On-line detection of acetate formation in Escherichia coli cultures using dissolved oxygen responses to feed transients

Biotechnology and Bioengineering

Volumn 64, Issue 5, 1999, Pages 590-598

  Åkesson, Mats ^a   Karlsson, Eva Nordberg ^b   Hagander, Per ^a   Axelsson, Jan Peter ^c   Tocaj, Anita ^b  

^a LUND INSTITUTE OF TECHNOLOGY   (Sweden)

^b LUND UNIVERSITY   (Sweden)

^c PFIZER INC   (United States)

Author keywords

Acetate; Dissolved oxygen; Escherichia coli; Mathematical model; On line detection; Transients

Indexed keywords

BATCH CELL CULTURE; DISSOLVED OXYGEN SENSORS; ESCHERICHIA COLI; GLUCOSE; MATHEMATICAL MODELS; ORGANIC COMPOUNDS; PROTEINS;

ACETATE; DISSOLVED OXYGEN; FEED TRANSIENTS; ONLINE DETECTION; RECOMBINANT PROTEIN PRODUCTION;

BIOTECHNOLOGY;

ACETIC ACID; OXYGEN;

ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; BIOREACTOR; CHEMICAL ANALYSIS; ESCHERICHIA COLI; GLUCOSE UTILIZATION; MATHEMATICAL MODEL; NONHUMAN; OXYGEN CONSUMPTION;

ESCHERICHIA COLI;

EID: 0345085733     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990905)64:5<590::AID-BIT9>3.0.CO;2-T     Document Type: Article

References (26)

1. Andersen K, Von Meyenburg K. 1980. Are growth rates of Escherichia coli in batch cultures limited by respiration? J Bacteriol 144:114-123.
2. Bauer K, Ben-Bassat A, Dawson M, De La Puente V, Neway J. 1990. Improved expression of human interleukin-2 in high-cell-density fermentor cultures of Escherichia coli K-12 by a phosphotransacetylase mutant. Appl Environ Microbiol 56:1296-1302.
3. Bech Jensen E, Carlsen S. 1990. Production of recombinant human growth hormone in Escherichia coli: Expression of different precursors and physiological effects of glucose, acetate and salts. Biotechnol Bioeng 36:1-11.
4. Benthin S, Nielsen J, Villadsen J. 1992. Anomeric specificity of glucose uptake in Lactococcus cremoris, Escherichia coli, and Saccharomyces cerevisiae: Mechanism, kinetics, and implications. Biotechnol Bioeng 40:137-146.
5. Dang NDP, Karrer DA, Dunn U. 1977. Oxygen transfer coefficients by dynamic model moment analysis. Biotechnol Bioeng 19:853-865.
6. Holme T, Arvidsson S, Lindholm B, Pavlu B. 1970. Enzymes: Laboratory-scale production. Process Biochem 5:62-66.
7. Ko Y-F, Bentley WE, Weigand WA. 1993. An integrated metabolic modeling approach to describe the energy efficiency of Escherichia coli fermentations under oxygen-limited conditions: Cellular energetics, carbon flux, and acetate production. Biotechnol Bioeng 42:843-853.
8. Ko Y-F, Bentley WE, Weigand WA. 1994. A metabolic model of cellular energetics and carbon flux during aerobic Escherichia coli fermentation. Biotechnol Bioeng 43:847-855.
9. Larsson G, Törnkvist M. 1996. Rapid sampling, cell inactivation and evaluation of low extracellular glucose concentration during fed-batch cultivation. J Biotechnol 49:69-82.
10. Lee SY. 1996. High cell-density culture of Escherichia coli. Trends Biotechnol 14:98-105.
11. Luli GW, Strohl WR. 1990. Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl Environ Microbiol 56:1004-1011.
12. Majewski RA, Domach MM. 1990. Simple constrained-optimization view of acetate overflow in E. coli. Biotechnol Bioeng 35:732-738.
13. Meyer H-P, Leist C, Fiechter A 1984. Acetate formation in continuous culture of Escherichia coli K12 D1 on defined and complex media. J Biotechnol 1:355-358.
14. Neubauer P, Häggström L, Enfors S-O. 1995. Influence of substrate oscillations on acetate formation and growth yield in Escherichia coli glucose limited fed-batch cultures. Biotechnol Bioeng 47:139-146.
15. Nordberg Karlsson E, Dahlberg L, Torto N, Gorton L, Holst O. 1998. Enzymatic specificity and hydrolysis pattern of the catalytic domain of the xylanase (xyn1) from Rhodotermus marinus. J Biotechnol 60: 23-35.
16. Paalme T, Elken R, Kahru A, Vanatalu K, Vilu R. 1997. The growth rate control in Escherichia coli at near to maximum growth rates: The A-stat approach. Antonie van Leeuwenhook 71:217-230.
17. Ramírez D, Bentley W. 1993. Enhancement of recombinant protein synthesis and stability via coordinated amino acid addition. Biotechnol Bioeng 41:557-565.
18. Reiling HE, Laurila H, Fiechter A. 1985. Mass culture of Escherichia coli: medium development for low and high density cultivation of Escherichia coli B/r in minimal and complex media. J Biotechnol 2: 191-206.
19. Schügerl K, Hitzmann B, Jurgens H, Kullick T, Ulber R, Weigal B. 1996. Challenges in integrating biosensors and FIA for on-line monitoring and control. Trends Biotechnol 14:21-31.
20. Shiloach J, Kaufman J, Guillard A, Fass R. 1996. Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21(λDE3) and Escherichia coli JM109. Biotechnol Bioeng 49:421-428.
21. Stephanopoulos G, San K-Y. 1984. Studies on on-line bioreactor identification. I. Theory. Biotechnol Bioeng 26:1176-1188.
22. Studier F, Moffatt B. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189:113-130.
23. Tocaj A, Sjöbring U, Björck L, Holst O. 1995. High level expression of Protein L, an immunoglobulin-binding protein, in Escherichia coli. J Ferment Bioeng 80(1):1-5.
24. Varma A, Boesch B, Palsson B. 1993. Stoichiometric interpretation of Escherichia coli glucose catabolism under various oxygenation rates. Appl Environ Microbiol 59:2465-2473.
25. Wang H, Cooney C, Wang D. 1977. Computer-aided baker's yeast fermentations. Biotechnol Bioeng 19:69-86.
26. Yee L, Blanch HW. 1992. Recombinant protein expression in high cell density fed-batch cultures of Escherichia coli. Bio/Technology 10: 1550-1556.