Identification of inhibitors using a cell-based assay for monitoring Golgi-resident protease activity

Analytical Biochemistry

Volumn 364, Issue 1, 2007, Pages 19-29

  Coppola, Julia M ^a   Hamilton, Christin A ^a   Bhojani, Mahaveer S ^a   Larsen, Martha J ^b   Ross, Brian D ^a   Rehemtulla, Alnawaz ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b Life Sciences Institute   (United States)

Author keywords

Alkaline phosphatase; Alzheimer's; BACE; Furin; NSAIDs; Prohormone convertase; SEAP; TGN

Indexed keywords

DIAGNOSIS; DRUG PRODUCTS; MAMMALS; MUTAGENS; NEURODEGENERATIVE DISEASES;

ALKALINE PHOSPHATASE; ALZHEIMER'S; BACE; FURIN; NSAIDS; PROHORMONE CONVERTASE; SEAP;

PHOSPHATASES;

ALKALINE PHOSPHATASE; AMYLOID BETA PROTEIN; ANTHRAX TOXIN; BETA SECRETASE; DIPHTHERIA TOXIN; FURIN; HYBRID PROTEIN; PROTEINASE; PROTEINASE INHIBITOR;

ALZHEIMER DISEASE; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; AVIAN INFLUENZA; CARCINOGENESIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; GOLGI COMPLEX; HIGH THROUGHPUT SCREENING; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; MAMMAL CELL; MONITORING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PSEUDOMONAS; QUANTITATIVE ANALYSIS; SIGNAL TRANSDUCTION; TECHNOLOGY; VIROGENESIS; VIRUS HEMORRHAGIC FEVER;

AVES; MAMMALIA; PSEUDOMONAS;

EID: 33947695615     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.01.013     Document Type: Article

References (53)

1. Patel T., Gores G.J., and Kaufmann S.H. The role of proteases during apoptosis. FASEB J. 10 (1996) 587-597
2. Nunez G., Benedict M.A., Hu Y., and Inohara N. Caspases: The proteases of the apoptotic pathway. Oncogene 17 (1998) 3237-3245
3. Gordon V.M., Klimpel K.R., Arora N., Henderson M.A., and Leppla S.H. Proteolytic activation of bacterial toxins by eukaryotic cells is performed by furin and by additional cellular proteases. Infect. Immunol. 63 (1995) 82-87
4. Taylor N.A., Van de Ven W.J., and Creemers J.W. Curbing activation: Proprotein convertases in homeostasis and pathology. FASEB J. 17 (2003) 1215-1227
5. Walsh P.N., and Ahmad S.S. Proteases in blood clotting. Essays Biochem. 38 (2002) 95-111
6. Neurath H., and Walsh K.A. Role of proteolytic enzymes in biological regulation (a review). Proc. Natl. Acad. Sci. USA 73 (1976) 3825-3832
7. Siegel R.M. Caspases at the crossroads of immune-cell life and death. Nat. Rev. Immunol. 6 (2006) 308-317
8. Steiner D.F., Docherty K., and Carroll R. Golgi/Granule processing of peptide hormone and neuropeptide precursors: A minireview. J. Cell. Biochem. 24 (1984) 121-130
9. Gluschankof P., and Cohen P. Proteolytic enzymes in the post-translational processing of polypeptide hormone precursors. Neurochem. Res. 12 (1987) 951-958
10. Molloy S.S., Anderson E.D., Jean F., and Thomas G. Bi-cycling the furin pathway: From TGN localization to pathogen activation and embryogenesis. Trends Cell. Biol. 9 (1999) 28-35
11. Halban P.A., and Irminger J.C. Sorting and processing of secretory proteins. Biochem. J. 299 (1994) 1-18
12. Reznik S.E., and Fricker L.D. Carboxypeptidases from A to Z: Implications in embryonic development and Wnt binding. Cell. Mol. Life Sci. 58 (2001) 1790-1804
13. Vassar R. β-Secretase (BACE) as a drug target for Alzheimer's disease. Adv. Drug Deliv. Rev. 54 (2002) 1589-1602
14. Nakayama K. Furin: A mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins. Biochem. J. 327 (1997) 625-635
15. Rockwell N.C., Krysan D.J., Komiyama T., and Fuller R.S. Precursor processing by kex2/furin proteases. Chem. Rev. 102 (2002) 4525-4548
16. Thomas G. Furin at the cutting edge: From protein traffic to embryogenesis and disease. Nat. Rev. Mol. Cell. Biol. 3 (2002) 753-766
17. Bassi D.E., Fu J., Lopez de Cicco R., and Klein-Szanto A.J. Proprotein convertases: "Master switches" in the regulation of tumor growth and progression. Mol. Carcinog. 44 (2005) 151-161
18. Basak A., Zhong M., Munzer J.S., Chretien M., and Seidah N.G. Implication of the proprotein convertases furin, PC5, and PC7 in the cleavage of surface glycoproteins of Hong Kong, ebola, and respiratory syncytial viruses: A comparative analysis with fluorogenic peptides. Biochem. J. 353 (2001) 537-545
19. Moulard M., and Decroly E. Maturation of HIV envelope glycoprotein precursors by cellular endoproteases. Biochim. Biophys. Acta 1469 (2000) 121-132
20. Bennett B.D., Denis P., Haniu M., Teplow D.B., Kahn S., Louis J.C., Citron M., and Vassar R. A furin-like convertase mediates propeptide cleavage of BACE, the Alzheimer's β-secretase. J. Biol. Chem. 275 (2000) 37712-37717
21. Citron M. Alzheimer's disease: Treatments in discovery and development. Nat. Neurosci. 5 Suppl. (2002) 1055-1057
22. Farzan M., Schnitzler C.E., Vasilieva N., Leung D., and Choe H. BACE2, a β-secretase homolog, cleaves at the β site and within the amyloid- β region of the amyloid-β precursor protein. Proc. Natl. Acad. Sci. USA 97 (2000) 9712-9717
23. Citron M., Oltersdorf T., Haass C., McConlogue L., Hung A.Y., Seubert P., Vigo-Pelfrey C., Lieberburg I., and Selkoe D.J. Mutation of the β-amyloid precursor protein in familial Alzheimer's disease increases β-protein production. Nature 360 (1992) 672-674
24. John V., Beck J.P., Bienkowski M.J., Sinha S., and Heinrikson R.L. Human (-secretase (BACE) and BACE inhibitors. J. Med. Chem. 46 (2003) 4625-4630
25. Chen G., Bhojani M.S., Heaford A.C., Chang D.C., Laxman B., Thomas D.G., Griffin L.B., Yu J., Coppola J.M., Giordano T.J., Lin L., Adams D., Orringer M.B., Ross B.D., Beer D.G., and Rehemtulla A. Phosphorylated FADD induces NF-κB, perturbs cell cycle, and is associated with poor outcome in lung adenocarcinomas. Proc. Natl. Acad. Sci. USA 102 (2005) 12507-12512
26. Yang T., Kondepudi A., Adams M., Kitts P., and Kain S. Quantitative detection of specific gene regulation with the Great EscAPe secreted alkaline phosphatase (SEAP) genetic reporter system. CLONTECHniques 9 (1994) 1-4
27. Zhang J.H., Chung T.D., and Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4 (1999) 67-73
28. Pei D., and Weiss S.J. Furin-dependent intracellular activation of the human stromelysin-3 zymogen. Nature 375 (1995) 244-247
29. Takahashi S., Kasai K., Hatsuzawa K., Kitamura N., Misumi Y., Ikehara Y., Murakami K., and Nakayama K. A mutation of furin causes the lack of precursor-processing activity in human colon carcinoma LoVo cells. Biochem. Biophys. Res. Commun. 195 (1993) 1019-1026
30. Hamstra D.A., and Rehemtulla A. Toward an enzyme/prodrug strategy for cancer gene therapy: Endogenous activation of carboxypeptidase A mutants by the PACE/furin family of propeptidases. Hum. Gene Ther. 10 (1999) 235-248
31. Rehemtulla A., and Kaufman R.J. Preferred sequence requirements for cleavage of pro-von Willebrand factor by propeptide-processing enzymes. Blood 79 (1992) 2349-2355
32. McGeer P.L., and McGeer E.G. Anti-inflammatory drugs in the fight against Alzheimer's disease. Ann. N.Y. Acad. Sci. 777 (1996) 213-220
33. Stewart W.F., Kawas C., Corrada M., and Metter E.J. Risk of Alzheimer's disease and duration of NSAID use. Neurology 48 (1997) 626-632
34. Seidah N.G., Chretien M., and Day R. The family of subtilisin/kexin like pro-protein and pro-hormone convertases: Divergent or shared functions. Biochimie 76 (1994) 197-209
35. Gordon V.M., Rehemtulla A., and Leppla S.H. A role for PACE4 in the proteolytic activation of anthrax toxin protective antigen. Infect. Immunol. 65 (1997) 3370-3375
36. Seidah N.G., Hamelin J., Mamarbachi M., Dong W., Tardos H., Mbikay M., Chretien M., and Day R. cDNA structure, tissue distribution, and chromosomal localization of rat PC7, a novel mammalian proprotein convertase closest to yeast kexin-like proteinases. Proc. Natl. Acad. Sci. USA 93 (1996) 3388-3393
37. 1-Antitrypsin Portland, a bioengineered serpin highly selective for furin: Application as an antipathogenic agent. Proc. Natl. Acad. Sci. USA 95 (1998) 7293-7298
38. Cameron A., Appel J., Houghten R.A., and Lindberg I. Polyarginines are potent furin inhibitors. J. Biol. Chem. 275 (2000) 36741-36749
39. Kacprzak M.M., Peinado J.R., Than M.E., Appel J., Henrich S., Lipkind G., Houghten R.A., Bode W., and Lindberg I. Inhibition of furin by polyarginine-containing peptides: Nanomolar inhibition by nona-d-arginine. J. Biol. Chem. 279 (2004) 36788-36794
40. Vey M., Schafer W., Reis B., Ohuchi R., Britt W., Garten W., Klenk H.D., and Radsak K. Proteolytic processing of human cytomegalovirus glycoprotein B (gpUL55) is mediated by the human endoprotease furin. Virology 206 (1995) 746-749
41. Podsiadlo P., Komiyama T., Fuller R.S., and Blum O. Furin inhibition by compounds of copper and zinc. J. Biol. Chem. 279 (2004) 36219-36227
42. Oh M., Kim S.Y., Oh Y.S., Choi D.Y., Sin H.J., Jung I.M., and Park W.J. Cell-based assay for β-secretase activity. Anal. Biochem. 323 (2003) 7-11
43. Citron M. Emerging Alzheimer's disease therapies: Inhibition of β-secretase. Neurobiol. Aging 23 (2002) 1017-1022
44. Citron M. β-Secretase as a target for the treatment of Alzheimer's disease. J. Neurosci. Res. 70 (2002) 373-379
45. Hardy J. The Alzheimer family of diseases: Many etiologies, one pathogenesis?. Proc. Natl. Acad. Sci. USA 94 (1997) 2095-2097
46. McGeer P.L., and McGeer E.G. NSAIDs and Alzheimer disease: Epidemiological, animal model, and clinical studies. Neurobiol. Aging (2006)
47. Akiyama H., Kondo H., Ikeda K., Kato M., and McGeer P.L. Immunohistochemical localization of neprilysin in the human cerebral cortex: Inverse association with vulnerability to amyloid-β protein (Aβ) deposition. Brain Res. 902 (2001) 277-281
48. Mackenzie I.R., and Munoz D.G. Nonsteroidal anti-inflammatory drug use and Alzheimer-type pathology in aging. Neurology 50 (1998) 986-990
49. Weggen S., Eriksen J.L., Das P., Sagi S.A., Wang R., Pietrzik C.U., Findlay K.A., Smith T.E., Murphy M.P., Bulter T., Kang D.E., Marquez-Sterling N., Golde T.E., and Koo E.H. A subset of NSAIDs lower amyloidogenic Aβ42 independently of cyclooxygenase activity. Nature 414 (2001) 212-216
50. 42 production by NSAID R-enantiomers. J. Neurochem. 83 (2002) 1009-1012
51. Zhou J.N., Liu R.Y., Kamphorst W., Hofman M.A., and Swaab D.F. Early neuropathological Alzheimer's changes in aged individuals are accompanied by decreased cerebrospinal fluid melatonin levels. J. Pineal Res. 35 (2003) 125-130
52. Heneka M.T., Klockgether T., and Feinstein D.L. Peroxisome proliferator-activated receptor-( ligands reduce neuronal inducible nitric oxide synthase expression and cell death in vivo. J. Neurosci. 20 (2000) 6862-6867
53. Takahashi Y., Hayashi I., Tominari Y., Rikimaru K., Morohashi Y., Kan T., Natsugari H., Fukuyama T., Tomita T., and Iwatsubo T. Sulindac sulfide is a noncompetitive γ-secretase inhibitor that preferentially reduces Aβ42 generation. J. Biol. Chem. 278 (2003) 18664-18670