get a new player. Trends Immunol. 27 (2006) 1-4
14. Kabsch W. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants. J. Appl. Crystallogr. 21 (1993) 916-924
15. Kato H., Takeuchi O., Sato S., Yoneyama M., Yamamoto M., Matsui K., Uematsu S., Jung A., Kawai T., Ishii K.J., et al. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature 441 (2006) 101-105
16. Kawai T., and Akira S. Innate immune recognition of viral infection. Nat. Immunol. 7 (2006) 131-137
17. Kawai T., Takahashi K., Sato S., Coban C., Kumar H., Kato H., Ishii K.J., Takeuchi O., and Akira S. IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction. Nat. Immunol. 6 (2005) 981-988
18. Komuro A., and Horvath C.M. RNA- and virus-independent inhibition of antiviral signaling by RNA helicase LGP2. J. Virol. 80 (2006) 12332-12342
19. Meylan E., Curran J., Hofmann K., Moradpour D., Binder M., Bartenschlager R., and Tschopp J. Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus. Nature 437 (2005) 1167-1172
20. Meylan E., Tschopp J., and Karin M. Intracellular pattern recognition receptors in the host response. Nature 442 (2006) 39-44
21. Pichlmair A., Schulz O., Tan C.P., Naslund T.I., Liljestrom P., Weber F., and Reis e Sousa C. RIG-I-mediated antiviral responses to single-stranded RNA bearing 5′-phosphates. Science 314 (2006) 997-1001
22. Plumet S., Herschke F., Bourhis J.M., Valentin H., Longhi S., and Gerlier D. Cytosolic 5′-triphosphate ended viral leader transcript of measles virus as activator of the RIG I-mediated interferon response. PLoS ONE 2 (2007) e279 10.1371/journal.pone.0000279
23. Rothenfusser S., Goutagny N., DiPerna G., Gong M., Monks B.G., Schoenemeyer A., Yamamoto M., Akira S., and Fitzgerald K.A. The RNA helicase Lgp2 inhibits TLR-independent sensing of viral replication by retinoic acid-inducible gene-I. J. Immunol. 175 (2005) 5260-5268
24. Saito T., Hirai R., Loo Y.M., Owen D., Johnson C.L., Sinha S.C., Akira S., Fujita T., and Gale Jr. M. Regulation of innate antiviral defenses through a shared repressor domain in RIG-I and LGP2. Proc. Natl. Acad. Sci. USA 104 (2007) 582-587
25. Seth R.B., Sun L., Ea C.K., and Chen Z.J. Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3. Cell 122 (2005) 669-682
26. Seth R.B., Sun L., and Chen Z.J. Antiviral innate immunity pathways. Cell Res. 16 (2006) 141-147
27. Sumpter Jr. R., Loo Y.M., Foy E., Li K., Yoneyama M., Fujita T., Lemon S.M., and Gale Jr. M. Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I. J. Virol. 79 (2005) 2689-2699
28. Xu L.G., Wang Y.Y., Han K.J., Li L.Y., Zhai Z., and Shu H.B. VISA is an adapter protein required for virus-triggered IFN-beta signaling. Mol. Cell 19 (2005) 727-740
29. Yoneyama M., Kikuchi M., Natsukawa T., Shinobu N., Imaizumi T., Miyagishi M., Taira K., Akira S., and Fujita T. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 5 (2004) 730-737
30. Yoneyama M., Kikuchi M., Matsumoto K., Imaizumi T., Miyagishi M., Taira K., Foy E., Loo Y.M., Gale Jr. M., Akira S., et al. Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity. J. Immunol. 175 (2005) 2851-2858
31. Yu H., and Schreiber S.L. Structure of guanine-nucleotide-exchange factor human Mss4 and identification of its Rab-interacting surface. Nature 376 (1995) 788-791