Expression of two recombinant chloramphenicol acetyltransferase variants in highly reduced genome Escherichia coli strains

Biotechnology and Bioengineering

Volumn 98, Issue 5, 2007, Pages 1056-1070

  Sharma, Shamik S ^a,d   Campbell, John W ^b   Frisch, David ^b   Blattner, Frederick R ^b,c   Harcum, Sarah W ^a  

^a AMRL   (United States)

^b Scarab Genomics LLC   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

^d NEW ENGLAND BIOLABS   (United States)

Author keywords

Acetyl CoA; Metabolix flux; Minimal genome; Recombinant

Indexed keywords

BIOMASS; BIOSYNTHESIS; CELL GROWTH; ENZYME KINETICS; ESCHERICHIA COLI; PROTEINS;

ACETYL-COA; METABOLIX FLUX; MINIMAL GENOME; RECOMBINANTS;

GENE EXPRESSION;

CHLORAMPHENICOL ACETYLTRANSFERASE; ENZYME VARIANT; GLYCEROL;

ARTICLE; BACTERIAL GENOME; BACTERIAL STRAIN; BIOMASS; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE INSERTION SEQUENCE; GENETIC STABILITY; GLYCOLYSIS; GROWTH RATE; NONHUMAN; PROTEIN EXPRESSION; STRAIN DIFFERENCE; TRANSPOSON;

ACETATES; ACETYL COENZYME A; BIOMASS; BIOTECHNOLOGY; CELL COUNT; CHLORAMPHENICOL O-ACETYLTRANSFERASE; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION; GENOME, BACTERIAL; GLUCOSE; GLYCEROL; GLYCOLYSIS; ISOPROPYL THIOGALACTOSIDE; OXYGEN; PHOSPHATE ACETYLTRANSFERASE; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 36749085952     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21491     Document Type: Article

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