Effects of mutations in acetate metabolism on high-cell-density growth of Escherichia coli

Journal of Industrial Microbiology and Biotechnology

Volumn 24, Issue 6, 2000, Pages 421-430

  Contiero, J ^a   Beatty, C ^b   Kumari, S ^b   DeSanti, C L ^a   Strohl, W R ^a   Wolfe, A ^b  

^a OHIO STATE UNIVERSITY   (United States)

^b LOYOLA UNIVERSITY CHICAGO   (United States)

Author keywords

Acetate metabolism; Acetate mutants; Fed batch fermentation; Glucose controlled high cell density fermentation; RpoS

Indexed keywords

ACETIC ACID; RNA POLYMERASE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL MUTATION; BIOTECHNOLOGY; CELL DENSITY; COMPUTER PROGRAM; ENZYME SUBUNIT; ESCHERICHIA COLI; FERMENTATION; NONHUMAN; REPRODUCIBILITY; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; TRIXIS;

EID: 0033845185     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.7000014     Document Type: Article

References (34)

1. Modification of central pathway in Escherichia coli to reduce acetate accumulation by heterologous expression of the Bacillus subtilis acetolactate synthase gene
2. Improved expression of human interleukin-2 in high cell density fermentor cultures of Escherichia coli K-12 by a phosphotransacetylase mutant
3. Regulation of RssB-dependent proteolysis in Escherichia coli: A role for acetyl phosphate in a response regulator-controlled process
4. The enzymic interconversion of acetate and acetyl coenzyme A in Escherichia coli
5. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS (katF) gene
6. Effect of modified glucose uptake using genetic engineering techniques on high level recombinant protein production in Escherichia coli dense cultures
7. Regulation of acetate metabolism by protein phosphorylation in enteric bacteria
8. Effect of alteration of the acetic acid synthesis pathway on the fermentation pattern of Escherichia coli
9. Control of carbon flux to acetate excretion during growth of Escherichia coli in batch and continuous culture
10. Reduction of aerobic acetate production by Escherichia coli
11. In vivo phosphorylation of OmpR, the transcription activator of the ompF and ompC genes in Escherichia coli
12. Characterization and evaluation of a pta (phosphotransacetylase) negative mutant of Escherichia coli HB101 as production host of foreign lipase
13. Acetic acid formation in Escherichia coli fermentation
14. The central metabolic pathways of Escherichia coli: Relationship between flux and control at a branch point, efficiency of conversion to biomass and excretion of acetate
15. Production of recombinant human growth hormone in Escherichia coli. Expression of different precursors and physiological effects of glucose, acetate and salts
16. Construction of Pta-Ack pathway deletion mutants of Escherichia coli and characteristics growth profiles of the mutants in a rich medium
17. A predictive and feedback control algorithm maintains a constant glucose concentration in fed-batch fermentations
18. Glucose-stat, a glucose-controlled continuous culture
19. Acetate metabolism by Escherichia coli in high cell density fermentation
20. Over-expression of ribulose 1,5-bisphosphate carboxylase/oxygenase in glucose-controlled high cell density fermentation
21. A balanced DO-stat and its application to the control of acetic acid excretion by recombinant Escherichia coli
22. Cloning, characterization and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli
23. High cell density culture of Escherichia coli
24. Regulation in the rpoS regulon of Escherichia coli
25. Comparison of growth, acetate production and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations
26. Physiological censtraints in increasing biomass concentration of Escherichia coli B in fed-batch culture
27. Regulation of acetyl phosphale synthesis and degradation and the control of flagellar synthesis in Escherichia coli
28. High cell density cultivation of Escherichia coli at controlled specific growth rate
29. Modulation of flagellar expression in Escherichia coli by acetyl phosphate and the osmoregulator OmpR
30. Involvement of iclR and rpoS in the induction of acs, the gene for acetyl coenzyme A synthetase of Escherichia coli K- 12
31. Stoichiometric flux balance models quantitatively predict growth and metabolic by-product secretion in wild-type Escherichia coli W3110
32. Recombinant protein expression in high cell density fed-batch culture of Escherichia coli
33. Modeling of overflow metabolism in batch and fed-batch cultures of Escherichia coli