메뉴 건너뛰기




Volumn 9, Issue 1, 2005, Pages 26-34

Increasing PCR fragment stability and protein yields in a cell-free system with genetically modified Escherichia coli extracts

Author keywords

DNA instability; Escherichia coli; Polymerase chain reaction fragments

Indexed keywords

CELL EXTRACT; CHLORAMPHENICOL ACETYLTRANSFERASE; DEOXYRIBONUCLEASE I; EXODEOXYRIBONUCLEASE V;

EID: 27544487569     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000088143     Document Type: Article
Times cited : (68)

References (20)
  • 1
  • 2
    • 0035151833 scopus 로고    scopus 로고
    • Effect of sequences of the active-site dipeptides of DsbA and DsbC on in vivo folding of multidisulfide proteins in Escherichia coli
    • Bessette PH, Qiu J, Bardwell JC, Swartz JR, Georgiou G: Effect of sequences of the active-site dipeptides of DsbA and DsbC on in vivo folding of multidisulfide proteins in Escherichia coli. J Bacteriol 2001;183:980-988.
    • (2001) J Bacteriol , vol.183 , pp. 980-988
    • Bessette, P.H.1    Qiu, J.2    Bardwell, J.C.3    Swartz, J.R.4    Georgiou, G.5
  • 3
    • 0031029555 scopus 로고    scopus 로고
    • In vitro scanning saturation mutagenesis of an antibody binding pocket
    • USA
    • Burks EA, Chen G, Georgiou G, Iverson BL: In vitro scanning saturation mutagenesis of an antibody binding pocket. Proc Natl Acad Sci USA 1997;94:412-417.
    • (1997) Proc Natl Acad Sci , vol.94 , pp. 412-417
    • Burks, E.A.1    Chen, G.2    Georgiou, G.3    Iverson, B.L.4
  • 4
    • 0013892978 scopus 로고
    • Isolation and characterization of ribonuclease I mutants of Escherichia coli
    • Gesteland RF: Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol 1966;16:67-84.
    • (1966) J Mol Biol , vol.16 , pp. 67-84
    • Gesteland, R.F.1
  • 5
    • 1542720448 scopus 로고    scopus 로고
    • Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis
    • Jewett MC, Swartz J: Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis. Biotechnol Bioeng 2004;86:19-26.
    • (2004) Biotechnol Bioeng , vol.86 , pp. 19-26
    • Jewett, M.C.1    Swartz, J.2
  • 6
    • 0442289581 scopus 로고    scopus 로고
    • Prokaryotic systems for in vitro expression
    • Weiner MP, Lu Q (eds.): Westborough, Eaton
    • Jewett MC, Voloshin A, Swartz J: Prokaryotic systems for in vitro expression; in Weiner MP, Lu Q (eds.): Gene Cloning and Expression Technologies. Westborough, Eaton, 2002, pp 391-411.
    • (2002) Gene Cloning and Expression Technologies , pp. 391-411
    • Jewett, M.C.1    Voloshin, A.2    Swartz, J.3
  • 7
    • 0035921172 scopus 로고    scopus 로고
    • Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis
    • Kim DM, Swartz J: Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis. Biotechnol Bioeng 2001;74:309-316.
    • (2001) Biotechnol Bioeng , vol.74 , pp. 309-316
    • Kim, D.M.1    Swartz, J.2
  • 8
    • 0027288130 scopus 로고
    • Kinetics and processivity of ATP hydrolysis and DNA unwinding by the RecBC enzyme from Escherichia coli
    • Korangy F, Julin D: Kinetics and processivity of ATP hydrolysis and DNA unwinding by the RecBC enzyme from Escherichia coli. Biochemistry 1993;32:4873-4880.
    • (1993) Biochemistry , vol.32 , pp. 4873-4880
    • Korangy, F.1    Julin, D.2
  • 9
    • 73849161476 scopus 로고
    • The deoxyribonucleases of Escherichia coli. II: Purification and properties of a ribonucleic acid-inhibitable endonuclease
    • Lehman IR, Roussos GG, Pratt EA: The deoxyribonucleases of Escherichia coli. II: Purification and properties of a ribonucleic acid-inhibitable endonuclease. J Biol Chem 1962;237:819-828.
    • (1962) J Biol Chem , vol.237 , pp. 819-828
    • Lehman, I.R.1    Roussos, G.G.2    Pratt, E.A.3
  • 10
    • 0025737880 scopus 로고
    • Use of in vitro synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies
    • Lesley SA, Brow MAD, Burgess RR: Use of in vitro synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies. J Biol Chem 1991;266:2632-2638.
    • (1991) J Biol Chem , vol.266 , pp. 2632-2638
    • Lesley, S.A.1    Brow, M.A.D.2    Burgess, R.R.3
  • 11
    • 4644317426 scopus 로고    scopus 로고
    • Expression-PCR: From gene pools to purified proteins within one day
    • Swartz JR (ed.): New York, Springer
    • Merk H, Meschkat D, Stiege W: Expression-PCR: from gene pools to purified proteins within one day; in Swartz JR (ed.): Cell-Free Protein Expression. New York, Springer, 2003, pp 15-23.
    • (2003) Cell-Free Protein Expression , pp. 15-23
    • Merk, H.1    Meschkat, D.2    Stiege, W.3
  • 12
    • 0030043465 scopus 로고    scopus 로고
    • Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria
    • Metcalf WW, Jiang W, Daniels LL, Kim SK, Haldimann A, Wanner BL: Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 1996;35:1-13.
    • (1996) Plasmid , vol.35 , pp. 1-13
    • Metcalf, W.W.1    Jiang, W.2    Daniels, L.L.3    Kim, S.K.4    Haldimann, A.5    Wanner, B.L.6
  • 13
    • 3242686114 scopus 로고    scopus 로고
    • Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome
    • Michel-Reydellet N, Calhoun K, Swartz J: Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome. Metab Eng 2004;6:197-203.
    • (2004) Metab Eng , vol.6 , pp. 197-203
    • Michel-Reydellet, N.1    Calhoun, K.2    Swartz, J.3
  • 14
    • 0031924072 scopus 로고    scopus 로고
    • Use of bacteriophage λ recombination functions to promote gene replacement in Escherichia coli
    • Murphy KC: Use of bacteriophage λ recombination functions to promote gene replacement in Escherichia coli. J Bacteriol 1998;180:2063-2071.
    • (1998) J Bacteriol , vol.180 , pp. 2063-2071
    • Murphy, K.C.1
  • 15
    • 0034603783 scopus 로고    scopus 로고
    • PCR-mediated gene replacement in Escherichia coli
    • Murphy KC, Campellone KG, Poteete AR: PCR-mediated gene replacement in Escherichia coli. Gene 2000;246:321-330.
    • (2000) Gene , vol.246 , pp. 321-330
    • Murphy, K.C.1    Campellone, K.G.2    Poteete, A.R.3
  • 16
    • 27544511637 scopus 로고    scopus 로고
    • Generation of linear expression elements by PCR
    • Swartz JR (ed.): New York, Springer
    • Nemetz C: Generation of linear expression elements by PCR; in Swartz JR (ed.): Cell-Free Protein Expression. New York, Springer, 2003, pp 3-7.
    • (2003) Cell-Free Protein Expression , pp. 3-7
    • Nemetz, C.1
  • 17
    • 0032190229 scopus 로고    scopus 로고
    • In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation
    • Ohuchi S, Nakano H, Yamane T: In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation. Nucleic Acids Res 1998;26:4339-4346.
    • (1998) Nucleic Acids Res , vol.26 , pp. 4339-4346
    • Ohuchi, S.1    Nakano, H.2    Yamane, T.3
  • 18
    • 0015149052 scopus 로고
    • The isolation and characterization from Escherichia coli of an adenosine triphosphate-dependent deoxyribonuclease directed by rec B, C genes
    • Wright M, Buttin G, Hurwitz J: The isolation and characterization from Escherichia coli of an adenosine triphosphate-dependent deoxyribonuclease directed by rec B, C genes. J Biol Chem 1971;246:6543-6555.
    • (1971) J Biol Chem , vol.246 , pp. 6543-6555
    • Wright, M.1    Buttin, G.2    Hurwitz, J.3
  • 19
    • 0009907706 scopus 로고
    • Cell-free coupled transcription-translation system for investigation of linear DNA segments
    • USA
    • Yang HL, Ivashkiv L, Chen HZ, Zubay G, Cashel M: Cell-free coupled transcription-translation system for investigation of linear DNA segments. Proc Natl Acad Sci USA 1980;77:7029-7033.
    • (1980) Proc Natl Acad Sci , vol.77 , pp. 7029-7033
    • Yang, H.L.1    Ivashkiv, L.2    Chen, H.Z.3    Zubay, G.4    Cashel, M.5
  • 20
    • 0032477911 scopus 로고    scopus 로고
    • The 30-kDa C-terminal domain of the RecB protein is critical for the nuclease activity, but not the helicase activity, of the RecBCD enzyme from Escherichia coli
    • USA
    • Yu M, Souaya J, Julin D: The 30-kDa C-terminal domain of the RecB protein is critical for the nuclease activity, but not the helicase activity, of the RecBCD enzyme from Escherichia coli. Proc Natl Acad Sci USA 1998;95:981-986.
    • (1998) Proc Natl Acad Sci , vol.95 , pp. 981-986
    • Yu, M.1    Souaya, J.2    Julin, D.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.